Investigation of the fluorescence spectra of chlorophylls a and b in ether solution

Studies on the Fluorescence of Chlorophyll. The Effects of Concentration, Temperature, and Solvent.

“…The major fluorescence maximum for component a in diethyl ether occurs at a considerably shorter wave length than that reported earlier by Zscheile (18), probably owing mostly to the occurrence of less autoabsorption during the present measurements. In the case of chlorophyll b, the central band reported earlier (18) was not found and was undoubtedly due to contamination of the component 6 preparation with component a, as found previously from absorption studies (20), and as discussed by Biermacher (1) in relation to his fluorescence studies and those by Dhéré and Raffy (4). Since the work of Biermacher ( 1) is so closely related to this study, several critical comparisons of methods and results should be made.…”

“…The measurements reported here were made with chlorophyll, prepared by methods previously described (10), of much greater purity than the material used formerly (18). The optical equipment and methods were also improved.…”