Investigation of postpartum dairy cows’ uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene

Machado, V.S.; Oikonomou, Georgios; Bicalho, M.L.S.; Knauer, W. A.; Gilbert, R.O.; Bicalho, R.C.

doi:10.1016/j.vetmic.2012.04.033

Cited by 133 publications

(135 citation statements)

References 25 publications

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“…Members of the genus Helcococcus, found to be associated with metritis in this study, were also associated with endometritis in the study by Machado et al (8). Herein, Helcococcus was positively correlated with the uterine discharge score, and Helcococcus ovis was identified as the most prevalent species for this genus.…”

Section: Discussionsupporting

confidence: 72%

“…Therefore, further investigation of the role of Helcococcus and Filifactor and the main species from these genera in the development of metritis is warranted. Peptoniphilus was also associated with endometritis in the study by Machado et al (8). The genus Prevotella was positively correlated with bacteria considered important, such as Porphyromonas, which indicates an indirect association with metritis.…”

Section: Discussionmentioning

confidence: 82%

“…One study using pyrosequencing observed a predominance of Bacteroidetes and Fusobacteria in both metritic and healthy cows (7). One study using pyrosequencing observed higher abundances of Bacteroidetes, Tenericutes, and Fusobacteria in cows with endometritis than in healthy cows (8). A recent study using MiSeq sequencing found a predominance of the families Fusobacteriaceae, Bacteroidaceae, Porphyromonadaceae, and Pasteurellaceae in the uterine fluid of cows with pyometra (17).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, cultureindependent techniques such as clone library sequencing (5,6) and pyrosequencing (7,8) have been used to characterize the uterine microbiota of cows with metritis (5-7) and endometritis (7,8). Sequencing using the Illumina platform allows for deeper sequencing than has previously been feasible even with pyrosequencing (9).…”

mentioning

confidence: 99%

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Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows

Jeon

Vieira-Neto

Gobikrushanth

et al. 2015

Appl Environ Microbiol

145

174

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The objective of this study was to evaluate the progression of the uterine microbiota from calving until establishment of metritis. Uterine swabs (n ‫؍‬ 72) collected at 0, 2, and 6 ؎ 2 days postpartum (dpp) from 12 metritic and 12 healthy cows were used for metagenomic sequencing of the 16S rRNA gene on the Illumina MiSeq platform. A heat map showed that uterine microbiota was established at calving. The microbiota changed rapidly from 0 to 6 ؎ 2 dpp, with a decrease in the abundance of Proteobacteria and an increase in the abundance of Bacteroidetes and Fusobacteria, which were dominant in metritic cows. Uterine microbiota composition was shared; however, metritic and healthy cows could be discriminated using relative abundance of bacterial genera at 0, 2, and 6 ؎ 2 dpp. Bacteroides was the main genus associated with metritis because it was the only genus that showed significantly greater abundance in cows with metritis. As the abundance of Bacteroides organisms increased, the uterine discharge score, a measure of uterine health, worsened. Fusobacterium was also an important genus associated with metritis because Fusobacterium abundance increased as Bacteroides abundance increased and the uterine discharge score worsened as the abundance increased. The correlation with uterine discharge score and the correlation with Bacteroides or Fusobacterium showed that other bacteria, such as Helcoccocus, Filifactor, and Porphyromonas, were also associated with metritis. There were also bacteria associated with uterine health, such as "Candidatus Blochmannia," Escherichia, Sneathia, and Pedobacter. Metritis is a huge concern for the dairy industry worldwide because it is highly prevalent (25 to 40%) and negatively affects the productivity, survival, and welfare of dairy cows (1). Diverse bacteria, including anaerobes and facultative anaerobes, were observed in the uteri of dairy cows within the first 2 weeks postpartum, but they were naturally cleared out within 60 days postpartum (dpp) (1). Culture-based studies observed that Escherichia coli, Trueperella pyogenes, Fusobacterium necrophorum, and Bacteroides spp. (e.g., Prevotella melaninogenica, formerly Bacteroides melaninogenicus) were commonly associated with endometritis or pyometra (1-3).Although culture-based studies have laid out the foundation of our understanding of the uterine microbiota, previous studies might have underestimated the microbial complexity of the intrauterine environment of cows postpartum, given that less than 1% of the microorganisms in many environments are readily cultured under standard laboratory conditions (4). In recent years, cultureindependent techniques such as clone library sequencing (5, 6) and pyrosequencing (7, 8) have been used to characterize the uterine microbiota of cows with metritis (5-7) and endometritis (7,8). Sequencing using the Illumina platform allows for deeper sequencing than has previously been feasible even with pyrosequencing (9). Indeed, evaluating the rarefaction curves from previous 16S rRNA sequencing studies ...

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows

Jeon

Vieira-Neto

Gobikrushanth

et al. 2015

Appl Environ Microbiol

145

174

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“…In addition, vaccines containing components of E. coli, F. necrophorum and/or T. pyogenes protect animals against postpartum uterine disease (Nolte et al, 2001;Machado et al, 2014). However, metagenomics techniques have found associations between uterine disease and bacteria that are not readily cultured by standard techniques (Machado et al, 2012;Santos and Bicalho, 2012;Peng et al, 2013;Knudsen et al, 2015;Wagener et al, 2015). Whilst some of the studies find E. coli, T. pyogenes and the expected anaerobic bacteria, others report finding Bacteroidetes and Firmicutes.…”

Section: Microbes That Cause Uterine Diseasementioning

confidence: 99%

Postpartum uterine infection and endometritis in dairy cattle

Sheldon¹,

Owens²

2017

Anim Reprod

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After parturition, uterine involution, regeneration of the endometrium, return of ovarian cyclic activity, and the control of pathogenic bacteria in the uterus is required before cows are likely to conceive again. However, pathogenic bacteria often cause uterine disease in modern dairy cattle, leading to decreased productivity and reduced fertility. This review aims to provide an overview of postpartum uterine infection and disease in dairy cattle. Metritis and endometritis are the main postpartum clinical conditions; although, subclinical endometritis is an emerging issue. Postpartum uterine disease is associated with the isolation of Escherichia coli, Trueperella pyogenes, and anaerobic pathogenic bacteria. