Investigation of the relationship between packing methods and efficiency of preparative columns

Klawiter, Jan; Kamiński, Marian; Kowalczyk, J.

doi:10.1016/s0021-9673(00)82413-9

Cited by 22 publications

(6 citation statements)

References 23 publications

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“…Dry packing or slurry packing of large diameter columns is more difficult than conventional diameter columns due to the scale of the packing process and the high volume flow rates and pressures required to consolidate the column bed [573,592,593,[598][599][600][601]. In fact very little information has been published on the techniques employed but it is an established fact that columns of large internal diameter are less stable than analytical-sized columns.…”

Section: High Pressure Preparative Liquid Chromatographymentioning

confidence: 99%

The Column in Liquid Chromatography

Poole

1991

Chromatography Today

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Section: High Pressure Preparative Liquid Chromatographymentioning

confidence: 99%

The Column in Liquid Chromatography

Poole

1991

Chromatography Today

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“…7 Different packing methods and conditions can result in beds that have different structural characteristics. [8][9][10] Best practices of different packing methods can be found in the respective vendor application notes and industry guidelines. 4 The packing technique applied depends on several critical factors such as the mechanical and physical properties of the resin (including rigidity, particle shape, shear resistance, density, and size) as well as hardware capabilities.…”

Section: Introductionmentioning

confidence: 99%

“…Different packing methods and conditions can result in beds that have different structural characteristics . Best practices of different packing methods can be found in the respective vendor application notes and industry guidelines .…”

Section: Introductionmentioning

confidence: 99%

Packing of large‐scale chromatography columns with irregularly shaped glass based resins using a stop‐flow method

Siu¹,

Chia²,

Mok

et al. 2014

Biotechnology Progress

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Rigid chromatography resins, such as controlled pore glass based adsorbents, offer the advantage of high permeability and a linear pressure-flow relationship irrespective of column diameter which improves process time and maximizes productivity. However, the rigidity and irregularly shaped nature of these resins often present challenges in achieving consistent and uniform packed beds as formation of bridges between resin particles can hinder bed consolidation. The standard flow-pack method when applied to irregularly shaped particles does not yield well-consolidated packed beds, resulting in formation of a head space and increased band broadening during operation. Vibration packing methods requiring the use of pneumatically driven vibrators are recommended to achieve full packed bed consolidation but limitations in manufacturing facilities and equipment may prevent the implementation of such devices. The stop-flow packing method was developed as an improvement over the flow-pack method to overcome these limitations and to improve bed consolidation without the use of vibrating devices. Transition analysis of large-scale columns packed using the stop-flow method over multiple cycles has shown a two- to three-fold reduction of change in bed integrity values as compared to a flow-packed bed demonstrating an improvement in packed bed stability in terms of the height equivalent to a theoretical plate (HETP) and peak asymmetry (As).

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“…Recent advances in systems for preparative liquid chromatography enable the use of small-diameter particles, which increase separation performance [1,2,3,4]. Despite of the cost of this technique, it remains the best method for the purification of complex mixtures.…”

Section: Introductionmentioning

confidence: 99%

“…De Jong [2] has studied the influence of the packing and the injection system on peak tailing and proposed means of improving peak symmetry. Klawitzer [3], by using multiple sensors on the lower flange of a preparative column, deduced the shape of the sample zone inside the column in relation to the mode of injection used. Different types of injector have been reviewed by Gareil [4] who described the benefits and drawbacks of each system.…”

Section: Introductionmentioning

confidence: 99%

A new split-flow injector for preparative liquid chromatography columns. Annular injection system

2002

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The packing of large-diameter columns for liquid chromatography is still difficult and numerous publications have reported results from tests which prove the packing is heterogeneous. The slurry is more compact in the wall region and this reduces the flow of the mobile phase, leading to distortion of the sample zone in the column and generation of peak tailing. A new type of injection system for the head of the column has been developed which divides the flow of the solvent from the pump into two parts. One, without sample, is directed to a crown injector, close to the wall. By adjusting the ratio of this flow to that of the bulk flow it is possible to increase the speed of the mobile phase in this part of the stationary phase and reduce distortion of the sample zone. The other part of the solvent carries the sample to the stationary phase through a distributor. The results demonstrate the benefits of this annular injection system, which include increased efficiency and improved column stability.

show abstract

Investigation of the relationship between packing methods and efficiency of preparative columns

Cited by 22 publications

References 23 publications

The Column in Liquid Chromatography

The Column in Liquid Chromatography

Packing of large‐scale chromatography columns with irregularly shaped glass based resins using a stop‐flow method

A new split-flow injector for preparative liquid chromatography columns. Annular injection system

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