Investigation of the Lectin from the Edible Mushroom Agaricus bisporus as an Inhibitory Modulator of Healing after Trabeculectomy

Agaricus bisporus (Edible Mushroom Lectin) Inhibits Ocular Fibroblast Proliferation and Collagen Lattice Contraction

“…Hence experiments were designed to minimize the use of serum. In monolayer experiments, the minimum serum requirement for the maintenance of the viability of ®broblasts was 1 % (Tebbs et al, 2000).…”

“…After 3 days, monolayers were ®xed with 100 % methanol for 5 min, stained with haematoxylin for 3 min followed by immersion in running tap water for several minutes until cell nuclei appeared blue. Assuming that each cell contained only one nucleus a cell count was conducted using image analysis software (Aequitas IDA, Dynamic Data Links, Cambridge, U.K.) linked via a video camera to a Nikon Diaphot microscope as previously described (Tebbs et al, 2000). Monolayers were counted at 20 Â magni®cation.…”