Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system

Mobika, J.; Rajkumar, M.; Sibi, Srinath; Priya, V. Nithya

doi:10.1016/j.saa.2020.118836

Cited by 27 publications

(8 citation statements)

References 56 publications

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“…This change demonstrated that during the microwave heating, more tyrosine phenolic hydroxyl groups were exposed to a polar environment, enhancing moderate or weak hydrogen bonding. The α‐helix of MPs is stabilised by intra‐chain hydrogen bonds, while the β‐sheet is stabilised by interchain hydrogen bonds (Mobika et al ., 2021). In the microwave heating process, the protein molecules gradually unfolded, and the spatial conformation of the MPs changed, causing the destruction of part of the hydrogen bonds.…”

Section: Resultsmentioning

confidence: 99%

Effect of microwave heating time on the gel properties of chicken myofibrillar proteins and their formation mechanism

Wei

Kong

et al. 2022

Int J of Food Sci Tech

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Summary The present work was conducted to illustrate the mechanism of gel formation of myofibrillar proteins (MPs) under different microwave heating times. The results showed that the denaturation enthalpy (ΔH) of the MPs significantly decreased when the heating time increased from 3 to 9 s and then completely disappeared as the heating time progressed, indicating that the MPs gradually denatured and subsequently aggregated with increasing heating time, which was further verified by the changes in the secondary structure, electrophoretic bands, and gel properties (e.g., water holding capacity and textural profiles) of the MPs. Microstructural images indicated that the MP gel formed under 12 s had the most compact network, indicating that extended microwave heating time could induce quality deterioration of MP gels. Moreover, the hydrophobic forces, electrostatic forces, and disulphide bonds of the MPs gradually intensified with increasing microwave heating time, suggesting that both non‐covalent and covalent bonds could promote molecular denaturation and subsequent aggregation of MPs. In addition, correlation analysis revealed that the changes in the molecular conformation of MPs induced by different microwave heating times could effectively regulate the formation of MP gels and their related properties.

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Section: Resultsmentioning

confidence: 99%

Effect of microwave heating time on the gel properties of chicken myofibrillar proteins and their formation mechanism

Wei

Kong

et al. 2022

Int J of Food Sci Tech

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“…According to our molecular docking results, hydrogen bonds represented the main forces between SHP and BAs. Hydrogen bond forms are characterised by hydroxyl associations which may be rapidly, non‐invasively and quantitatively examined using FT‐IR (Takahashi & Noguchi, 2007; Mobika et al ., 2021), the absorption peak areas of hydrogen bonds (–OH stretching region) in the 3100–3600 cm −1 range were quantitatively assessed (Fig. 5a).…”

Section: Resultsmentioning

confidence: 99%

Molecular docking and dynamic insights on the adsorption effects of soy hull polysaccharides on bile acids

Wang

Yang

Xue

et al. 2022

Int J of Food Sci Tech

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Summary Molecular docking is a powerful tool that visualises interactions between receptors and ligands. This technique and experimental studies were used to explore the adsorption effects of soy hull polysaccharides (SHP) on bile acids (BAs). Docking sites and interactions between SHP and six BAs (deoxycholic acid, lycholic acid, taurine cholic acid, chenodeoxycholic acid, glycocholic acid and glycodesoxycholic acid) were identified by molecular docking. The docking sites were mainly O and H atoms, and interaction forces were mainly facilitated by hydrogen bonds and hydrophobic interactions, with hydrogen bonds being the main bond type. BAs‐binding studies, Fourier‐transform infrared spectroscopy and zeta potential results showed that hydrogen bonding was the main force mediating SHP and BAs adsorption. The hydrogen bonding was positively proportional to the adsorption capacity of BAs and negatively proportional to the absolute value of zeta potential. The molecular dynamic simulation of glycocholic acid and SHP was also performed. Hydrogen bond spacing and root mean square deviation analyses indicated that the glycocholic acid and SHP combination was stable. This study has the significance of preventing cardiovascular diseases by revealing the adsorption effects of SHP on bile acids BAs.

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“…A few previous studies have proven that the negatively charged SA could interact electrostatically with the positively charged microdomains of BSA 40,42 . In the present work, real-time zeta potential analysis showed that the net charge of the PTB and PTB@SA(0.10) colloids gradually tended to zero as the reaction proceeded (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Rectifying disorder of extracellular matrix to suppress urethral stricture by protein nanofilm-controlled drug delivery from urinary catheter

Tian

Delai

Guan

et al. 2022

Preprint

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Urethral stricture, which afflicts both patients and urologists, involves excessive deposition of extracellular matrix in the submucosal and periurethral tissues. Although various anti-fibrotic drugs have been applied to urethral stricture by irrigation or submucosal injection, their clinical feasibility and effectiveness are limited. To prevent stricture after endoscopic therapy, urethroplasty, and traumatic catheterization, we designed a protein-based nanofilm-controlled drug delivery system and assembled it on the catheter to target inordinate extracellular matrix deposition. This approach is simple to manufacture and simultaneously integrates excellent anti-biofilm property into a urinary catheter in one step. Stable and controlled drug delivery over tens of days ensures optimal efficacy and negligible side effects. Anti-fibrotic catheters maintain extracellular matrix homeostasis by reducing fibroblast-derived collagen production and enhancing metalloproteinase 1-induced collagen degradation in a rabbit model of urethral injury, resulting in the greatest improvement in lumen stenosis than other topical therapies for urethral stricture prevention. Such biocompatible coating with antibacterial contamination and sustained-drug-release functionality could also be an advanced paradigm for a range of biomedical applications.

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Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system

Cited by 27 publications

References 56 publications

Effect of microwave heating time on the gel properties of chicken myofibrillar proteins and their formation mechanism

Effect of microwave heating time on the gel properties of chicken myofibrillar proteins and their formation mechanism

Molecular docking and dynamic insights on the adsorption effects of soy hull polysaccharides on bile acids

Rectifying disorder of extracellular matrix to suppress urethral stricture by protein nanofilm-controlled drug delivery from urinary catheter

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