Investigation of the Interaction of Herbal Ingredients Contained in Triphala Recipe Using Simplex Lattice Design: Chemical Analysis Point of View

Abstract

The aim of this work was to investigate the interaction of herbal ingredients contained in Triphala recipe (Terminalia chebula, Terminalia bellirica, and Phyllanthus emblica in equal proportion) using simplex lattice design. This work focused on chemical analysis of four phenolic compounds including gallic acid, corilagin, chebulagic acid, and chebulinic acid by validated high-performance liquid chromatography. The effect of the extraction technique (decoction vs. infusion) and gamma irradiation was also examined. The combination index was used as a tool for determination of interaction of the ingredients contained in the herbal recipe. Results showed that the extraction technique and gamma irradiation slightly altered the content of some phenolic compounds as well as the combination index. The positive interaction seems to be found at the equal proportion of the three plants. This work scientifically supported the suitable formula of the Triphala recipe in the traditional use.

Synergistic Antioxidant Activity and Optimal Microwave-Assisted Extraction Condition of Caesalpinia sappan L., Hibiscus sabdariffa L., and Clitoria ternatea L. Combinations

“…The previous works demonstrated the interaction of phenolic compounds contained in the herbal recipe, which might be related to the synergistic effect of the plant composed in the herbal recipe [33][34][35]. Therefore, this study introduced the evaluation of the synergistic antioxidant activity of herbal combinations used in herbal beverages.…”