Investigation on the effect of static magnetic field up to 30 mT on viability percent, proliferation rate and IC₅₀of HeLa and fibroblast cells

Abstract

We have investigated the effects of static magnetic field (SMF) on the viability of the human cervical cancer (HeLa) cell line and fibroblast cells. The cells were cultured in DMEM medium and treated several times (24, 48,72 and 96 h) and at several intensities (5, 10, 20 and 30 mT) of magnetic field (MF). The cytotoxicity and cell viability percent in treated cells were performed using MTT assay by evaluating mitochondrial dehydrogenase activity. The MF ability on inducing cell death or inhibiting biochemical function was reported as cell death percent. The results showed that the increase of MF intensity and the time that cells were exposed to this treatment increased sharply cell death percent and proliferation rate in HeLa cell compare to fibroblast cells. Our data suggest that SMF biological effects on cell death were different in our selected targets. Cell type and time of exposure have been therefore found to be significant factors. These findings could be used to improve new effective method using SMF in conjunction with the common therapeutic approaches.

Effects of Constant Magnetic Field to the Proliferation Rate of Human Fibroblasts Grown onto Different Substrates: Tissue Culture Polystyrene, Polyacrylamide Hydrogel and Ferrogels γ-Fe2O3 Magnetic Nanoparticles

“…Some evidence showed the inhibitory effect of magnetic field on cell proliferation in vitro. The growth of human skin fibroblasts [ 16 , 20 ], of adipose derived stem cells (ACSs) [ 21 ] and of malignant melanoma cells [ 20 ] and cervix carcinoma lines [ 22 ] was suppressed at relatively weak magnetic field 1–5 kOe. A strong magnetic field of 70 kOe showed the same impact on melanoma, ovarian carcinoma and lymphoma lines [ 23 ].…”

Effect of static magnetic field with quercetin and hesperetin on MCF-7 and MDA MB-231 breast cancer cells

“…Nevertheless, magnetotherapy have been concluded to alter cancer cell biology both in vitro and in vivo [13,15,16,17]. Although, some molecular pathways are suggested for the effect of SMF on various cellular functions; exact mechanisms are unknown yet [18].…”

Enhanced osteogenic differentiation of human bone–derived mesenchymal stem cells in 3‐dimensional printed porous titanium scaffolds by static magnetic field through up‐regulating Smad4

“…Conversely, the positive effect of SMF (3-50 mT) on BMSCs has also been reported by Kim et al (21). Until now, the resulting effect was considered to be associated with the cell type, time of exposure, and initial cell densities (22,23). The proliferation of MG63 cells showed negative and positive results when they were seeded at different initial densities.…”