Investigation on the Effects of UV Radiation on Physiological Characteristics of Moringa oleifera Lam. in vitro and in situ

Oraibi, Asma G.

doi:10.9734/bji/2017/35996

Cited by 2 publications

(1 citation statement)

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“…Carbohydrate concentrations were determined based on the phenol sulfuric acid method.25 mg of the dried tissues were homogenized with sterilized distilled water (Ajax, Australia) The filtrate was treated with 3 ml of 5% phenol and 3 ml of 98%sulfuric acid (BDH, England); then, the mixture was incubated for 20 min at 30°C, and absorbance at 485 nm was determined by a spectrophotometer [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System

2022

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There are increasing needs for developing nontoxic, low-cost, high-yield, and eco-friendly procedures for manufacturing nanoparticles. Nanobiotechnology can be used in food security for improving crop production; nanoparticles could enhance the growth and yield of different crop plants; therefore, this work aimed to improve a new nutrition formula of a hydroponic system using green biosynthesis of silver nanoparticles and Malva parviflora aqueous extract. Results shown that AFM image of AgNP surface morphology provides good indicator for biosynthesizing AgNPs. UV-vis spectroscopy showed the presence of silver elements that proved the reduction of silver ion to an element in the presence of plant extract functional groups which act as a reduction reaction capping agent. AgNPs formation from 1 mM of AgNo3 and Malva parviflora filtrate can easily be characterized through visual observations by the change in the color of the reaction mixture from green to yellowish-brown. SEM showed that most of the Ag nanoparticles were spherical in shape, well dispersed, and were either arranged in clusters of particles with each other, or as small particles, and have been identified in a size range of 12–63 nm. The EDX characterization exhibited that the highest proportion of the element composition was for silver weighting (34.11%) in nanoparticle. Other elements such as aluminum (12.28%), carbon (8.62%), hafnium (18.12%), nitrogen (9.34%), sodium (10.01%), and oxygen (7.52%) may arise from Malva parviflora extract. Also, peroxidase and catalase enzyme activity, cabbage crop seedlings, fresh and dry weights, and proline and carbohydrate concentrations were significantly increased with the increase of biosynthesized AgNP concentrations but up to limit.

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Section: Methodsmentioning

confidence: 99%

Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System

2022

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Effect of UV-C Radiation on Egyptian Henbane (Hyoscyamus muticus L.) Callus Growth and Biochemical Components

Aboseidah¹

2019

HJSC

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Physiological impact of different exposure periods (1, 2 and 3 h) of UV-C (253.7 nm) on alkaloids and hyoscyamine production in 21-days aged callus of Egyptian henbane was investigated. Results showed that the concentration of total alkaloids was increased by 2.7 and 2.3 times after exposure to UV-C for 2 or 3 h, respectively more than unstressed callus. Also, data of HPTLC revealed that 2 h exposed-callus accumulated the highest amount of hyoscyamine which similar to the amount in wild leaves but it was more 3.1 times than control. Increment of alkaloids concentration was attributed with high accumulation of non-enzymatic antioxidants as free amino acids, free phenolics and glutathione and increased of peroxidase and superoxide dismutase activity as well as reduction of H 2 O 2 concentration. Also, 2 h exposed-callus to UV-C showed high expression of electrophoretic protein bands with molecular weights 109, 42 and 29 KDa. All exposure times to UV-C increased both fresh and dry weight of callus compared to unstressed one. Short exposure time of UV-C enhanced the amount of Chl. a and b in callus than other treatments. All exposure periods of UV-C decreased the concentration of carotenoids, anthocyanine, proline and total carbohydrates than control. Long exposure period of UV-C modified the callus cells shape by induction large spherical cells and lysigenous intercellular spaces. It can be concluded that 2 h exposure time of UV-C is effective treatment for enhancement both alkaloids and hyoscyamine content in callus of Egyptian henbane.

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Investigation on the Effects of UV Radiation on Physiological Characteristics of Moringa oleifera Lam. in vitro and in situ

Cited by 2 publications

References 0 publications

Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System

Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System

Effect of UV-C Radiation on Egyptian Henbane (Hyoscyamus muticus L.) Callus Growth and Biochemical Components

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