Investigation on lignocellulosic saccharification and characterization of haloalkaline solvent tolerant endo-1,4 β-d-xylanase from Halomonas meridiana APCMST-KS4

Palavesam, A.

doi:10.1016/j.bcab.2015.09.007

Cited by 10 publications

(3 citation statements)

References 43 publications

(51 reference statements)

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“…Tween 20, dithiothreitol (DTT), and Triton X-100 increased the activity of RuXyn by 36.0, 12.0, and 10.5%, respectively. Similarly, Tween 20 has enhanced the activities of some xylanases in the previous studies. , As a surfactant, Tween 20 had previously been recognized to be a good and nontoxic enhancer for enzymatic cellulose hydrolysis in which the xylanase from Thermotoga naphthophila was significantly inhibited by the two inhibitors.…”

Section: Results and Discussionmentioning

confidence: 82%

“…Similarly, Tween 20 has enhanced the activities of some xylanases in the previous studies. 45,46 As a surfactant, Tween 20 had previously been recognized to be a good and nontoxic enhancer for enzymatic cellulose hydrolysis. 47 RuXyn activity was inhibited by SDS and EDTA by 87.9 and 74.7%, respectively, which was consistent with the study by Hamid and Aftab (2019), 37 in which the xylanase from Thermotoga naphthophila was significantly inhibited by the two inhibitors.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of a New Xylanase Found in the Rumen Metagenome and Its Effects on the Hydrolysis of Wheat

Zhao

2022

J. Agric. Food Chem.

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Wheat is the main ingredient of poultry diet, but its xylan has an adverse impact on poultry production. A novel xylanase from beef cattle rumen metagenome (RuXyn) and its effect on the wheat hydrolysis were investigated in the present study. The RuXyn coded for 377 amino acids and exhibited low identity (<40%) to previously reported proteins. The RuXyn was heterologously expressed in Escherichia coli and showed maximum activity at pH 6.0 and 40 °C. The activity of RuXyn could be increased by 79.8 and 36.0% in the presence of Ca 2+ and Tween 20, respectively. The soluble xylan and insoluble xylan in wheat could be effectively degraded by RuXyn and xylooligosaccharides produced accounting for more than 80% of the products. This study demonstrates that RuXyn has substantial potential to improve the application of wheat in poultry production by degrading wheat xylan and the accompanying xylooligosaccharides produced.

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Section: Results and Discussionmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Characterization of a New Xylanase Found in the Rumen Metagenome and Its Effects on the Hydrolysis of Wheat

Zhao

2022

J. Agric. Food Chem.

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“…Generally, it can be said that the xylanase was not affected much by the metal ions, as it retained >70% of its enzymatic activity in the presence of most of the ions investigated, at both 1 mM and 10 mM concentrations. The positive effects of Co 2+ and Mg 2+ on the activity of xylanase from Aspergillus ochraceus [ 42 ] and Halomonas meridiana [ 43 ], respectively, have been reported. Similarly, previous research has also highlighted the inhibitory effects of Ag 2+ and Na 2+ on xylanases from Paenibacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen

Amobonye

Bhagwat

Singh

2021

JoF

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Beauveria bassiana is an entomopathogenic fungus widely used as a biopesticide for insect control; it has also been shown to exist as an endophyte, promoting plant growth in many instances. This study highlights an alternative potential of the fungus; in the production of an industrially important biocatalyst, xylanase. In this regard, Beauveria bassiana SAN01 xylanase was purified to homogeneity and subsequently characterized. The purified xylanase was found to have a specific activity of 324.2 Umg−1 and an estimated molecular mass of ~37 kDa. In addition, it demonstrated optimal activity at pH 6.0 and 45 °C while obeying Michaelis–Menton kinetics towards beechwood xylan with apparent Km, Vmax and kcat of 1.98 mgmL−1, 6.65 μM min−1 and 0.62 s−1 respectively. The enzyme activity was strongly inhibited by Ag2+ and Fe3+ while it was significantly enhanced by Co2+ and Mg2+. Furthermore, the xylanase was shown to effectively deink wastepaper at an optimal rate of 106.72% through its enzymatic disassociation of the fiber-ink bonds as demonstrated by scanning electron microscopy and infrared spectroscopy. This is the first study to demonstrate the biotechnological application of a homogeneously purified glycosyl hydrolase from B. bassiana.

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Extremozyme‐Based Technology for Biofuel Generation

Souii

Ghorrab

Hammami

et al. 2022

Biomolecules From Natural Sources

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Investigation on lignocellulosic saccharification and characterization of haloalkaline solvent tolerant endo-1,4 β-d-xylanase from Halomonas meridiana APCMST-KS4

Cited by 10 publications

References 43 publications

Characterization of a New Xylanase Found in the Rumen Metagenome and Its Effects on the Hydrolysis of Wheat

Characterization of a New Xylanase Found in the Rumen Metagenome and Its Effects on the Hydrolysis of Wheat

Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen

Extremozyme‐Based Technology for Biofuel Generation

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