Effects of Constant Magnetic Field to the Proliferation Rate of Human Fibroblasts Grown onto Different Substrates: Tissue Culture Polystyrene, Polyacrylamide Hydrogel and Ferrogels γ-Fe2O3 Magnetic Nanoparticles

Abstract

The static magnetic field was shown to affect the proliferation, adhesion and differentiation of various types of cells, making it a helpful tool for regenerative medicine, though the mechanism of its impact on cells is not completely understood. In this work, we have designed and tested a magnetic system consisting of an equidistant set of the similar commercial permanent magnets (6 × 4 assay) in order to get insight on the potential of its experimental usage in the biological studies with cells culturing in a magnetic field. Human dermal fibroblasts, which are widely applied in regenerative medicine, were used for the comparative study of their proliferation rate on tissue culture polystyrene (TCPS) and on the polyacrylamide ferrogels with 0.00, 0.63 and 1.19 wt % concentrations of γ-Fe2O3 magnetic nanoparticles obtained by the well-established technique of laser target evaporation. We used either the same batch as in previously performed but different biological experiments or the same fabrication conditions for fabrication of the nanoparticles. This adds special value to the understanding of the mechanisms of nanoparticles contributions to the processes occurring in the living systems in their presence. The magnetic field increased human dermal fibroblast cell proliferation rate on TCPS, but, at the same time, it suppressed the growth of fibroblasts on blank gel and on polyacrylamide ferrogels. However, the proliferation rate of cells on ferrogels positively correlated with the concentration of nanoparticles. Such a dependence was observed both for cell proliferation without the application of the magnetic field and under the exposure to the constant magnetic field.

Magnetically stimulated cryogels to enhance osteogenic and chondrogenic differentiation of stem cells

“…There is a rapid increase in M-Gel and batch magnet applied groups, especially for comparably low magnetic strength. In agreement with the previous results, the higher cell densities in batch magnet applied groups may due to the stimulation of the cell division by magneto-mechanical stress generated by vibrated magnetic particles (Zablotskii et al, 2016; Blyakhman et al, 2020).…”

Magnetic Properties of Iron Oxide Nanoparticles Do Not Essentially Contribute to Ferrogel Biocompatibility

“…Existing techniques are not adequate for quantitative evaluation of the structural arrangement of the MNPs or ANPs inside the gel. However, understanding the internal structure of filed gels is important, and we used earlier developed techniques for the evaluation of the structure of dried composites with MNPs and AMPs [ 10 , 13 ].…”

“…The present study is a special step allowing a comparative evaluation of the contributions of MNPs and ANPs. This comparison finally became possible because of our research works related to the biological activity of cells at hydrogels and ferrogels in similar experimental conditions [ 8 , 10 , 11 , 12 , 13 ]. In this series of experimental studies, we used human fibroblasts taken from one patient, magnetic nanoparticles of iron oxide Fe 2 O 3 from the same batch, PAAm gel with the same networking, the same procedures, and experimental techniques.…”

“…The lines of human dermal fibroblasts were obtained from donor skin, as described previously [ 10 , 13 , 14 ]. Briefly, tissue biopsies were collected from patients (who have given the informed contest) during surgery.…”

“…Among others, magnetic soft materials such as ferrogels (FG) have demonstrated promising applications in biomedicine due to the ability to change their physical properties in response to an external magnetic field [ 3 , 4 ] or to be used as the components of the addressed delivery by the gradient external magnetic field both the encapsulated drugs and soft implants [ 5 ]. Furthermore, a magnetic field per se may stimulate the biological activity of certain types of cells [ 6 , 7 ] by the enhancement of cell adhesion, proliferation, differentiation as far as modifying properties of the fluids used for cell cultivation [ 8 , 9 , 10 ].…”