Investigation of Preeclampsia Using Raman Spectroscopy

Abstract

Preeclampsia is associated with increased perinatal morbidity and mortality. There have been numerous efforts to determine preeclampsia biomarkers by means of biophysical, biochemical, and spectroscopic methods. In this study, the preeclampsia and control groups were compared via band component analysis and multivariate analysis using Raman spectroscopy as an alternative technique. The Raman spectra of serum samples were taken from nine preeclamptic, ten healthy pregnant women. The Band component analysis and principal component analysis-linear discriminant analysis were applied to all spectra after a sensitive preprocess step. Using linear discriminant analysis, it was found that Raman spectroscopy has a sensitivity of 78% and a specificity of 90% for the diagnosis of preeclampsia. Via the band component analysis, a significant difference in the spectra of preeclamptic patients was observed when compared to the control group. 19 Raman bands exhibited significant differences in intensity, while 11 of them decreased and eight of them increased. This difference seen in vibrational bands may be used in further studies to clarify the pathophysiology of preeclampsia.

Raman spectroscopy as a novel method in placental research: Recognizing the pattern of placental hypoxia

“…The hypoxic treatment of placenta ex vivo in our study represents the models, mimicking pre-eclampsia (PE) [10a] ; interestingly, these spectra are similar to the Raman shift, reported for human normal and pre-eclamptic placentas [8] and blood samples from women with PE. [13] The Raman peak of 1,129 cm −1 was quite close to 1,127 cm −1 hemoglobin-derived hemoporfirin shift reported in exposed to hypoxia erythrocytes and myocardium [6,14] and the same as found in placentas from women suffering from PE. [8] 1,128 cm −1 Raman shift was identified for myristic acid, which has also chain vibration expansion shift of 414 cm −1 [15] -close to detected in our study 415 cm −1 .…”

Embryo viability indexing using Raman spectroscopy of spent culture media

“…Fig. 1 and is similar to our previous studies [27,28]. In The sample container, shown in the insert of Fig.…”

“…Several diseases such as cardiovascular diseases [24], cancer [25], and blood diseases [26] were also studied with Raman spectroscopy. Our group [27] has already searched for evidence of preeclampsia at a molecular level using Raman spectra of serum samples, which were taken from both healthy and pregnant women with preeclampsia.…”

Raman spectroscopy of blood serum for Alzheimer's disease diagnostics: specificity relative to other types of dementia

“…In this regard, the diagnostic potential of Raman spectroscopy has recently been demonstrated for several diseases . Raman spectroscopy has been applied to diagnose different types of cancer, diabetes, atherosclerosis, Alzheimer's and Parkinson's disease .…”