Investigation of the Chemical Composition and Different Effects of a Rumex dentatus Methanol Extract Against Drug Resistant Pseudomonas aeruginosa Isolates

Abstract

Background: The emergence of Pseudomonas aeruginosa as a multiple drug resistant bacteria has renewed efforts to identify alternative antibacterial materials, such as medicinal plants. Objectives:The aims of this study were to investigate the antibacterial effects of a Rumex dentatus methanol extract against drug resistant P. aeruginosa isolates and to analyze the chemical composition of this extract. Materials and Methods: Sixty strains of P. aeruginosa were isolated from urine and wound samples and identified using conventional tests, as well as 16S rDNA gene sequencing. Results: The isolates were completely resistant to the tested antibiotics, with minimum inhibitory concentration (MIC) values for

Antibiofilm Activity of Methanol Extract of Rumex dentatus Against Pseudomonas aeruginosa

“…PCR was carried out in 25 μl reaction mixture consisting of 2.5 μl 10x pcr buffer, 2 μl of dNTP mix (concentration 10 mM)(Fermentase, Germany), 5 μl of MgCl2 (concentration 25 mM), primer forward and reverse 0.5 μl (concentration 10 mM), 0.2 μl Taq DNA polymerase (Genetbio, Korea), 2 μl of DNA (150-200 ng) and 12.3 μl nuclease free water. In our previous study (Pezeshki Najafabadi et al, 2016), in accordance with this one, PCR results and images were presented.…”

Anti-Quorum Sensing and Antibacterial Activity of Rumex alveolatus

“…Studies have indicated that polyphenols including tannins, anthrakinones, and flavonoids have effective antimicrobial effects against various microorganisms in addition to their antioxidant properties [21,22]. Therefore, considering the presence of anthraquinones and flavonoids in plants of the Polygonaceae family, antimicrobial effects of methanol extracts of R. alveolatus leaves and roots can be attributed to these compounds [23][24][25][26][27].…”