Investigation of the genotoxic effect of microwave irradiation in rat bone marrow cells: in vivo exposure

Trošić, Ivančica; Bušljeta, Ivana; Modlic, Borivoj

doi:10.1093/mutage/geh042

Cited by 43 publications

(21 citation statements)

References 31 publications

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“…Our results indicated that TM value and BMN frequency were higher in an exposed population compare with a control group and the difference is significant. Recent in vitro and in vivo studies observed the occurrence of DNA damage (Zmyoelony et al 2000;Wolf et al 2005) as well as micronucleus (BMN) generation, which is a wellaccepted index for genotoxicity evaluation after RF-EMR exposure (Tice et al 2002;Trosic et al 2004). …”

Section: Discussionmentioning

confidence: 99%

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Gulati

Yadav

Kumar

et al. 2015

Arch Environ Contam Toxicol

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All over the world, people have been debating about associated health risks due to radiation from mobile phones and mobile towers. The carcinogenicity of this nonionizing radiation has been the greatest health concern associated with mobile towers exposure until recently. The objective of our study was to evaluate the genetic damage caused by radiation from mobile towers and to find an association between genetic polymorphism of GSTM1 and GSTT1 genes and DNA damage. In our study, 116 persons exposed to radiation from mobile towers and 106 control subjects were genotyped for polymorphisms in the GSTM1 and GSTT1 genes by multiplex polymerase chain reaction method. DNA damage in peripheral blood lymphocytes was determined using alkaline comet assay in terms of tail moment (TM) value and micronucleus assay in buccal cells (BMN). There was a significant increase in BMN frequency and TM value in exposed subjects (3.65 ± 2.44 and 6.63 ± 2.32) compared with control subjects (1.23 ± 0.97 and 0.26 ± 0.27). However, there was no association of GSTM1 and GSTT1 polymorphisms with the level of DNA damage in both exposed and control groups.

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Section: Discussionmentioning

confidence: 99%

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Gulati

Yadav

Kumar

et al. 2015

Arch Environ Contam Toxicol

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“…There is a concern about whether UHF-EMF irradiation is an agent that induces metabolic alterations that may trigger the development of a brain tumor [Salford et al, 2003;Lönn et al, 2004]. Some authors have claimed that the cancer risk is even more serious when it involves the irradiation of young subjects [Wertheimer and Leeper, 1979;Salford et al, 2003;Trosic et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress effects on the central nervous system of rats after acute exposure to ultra high frequency electromagnetic fields

Ferreira

Bonatto

Pasquali

et al. 2006

Bioelectromagnetics

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Mobile telephones and their base stations are an important source of ultra high frequency electromagnetic fields (UHF-EMFs; 800-1800 MHz) and their utilization is increasing all over the world. Epidemiological studies have suggested that low energy UHF-EMFs may have biological effects, such as changes in oxidative metabolism after exposure. Therefore, we have investigated the effect of acute UHF-EMF exposure on non-enzymatic antioxidant defense and lipid and protein oxidative damage in the rat frontal cortex and hippocampus. We have used malondialdehyde (MDA) and carbonyl assays to assess lipid and protein oxidative damages, respectively. No changes in lipid and protein damage, and also in non-enzymatic defense were found in frontal cortex or hippocampus. These results suggest that acute UHF-EMF exposure is not able to produce detectable oxidative stress in rats from any age tested. However, more tests using a longer period of exposure and evaluating other tissues are necessary to ensure that there is no health risk associated with the use of mobile phones.

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“…Considerable and reversible impact of RF/MW irradiation on proliferation and maturation of heamotopoietic cells was found in whole-body rats' exposure experiment (Trosic et al, 2004a). Although authors offer a credible mechanism of RF/MW action in vivo, we believe that precise insight into the mechanism would be accomplish by the in vitro approach to the problem.…”

Section: Discussionmentioning

confidence: 89%

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Pavičić

Trošić

2006

Biologia

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Considering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells continuous line V79. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) equipped by Philips PM 5508 signal generator cell samples were sub-cultivated for one day. Cell samples were exposed to 864 MHz continuous wave at an average specific absorption rate (SAR) of 0.08 W/kg. To determine cell growth, V79 cells were plated in concentration of 1 × 10 4 cells per milliliter of nutrient medium RPMI 1640, and raised in a humified atmosphere at 37• C in 5% CO2. Cell proliferation was determined by cell counts for each hour of exposure on post-exposure day 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/mL of RPMI 1640 and incubated according to the deliberated experimental protocol. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion test was used to determine viability of cells. In comparison to sham-exposed cells, growth curve of irradiated cell samples showed significant decrease (p < 0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from exposed "mock" condition. Under strictly controlled laboratory conditions, applied radiofrequency microwaves (RF/MW) irradiation significantly affected cell proliferation kinetics but not viability or ability of V79 cells to form colonies. Sophisticated mechanism of action is intending to be elucidated in the further research which will include insight into the RF/MW related event at the subcellular level.

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Investigation of the genotoxic effect of microwave irradiation in rat bone marrow cells: in vivo exposure

Cited by 43 publications

References 31 publications

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Oxidative stress effects on the central nervous system of rats after acute exposure to ultra high frequency electromagnetic fields

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

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