Investigation of topography effect on antibacterial properties and biocompatibility of nanohydroxyapatites activated with zinc and copper ions: In vitro study of colloids, hydrogel scaffolds and pellets

Sobierajska, Paulina; Nowak, Nicole; Rewak‐Soroczyńska, Justyna; Targońska, Sara; Lewińska, Agnieszka; Grosman, Lukasz; Wiglusz, Rafał J.

doi:10.1016/j.msec.2021.112547

Cited by 16 publications

(15 citation statements)

References 90 publications

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“…Furthermore, the Na signal has also been detected at locations 498 and 1072 eV, which could be presented as a trace element during the washing process. The carbon signal at 282 eV might be due to carbon compounds adsorbed from the air, characteristic of apatite matrices [14]. The results further demonstrated that SO 4 -HA contained the presence of sulphur, ensuring the successful doping of sulphate ions in the HA lattice.…”

Section: Resultsmentioning

confidence: 72%

Mesoporous Sulphate Doped Hydroxyapatite Nanoparticles for Controlled Release of Proteins

Alshemary¹

2022

Ceramics - Silikaty

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The purpose of this study is to synthesize and characterize the mesoporous nanostructure of Hydroxyapatite (HA), evaluate the impact of the addition of sulphate ions (SO 4 2-) on the microstructure properties of HA, and assess the ability of the prepared materials to be used as a carrier for Bovine serum albumin (BSA) protein. This study successfully synthesized 0.07 mole of SO 4 2substituted HA using the reflux microwave-assisted wet precipitation method and fully characterized it using modern techniques. In particular, XRD, XPS, Raman, FESEM, and BET techniques were used to explore the microstructure properties of materials. The results shows that the addition of SO 4 2ions amplified the lattice parameters and surface area of HA, and the particles shape was changed from semi-spherical to rod like shape. Adsorption and desorption of protein molecules were evaluated using BSA protein for 24 h at 37 °C. BSA loading capacity and release behaviours were significantly improved with the incorporation of SO 4 2ions. The SO 4 -HA materials hold a promising future for being used as a carrier for protein molecules.

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Section: Resultsmentioning

confidence: 72%

Mesoporous Sulphate Doped Hydroxyapatite Nanoparticles for Controlled Release of Proteins

Alshemary¹

2022

Ceramics - Silikaty

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“…Additionally, Chai et al [ 46 ] observed no difference in the number of MG63 osteoblast-like cells with stainless steel 317L-Cu alloy when compared to Ti-6Al-4V and stainless steel 317L. Also, in Sobierajska et al's work [ 47 ], nanohydroxyapatites (nHAp) doped with copper and/or zinc ions were investigated for the assessment of its biocompatibility. Three forms of material with diverse surfaces were tested: nanopowder in colloidal suspension, galactose hydrogel (3,6-anhydro- α -l-galacto- β -d-galactan) scaffold, and pellet.…”

Section: Discussionmentioning

confidence: 99%

“…In Sobierajska et al's work [ 47 ], nanohydroxyapatites (nHAp) doped with copper and/or zinc ions were investigated for the assessment of its biocompatibility. Three forms of material with diverse surfaces were tested: nanopowder in colloidal suspension, galactose hydrogel (3,6-anhydro- α -l-galacto- β -d-galactan) scaffold, and pellet.…”

Section: Discussionmentioning

confidence: 99%

Osteoblastic Cell Responses of Copper Nanoparticle Coatings on Ti-6Al-7Nb Alloy Using Electrophoretic Deposition Method

