Investigation of the effect of phlomisoside F on complete Freund's adjuvant-induced arthritis

Abstract

Abstract. Phlomis younghusbandii Mukerjee (Labiatae) has been reported to be effective in the treatment of rheumatoid arthritis (RA). In the present study, the anti-inflammatory and anti-arthritic effects of phlomisoside F (PF), isolated from P. younghusbandii Mukerjee (Labiatae), were investigated in male Wistar rats subjected to carrageen-induced paw edema and complete Freund's adjuvant (CFA)-induced arthritis. Arthritis scores were evaluated by a 5-point ordinal scale (scores 0-4). Expression levels of TNF-α, IL-1β, IL-6, IL-10, COX-2 and 5-LOX were determined via ELISA and western blot assays. Subsequent to establishing the edema and arthritis models, oral administration of PF (5, 10 and 20 mg/kg) significantly inhibited mean edema rate, compared with the control group in carrageenan-induced paw edema assay. In addition, administration of PF (5, 10 and 20 mg/kg/day) for 28 days markedly exhibited an anti-arthritic activity by offsetting the body weight loss, inhibiting the paw edema, reducing the arthritis scores and the indices of thymus and spleen, inhibiting the expression levels of TNF-α, IL-1β, IL-6, COX-2 and 5-LOX, and increasing the expression of IL-10, when compared with the respective control group in CFA-induced arthritis assay. In conclusion, PF is a valuable anti-arthritic constituent of P. younghusbandii, and the present study results suggest that this herb may be used in the treatment of RA.

Therapeutic benefits of Indole-3-Carbinol in adjuvant-induced arthritis and its protective effect against methotrexate induced-hepatic toxicity

“…Even the intensity of liver injury was revealed at histological level with increased inflammatory infiltration. These results were in accordance with the results of other studies that have investigated the action of CFA-induced arthritis in rats [7, 20, 24, 41–43].…”

Anti-arthritic activity of D-carvone against complete Freund’s adjuvant-induced arthritis in rats through modulation of inflammatory cytokines

“…CFA is known to induce chronic inflammation by up-regulating the migration of lymphocytes and macrophages along with the release of inflammatory mediators such as TNF-α, IL-6, IL-1β, and IL-10 [ 15 , 18 ]. These mediators in turn, cause damage to the tissue and articular cartilages, leading to osteoarthritic conditions [ 19 ]. These changes were observed in the results of the CFA-induced model rats, with inflammatory cell infiltration in histopathological results and elevation of inflammatory mediators in ELISA analysis.…”

Evaluation of antiarthritic activity of nimbolide against Freund’s adjuvant induced arthritis in rats

“…Besides these changes, animals induced with arthritis was also found to show high arthritic index score and paw swelling [39]. Paw swelling indicates the measure of intensity of inflammation in CFA induced rats and reduction in paw swelling following treatment indicates the antiarthritic activity of the drug [40].…”