Studies on the Fluorescence of Chlorophyll. The Effects of Concentration, Temperature, and Solvent.

Zscheilc, F. P.; Harris, D. G.

doi:10.1021/j150432a001

Cited by 34 publications

(5 citation statements)

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“…Stokes, who discovered the basic principles of fluorescence, also found that chlorophyll fluoresces in leaves. In spite of the widespread occurrence in nature of chlorophyll and its scientific importance we know of only one investigation, Zscheile and Harris (32), reporting the precisely meastured fluorescence spectra of pure chlorophylls a and b.…”

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confidence: 99%

Fluorescence-Spectrum Curves of Chlorophylls, Pheophytins, Phycoerythrins, Phycocyanins and Hypericin.

et al. 1956

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Any quantitative investigations of fluorescence phenomena more or less presuppose some information as to the spectral emission curve of the substances under investigation. Knowledge of the shape of the slpectral emission curves of pure substances is necessary to various degrees of accuracy for the identification of pigments by fluorescence, for the detection of fluorescent impurities in mixtures, for the appropriate selection of filters or photocells to measuire the fluorescence intensity, and for planning experiiments so as to avoid reabsorption of the fluorescence within the sample. Reliable curves for the pure substances are particularly necessary for the analysis of the fluorescence spectra of complex fluorescent mixtures or of living cells. The fluorescent properties of chlorophyll have been investigated from many points of view since the fluorescence of chlorophyll was discovered by Brewster in 1834. Stokes, who discovered the basic principles of fluorescence, also found that chlorophyll fluoresces in leaves. In spite of the widespread occurrence in nature of chlorophyll and its scientific importance we know of only one investigation, Zscheile and Harris (32), reporting the precisely meastured fluorescence spectra of pure chlorophylls a and b.The fluorescence spectrum of bacteriochlorophyll in solution has been measured by Vermeulen, Wassink, and Reman (27) and in the intact bacteria by Duysens (9). The present paper revises slightly the calculations upon which Zscheile and Harris based their curves and presents precise curves for other plant pigrments, particularly those concerned in the process of photosynthesis.The yield of chlorophyll fluorescence in polar solvents has recently been found by Livingston and Forster (18) to be 25 %, in contrast to the older accepted value of 10 %. The low fluorescence in pure non-polar solvents and the enhancement of its intensity by traces of polar solvents has been studied by Watson, and McArdle (19 (13,14,15,23,29,30).Fluorescence spectroscopy of photosynthetic pigments in live plants has been used in studying energy transfer from one pigment to another

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Fluorescence-Spectrum Curves of Chlorophylls, Pheophytins, Phycoerythrins, Phycocyanins and Hypericin.

et al. 1956

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“…For example, a change in the diet from chlorophyll‐free to normal (with chlorophyll being present) can influence the spectra. Chlorophyll has both an absorption and a fluorescence peak in the 600 to 700 nm range . It was reported to interfere optical measurements in colon tissue and, thus, it is expected to be present in stool.…”

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Optical properties of mice's stool in 550 to 1000 nm wavelength range

Isler

Germanier

Ahnen

et al. 2017

Journal of Biophotonics

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The aim of this work was to measure optical properties of stool of mice to provide this relevant wavelength-dependent behavior for optical imaging modalities such as fluorescent molecular tomography and near-infrared optical tomography. BALB/c nude female mice were studied and optical properties of the stool were determined by employing the inverse adding-doubling approach. The animals were kept on chlorophyll-free diet. Nine stool samples were measured. The wavelength-dependent behavior of absorption and scattering in 550 to 1000 nm range is presented. The reduced scattering spectrum is fitted to the Mie scattering approximation in the near-infrared (NIR) wavelength range and to the Mie + Rayleigh approximation in visible/NIR range with the fitting coefficients presented. The study revealed that the absorption spectrum of stool can lead to crosstalk with the spectrum of hemoglobin in the NIR range.

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“…Vermeulen, Wassink, and Reman ( 1937 ) measured the fluorescence energy distribution curves of Clilorella, the purple bacterium Chromatium, a plant extract containing chlorophyll a and b and an extract of purple bacteria containing bacteriochlorophyll. Zscheile and Harris ( 1943 ) made precise measurements of the fluorescence spectra of pure chlorophylls a and b in various solvents. Van Norman, French, and Macdowall ( 1948 ) measured the fluorescence curves of two red marine algae and of a water extract from one of them.…”

Section: S -A-(xo -X)mentioning

confidence: 99%

The luminescence of biological systems; proceedings of the conference on luminescence, March 28-April 2, 1954, sponsored by the Committee on Photobiology of the National Academy of Sciences-National Research Council and supported by the National Science Foundation

Johnson¹

1955

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Prominent also are the studies led by A. J. Kluyver in the Netherlands, and by Yata Haneda and others in Japan. The luminescence of biological systems has now ripened into a fruitful area of general significance.Against this background, in brief, the Committee on Photobiology of the National Academy of Sciences-National Research Council, under the chairmanship of Sterling Hendricks and with the support of the National Science Foundation, recognized the mutual advantages of bringing together a group of leading investigators for a critical appraisal of present knowledge, for the first-hand interchange of experiences and ideas, and for the projection of likely approaches to unsolved problems. The conference that resulted was planned to include all aspects of bioluminescence, together with fundamental aspects of chemiluminescence and fluorescence. It was organized by a sub-committee composed of L.

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Studies on the Fluorescence of Chlorophyll. The Effects of Concentration, Temperature, and Solvent.

Cited by 34 publications

References 9 publications

Fluorescence-Spectrum Curves of Chlorophylls, Pheophytins, Phycoerythrins, Phycocyanins and Hypericin.

Fluorescence-Spectrum Curves of Chlorophylls, Pheophytins, Phycoerythrins, Phycocyanins and Hypericin.

Optical properties of mice's stool in 550 to 1000 nm wavelength range

The luminescence of biological systems; proceedings of the conference on luminescence, March 28-April 2, 1954, sponsored by the Committee on Photobiology of the National Academy of Sciences-National Research Council and supported by the National Science Foundation

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