Investigation of the composition and antibacterial activity of Ukrain™ drug using liquid chromatography techniques

Jesionek, Wioleta; Fornal, Emilia; Majer‐Dziedzic, Barbara; Móricz, Ágnes M.; Nowicky, Wassil; Choma, Irena

doi:10.1016/j.chroma.2015.12.015

Cited by 21 publications

(14 citation statements)

References 23 publications

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“…The MS and NMR spectra of compounds 1-3 were in complete agreement with the literature data of stylopine, corydaline, and dehydrocorydaline ( Fig. 1), respectively [15][16][17][18][19][20]. The C NMR spectra are summarized in Table S1.…”

supporting

confidence: 83%

In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

Han

Shim

Jang

et al. 2018

Journal of Microbiology and Biotechnology

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Phytochemicals have been considered as alternatives for synthetic fungicides because of their biodegradability and low toxicity. In this study, we found that the methanolic extract of suppressed the development of plant diseases caused by and . As the antifungal substance, three isoquinoline alkaloids (dehydrocorydaline, stylopine, and corydaline) were isolated from. These active compounds also exhibited in vivo antifungal activity against and. Taken together, our results suggest that and its active compounds can be used to control plant diseases.

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supporting

confidence: 83%

In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

Han

Shim

Jang

et al. 2018

Journal of Microbiology and Biotechnology

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“…Methanol or ethanol often with addition of water (Bugatti et al, 1987 ; Han et al, 1991 ; Koriem et al, 2013 ) and hydrochloric (Kursinszki et al, 2006 ; Gu et al, 2010 ) or acetic acid (Paulsen et al, 2015 ) were used as extractants. The isolation from plant material was also achieved with the use of pure acidified water; further, the solution was alkalized with ammonia or sodium hydroxide to obtain base forms followed by liquid-liquid extraction with organic solvents such as dichloromethane, butanol or chloroform (Sārközi et al, 2006 ; Sárközi et al, 2007 ; Migas et al, 2012 ; Jesionek et al, 2016 ; Bogucka-Kocka and Zalewski, 2017 ). The isolation was conducted by percolation (Capistrano et al, 2015 ), maceration (Koriem et al, 2013 ; Borghini et al, 2015 ), heating under reflux (Gu et al, 2010 ; Yao et al, 2011 ; Seidler-Łozykowska et al, 2016 ), in water bath (Sārközi et al, 2006 ) or Soxhlet apparatus (Bugatti et al, 1987 ; Stuppner and Ganzera, 1995 ) as well as ultrasound assisted (UAE) (Kursinszki et al, 2006 ; Sárközi et al, 2007 ; Paulsen et al, 2015 ; Jesionek et al, 2016 ) or microwave energy (MAE) (Then et al, 2000 ; Zhou et al, 2012 ).…”

Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

“…Silica was the most common stationary phase, often impregnated with salts (Ni, Zn, Cr, Co) to improve the selectivity (Wagner et al, 1984 ; Then et al, 2000 ; Waksmundzka-Hajnos et al, 2000 ; Sārközi et al, 2006 ; Sárközi et al, 2007 ; Petruczynik et al, 2008 ; Jesionek et al, 2016 ) Silica modified with octadecyl (C-18) and cyanopropyl (CN) groups and aqueous solutions of methanol or isopropanol with ammonia or diethylamine which prevented tailing of chromatographic bands were sporadically applied (Petruczynik et al, 2007 ). Petruczynik et al ( 2008 ) combined various types of stationary phases e.g., cyanopropyl silica with silica or octadecyl silica to obtain adsorbent gradient.…”

Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

“…TLC was also applied for direct bioautography (TLC-DB) to test the antibacterial activity of C. majus extracts (Sárközi et al, 2007 ; Móricz et al, 2015 ; Jesionek et al, 2016 ) combined with densitometry for quantitative analysis (Then et al, 2000 ; Sārközi et al, 2006 ) and it proved useful as preparative technique for isolation of alkaloids (Waksmundzka-Hajnos et al, 2002 ; Koriem et al, 2013 ).…”

