Investigation of the batch-to-batch inconsistencies of Collagen in PCL-Collagen nanofibers

Dippold, Dirk; Cai, Aijia; Hardt, Moritz; Boccaccını, Aldo R.; Horch, Raymund E.; Beier, Justus P.; Schubert, Dirk W.

doi:10.1016/j.msec.2018.10.057

Cited by 32 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduced reproducibility of natural materials might be explained by the effect of batch-to-batch variability typical of naturally derived sources. When there is a variation in the material used, even from the same brand, this can cause some errors when performing the same bioprinting process [ 42 ]. Because synthetic polymers have a low contribution (28.37%) in the literature reviewed, the different types of polymers have been organized according to their source or origin.…”

Section: Methodsmentioning

confidence: 99%

Cancer Cell Direct Bioprinting: A Focused Review

et al. 2021

View full text Add to dashboard Cite

Three-dimensional printing technologies allow for the fabrication of complex parts with accurate geometry and less production time. When applied to biomedical applications, two different approaches, known as direct or indirect bioprinting, may be performed. The classical way is to print a support structure, the scaffold, and then culture the cells. Due to the low efficiency of this method, direct bioprinting has been proposed, with or without the use of scaffolds. Scaffolds are the most common technology to culture cells, but bioassembly of cells may be an interesting methodology to mimic the native microenvironment, the extracellular matrix, where the cells interact between themselves. The purpose of this review is to give an updated report about the materials, the bioprinting technologies, and the cells used in cancer research for breast, brain, lung, liver, reproductive, gastric, skin, and bladder associated cancers, to help the development of possible treatments to lower the mortality rates, increasing the effectiveness of guided therapies. This work introduces direct bioprinting to be considered as a key factor above the main tissue engineering technologies.

show abstract

Section: Methodsmentioning

confidence: 99%

Cancer Cell Direct Bioprinting: A Focused Review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Collagen is the most abundant protein in the human body and has become one of the most widely used materials in tissue engineering due to its superior biological properties [ 1 ]. Compared with land-based collagen, collagen derived from aquatic animals has the advantages of a wide range of sources, low production cost, low immunogenicity, and is free from the influence of mad cow disease, religion, and other factors [ 2 ], so it has been applied more and more widely in the field of materials.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene Oxide Assisted Fish Collagen-Poly-ε-Caprolactone Nanofiber Membranes by Electrospinning

Wang

et al. 2022

Nanomaterials

View full text Add to dashboard Cite

Fish collagen has higher biocompatibility and lower immunogenicity than terrestrial collagen, and is currently one of the important raw materials for preparing biological materials. In this study, PEO was used as a spinning aid to prepare fish skin collagen-PCL nanofiber membranes by electrospinning, and the process was optimized to get smooth nanofibers. The morphological and mechanical properties of collagen-PCL nanofiber membranes were assessed by scanning electron microscopy (SEM). The changes in chemical composition due to the incorporation of collagen into PCL and PEO were determined by Fourier Transform infrared spectroscopy (FTIR). The biocompatibility of the collagen-PCL nanofiber membranes was evaluated in vitro in cultures of mouse fibroblasts and in vivo by subcutaneous implantation studies in rats. It was found that the diameter of the spun fibers became fine and smooth when the ratio of the collagen/PCL increased. The finally obtained nanofiber had good mechanical strength, porosity, and hydrophilicity, and could promote cell adhesion and proliferation. The FC-PCL nanofiber membrane prepared by this route opens a new way to prepare fish collagen biomaterials with electrospinning.

show abstract

“…While natural hydrogels such as protein gels usually fulfill the bio-related requirements, their weak mechanical properties as well as the batch-to-batch variations resulting from their animal-based origins limit the application scope. [5,6] In contrast, the chemical and mechanical properties of synthetic hydrogels are by far easier to adjust to the application specific demands. However, synthetic materials are usually bioinert, and cell-adhesion must be enabled through additional bio-linkers or coatings that allow interactions with cell surface receptors.…”

Section: Introductionmentioning

confidence: 99%

Cell Adhesion on UV‐Crosslinked Polyurethane Gels with Adjustable Mechanical Strength and Thermoresponsiveness

Breul

Stengelin

Urschbach

et al. 2021

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Temperature-responsive polyurethane (PU) hydrogels represent a versatile material platform for modern tissue engineering and biomedical applications. However, besides intrinsic advantages such as high mechanical strength and a hydrolysable backbone composition, plain PU materials are generally lacking bio-adhesive properties. To overcome this shortcoming, the authors focus on the synthesis of thermoresponsive PU hydrogels with variable mechanical and cell adhesive properties obtained from linear precursor PUs based on poly(ethylene glycol)s (pEG) with different molar masses, isophorone diisocyanate, and a dimerizable dimethylmaleimide (DMMI)-diol. The cloud point temperatures of the dilute, aqueous PU solutions depend linearly on the amphiphilic balance. Rheological gelation experiments under UV-irradiation reveal the dependence of the gelation time on photosensitizer concentration and light intensity, while the finally obtained gel strength is determined by the polymer concentration and spacing of the crosslinks. The swelling ratios of these soft hydrogels show significant changes between 5 and 40 °C whereby the extent of this switch increases with the hydrophobicity of the precursor. Moreover, it is shown that the incorporation of a low amount of catechol groups into the networks through the DMMI dimerization reaction leads to strongly improved cell adhesive properties without significantly weakening the gels.

show abstract

Investigation of the batch-to-batch inconsistencies of Collagen in PCL-Collagen nanofibers

Cited by 32 publications

References 28 publications

Cancer Cell Direct Bioprinting: A Focused Review

Cancer Cell Direct Bioprinting: A Focused Review

Polyethylene Oxide Assisted Fish Collagen-Poly-ε-Caprolactone Nanofiber Membranes by Electrospinning

Cell Adhesion on UV‐Crosslinked Polyurethane Gels with Adjustable Mechanical Strength and Thermoresponsiveness

Contact Info

Product

Resources

About