Hameed

Hasan

Luddin

et al. 2022

BioMed Research International

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Aim. To investigate and compare the cell cytotoxicity, proliferation, cell attachment, and morphology of human fetal osteoblasts (hFOB) cells of coated samples (titanium nanocopper (Ti Cu), titanium nanohydroxyapatite (Ti HA) and titanium nanocopper ion doped hydroxyapatite (Ti Cu/HA) and uncoated samples (Ti) in order to assess the suitability of these surface modifications on Ti-6Al-7Nb for dental implant application. Materials and Methods. The cytotoxicity was studied by examining the hFOB cell response by MTT assessment. The cell morphology was evaluated by inverted microscopy and observed under scanning electronic microscopy (SEM). Results. MTT assay results displayed that the Cu content on the surface of Ti-6Al-7Nb alloys did not produce any cytotoxic effect on cell viability. The cell viability rate in all samples ranges from 97% to 126%, indicating that hFOB cells grew at a high proliferation rate. However, no significant differences in cell viability were observed between Ti and Ti Cu and between Ti HA and Ti Cu/HA groups. Microscopic examination demonstrated no difference in the cell morphology of hFOB among all samples. In addition, SEM observation indicated favorable adhesion and spreading of the cells on the coated and uncoated samples. Conclusions. The surface modification of Ti-6Al-7Nb alloy with Cu, HA, and Cu/HA exhibits good cell biocompatibility, and the Cu has no influence on the cell proliferation and differentiation of hFOB.

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“…Although some changes in the biofilm architecture could be seen after co-doping with Zn 2+ ions, it should be noted that the most probable reason for such slight alterations is the difference between particular pellets’ surfaces. In our previous research, it was proved that generally the surface of hydroxyapatite pellet is smooth, which prevents bacteria adherence but when the surface is “rough” (more porous), the bacterial (and cellular) attachment is greater [ 19 ]. Thus, it can be assumed that the differences observed between particular materials in the present research may be the result of differences in the pellets’ surfaces which cannot be avoided using the hand press in the preparation process.…”

Section: Discussionmentioning

confidence: 99%

“…Combining apatites with antimicrobial ions results in obtaining a non-toxic, biocompatible porous material with the ability to prevent microbial growth. Apart from silver, which is often described as genotoxic and cytotoxic, such metals as gold, zinc, magnesium, iron or copper are reported to possess antimicrobial activity, especially against Gram-negative species, generally considered as more resistant to common antimicrobials [ 5 , 17 , 18 , 19 ]. On the other hand, it has been proved that nanoparticles are less active against Gram-positive bacteria.…”

Section: Introductionmentioning

confidence: 99%

The Study of Nanosized Silicate-Substituted Hydroxyapatites Co-Doped with Sr2+ and Zn2+ Ions Related to Their Influence on Biological Activities

Rewak‐Soroczyńska

Nowak

Targońska

et al. 2022

CIMB

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Nanosized silicate-substituted hydroxyapatites, characterized by the general formula Ca9.8−x−nSrnZnx(PO4)6−y(SiO4)y(OH)2 (where: n = 0.2 [mol%]; x = 0.5–3.5 [mol%]; y = 4–5 [mol%]), co-doped with Zn2+ and Sr2+ ions, were synthesized with the help of a microwave-assisted hydrothermal technique. The structural properties were determined using XRD (X-ray powder diffraction) and Fourier-transformed infrared spectroscopy (FT-IR). The morphology, size and shape of biomaterials were detected using scanning electron microscopy techniques (SEM). The reference strains of Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa were used to assess bacterial survivability and the impact on biofilm formation in the presence of nanosilicate-substituted strontium-hydroxyapatites. Safety evaluation was also performed using the standard cytotoxicity test (MTT) and hemolysis assay. Moreover, the mutagenic potential of the materials was assessed (Ames test). The obtained results suggest the dose-dependent antibacterial activity of nanomaterials, especially observed for samples doped with 3.5 mol% Zn2+ ions. Moreover, the modification with five SiO4 groups enhanced the antibacterial effect; however, a rise in the toxicity was observed as well. No harmful activity was detected in the hemolysis assay as well as in the mutagenic assay (Ames test).

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Investigation of topography effect on antibacterial properties and biocompatibility of nanohydroxyapatites activated with zinc and copper ions: In vitro study of colloids, hydrogel scaffolds and pellets

Cited by 16 publications

References 90 publications

Mesoporous Sulphate Doped Hydroxyapatite Nanoparticles for Controlled Release of Proteins

Mesoporous Sulphate Doped Hydroxyapatite Nanoparticles for Controlled Release of Proteins

Osteoblastic Cell Responses of Copper Nanoparticle Coatings on Ti-6Al-7Nb Alloy Using Electrophoretic Deposition Method

The Study of Nanosized Silicate-Substituted Hydroxyapatites Co-Doped with Sr2+ and Zn2+ Ions Related to Their Influence on Biological Activities

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