Investigation on the effects of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa

Luber, Jaquelini; Palmieri, Marcel José; Botelho, Carolina Mastella; Rinaldo, Daniel; Andrade-Vieira, Larissa Fonseca

doi:10.1590/0001-3765201520140286

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…Leucoanthocyans were only found from guava extracts. All the components checked in this study were reported in A. hispidum (Harekrishna et al, 2010), P. guajava (Barbalho et al, 2012;Luber et al, 2015) and S. alata (Onyegeme-Okerenta et al, 2017) preparations. In our scrutiny of literature, we did not find data from phytochemical profile of V. tonoreana.…”

Section: Qualitative and Quantitative Chemical Content Of Plant Extractsmentioning

confidence: 98%

“…Therefore, P. guajava spp. stand out in a list of 71 medicinal plant of interest according to the Brazilian Unified Health System (Luber et al, 2015). A scrutiny of the literature revealed the following components from known anti-filarial plant: β-sitosterol, kaempferol, glycoside and luteolin (Nateshan and Venkateswara, 2015), consequently identified from P. guajava (Metwally et al, 2011).…”

Section: Anti-filarial Activity Against Onchocerca Ochengimentioning

confidence: 99%

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2020

Int. J. Mod. Biol. Res.

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Filariasis are parasitic diseases from which belongs onchocerciasis, a neglected tropical disease caused by Onchocerca volvulus. Owing to the lack of cure against onchocerciasis, the present study was focused on the assessment and validation of the ethnomedicinal value of some medicinal plants claimed to have anti-filarial action. First by an ethnobotanical study conducted close to Ngaoundere traditional healers (38); followed by the chemically analysis of plants quoted; and finally by their in vitro screening on bovine filaria Onchocerca ochengi at limit relevant concentrations (300 µg/mL and 4 mg/mL) to the range of herbal drug's doses locally prescribed. The mortality of worms was checked using MTT/Formazan colorimetric assay after 72 h of incubation at 37°C. It was found that, a total of 15 plant species from 10 families are reportedly being used against filariasis. Asteraceae family (50%) provided the largest species. The most frequently mentioned plants being Vernonia tonoreana (13.6) and Psidium guajava (2.63) the last one. Roots (31.58%), whole plant (28.95%) and leaves (26.32%) were reported as the dominant parts been used. The prominent methods of herbal preparation were trituration and boiling (26.32%). The most administration forms were swallowing (55.26%) and friction (23.68%). The inquired tribes mainly Podoko (52.63%) and Mboum (23.68%) used similar plants against filariasis. Phytochemical profiles of plant varied as a function of plant parts and/or extracting solvent. However, phenolic compounds, tannins, flavonoids, alkaloids, saponins and terpenoids were mostly observed. All the screened extracts exhibited 100% worms' mortality at 4 mg/mL, but only P. guajava and V. tonoreana had the same effect at 300 µg/mL. This study provides scientific evidence of traditional use of plant to treat onchocerciasis. The scored data can be useful for further research in new drug's development.

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Section: Qualitative and Quantitative Chemical Content Of Plant Extractsmentioning

confidence: 98%

Section: Anti-filarial Activity Against Onchocerca Ochengimentioning

confidence: 99%

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2020

Int. J. Mod. Biol. Res.

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“…The comet assay was performed according to Singh et al [30]. Infusions (40 g L -1 ) of each studied guava cultivar, in aliquots of 25, 50, and 300 µL, were added to 300 µL of blood diluted in phosphate-buffered saline (PBS) at 50%.…”

Section: Comet Assay: Comet Assaymentioning

confidence: 99%

Prospection of Effects of Guava Leaves Infusion: Antigenotoxic Action and Enzymatic Inhibition

César

Trento

Oliveira

et al. 2017

Natural Product Communications

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Several forms of Psidium guajava L. (guava) leaf preparations have been widely used for prevention and treatment of illnesses. However, researches about the protective action of guava's natural products on the genetic material of animal cells is scarce. Accordingly, the aim of this study was to evaluate the antigenotoxic potential of infusions of guava leaves from three cultivars (Pedro Sato, Paluma and Roxa) against DNA damage induced by Doxorubicin (DXR) in human leukocytes, and their effects on enzymatic inhibition. To assess the antigenotoxic potential of these infusions, a comet assay (single cell electrophoresis) was conducted. In addition, their inhibitory potential on phospholipase and hemolytic activities was assessed. The evaluated infusion volumes (25, 50 and 300µL) presented inhibitions around 75% of the DXR-induced damage frequencies. Their protective effect was demonstrated by arbitrary unit calculations, with values between 52.51 and 66.60 for all treatments, 250.51 for positive control (DXR), and 45.49 for phosphate buffered saline (negative control). The infusions significantly inhibited phospholipase and hemolytic activities induced by Bothrops alternatus and B. moojeni venoms, with inhibitions ≥ 50% for both activities induced by B. alternatus. The infusions of P. guajava from the cultivars analyzed present antigenotoxic potential, but also demonstrate potential of enzymatic inhibition on A 2 phospholipases and proteases.

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Phytotoxicity and cytogenotoxicity of hydroalcoholic extracts from Solanum muricatum Ait. and Solanum betaceum Cav. (Solanaceae) in the plant model Lactuca sativa

Santos

Carvalho

Silveira

et al. 2018

Environ Sci Pollut Res

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Plants are rich in biologically active compounds. They can be explored for the production of bioherbicides. In this context, the present work aimed to evaluate the allelopathic effect of hydroalcoholic extracts from two Solanaceae species: Solanum muricatum Ait. and Solanum betaceum Cav. For this end, we conducted phytochemical screening and biological assays, determining the effects of the extracts on germination, early development, cell cycle, and DNA fragmentation in plantlets and meristematic cells of the plant model Lactuca sativa L. (lettuce). The percentage of seeds germinated under effect of S. muricatum extract did not differ from the control, but plantlet growth was reduced at the highest concentrations. For S. betaceum extract, dose dependence was observed for both germination and plantlet development, with the highest concentrations inhibiting germination. The growth curves revealed the concentrations of 2.06 and 1.93 g/L for S. muricatum and S. betaceum extracts, respectively, as those reducing 50% of root growth (RG). At these concentrations, both extracts presented mitodepressive effect, besides inducing significant increase in the frequency of condensed nuclei, associated to DNA fragmentation and cytoplasmic shrinkage. The frequency of chromosome alterations was not significant. We further discuss the mechanisms of action related to the chemical composition of the extracts, which presented organic acids, reducing sugars, proteins, amino acids, and tannins, besides catechins and flavonoids, only found in the extract of S. betaceum.

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Investigation on the effects of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa

Cited by 8 publications

References 34 publications

Untitled

Untitled

Prospection of Effects of Guava Leaves Infusion: Antigenotoxic Action and Enzymatic Inhibition

Phytotoxicity and cytogenotoxicity of hydroalcoholic extracts from Solanum muricatum Ait. and Solanum betaceum Cav. (Solanaceae) in the plant model Lactuca sativa

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