Section: Methods For Analysis Of Active Components From Chementioning

confidence: 99%

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Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

et al. 2018

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As antique as Dioscorides era are the first records on using Chelidonium as a remedy to several sicknesses. Inspired by the “signatura rerum” principle and an apparent ancient folk tradition, various indications were given, such as anti-jaundice and cholagogue, pain-relieving, and quite often mentioned—ophthalmological problems. Central and Eastern European folk medicine has always been using this herb extensively. In this region, the plant is known under many unique vernacular names, especially in Slavonic languages, associated or not with old Greek relation to “chelidon”—the swallow. Typically for Papaveroidae subfamily, yellow-colored latex is produced in abundance and leaks intensely upon injury. Major pharmacologically relevant components, most of which were first isolated over a century ago, are isoquinoline alkaloids—berberine, chelerythrine, chelidonine, coptisine, sanguinarine. Modern pharmacology took interest in this herb but it has not ended up in gaining an officially approved and evidence-based herbal medicine status. On the contrary, the number of relevant studies and publications tended to drop. Recently, some controversial reports and sometimes insufficiently proven studies appeared, suggesting anticancer properties. Anticancer potential was in line with anecdotical knowledge spread in East European countries, however, in the absence of directly-acting cytostatic compounds, some other mechanisms might be involved. Other properties that could boost the interest in this herb are antimicrobial and antiviral activities. Being a common synanthropic weed or ruderal plant, C. majus spreads in all temperate Eurasia and acclimates well to North America. Little is known about the natural variation of bioactive metabolites, including several aforementioned isoquinoline alkaloids. In this review, we put together older and recent literature data on phytochemistry, pharmacology, and clinical studies on C. majus aiming at a critical evaluation of state-of-the-art from the viewpoint of historical and folk indications. The controversies around this herb, the safety and drug quality issues and a prospective role in phytotherapy are discussed as well.

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“…Because of the broad spectrum of action, C. majus alkaloids are a subject of interest for pharmacology and toxicology; therefore, the effective analytical methods for their investigation are still being developed. Spectrophotometry [ 5 , 6 ], capillary electrophoresis [ 7 , 8 ], and thin-layer chromatography [ 9 – 12 ] have been used for this purpose; however, high-performance liquid chromatography is the most common for qualitative and quantitative analyses of C. majus [ 5 , 13 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

Sowa

Zielińska

Sawicki

et al. 2018

Journal of Analytical Methods in Chemistry

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Chelidonium majus L. is a rich source of isoquinoline alkaloids with confirmed anti-inflammatory, choleretic, spasmolytic, antitumor, and antimicrobial activities. However, their chromatographic analysis is difficult because they may exist both in charged and uncharged forms and may result in the irregular peak shape and the decrease in chromatographic system efficacy. In the present work, the separation of main C. majus alkaloids was optimized using a new-generation XB-C18 endcapped core-shell column dedicated for analysis of alkaline compounds. The influence of organic modifier concentration, addition of salts, and pH of eluents on chromatographic parameters such as retention, resolution, chromatographic plate numbers, and peak asymmetry was investigated. The results were applied to elaborate the optimal chromatographic system for simultaneous quantification of seven alkaloids from the root, herb, and fruit of C. majus.

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Investigation of the composition and antibacterial activity of Ukrain™ drug using liquid chromatography techniques

Cited by 21 publications

References 23 publications

In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

In Vivo Disease Control Efficacy of Isoquinoline Alkaloids Isolated from Corydalis ternata against Wheat Leaf Rust and Pepper Anthracnose

Greater Celandine's Ups and Downs−21 Centuries of Medicinal Uses of Chelidonium majus From the Viewpoint of Today's Pharmacology

Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

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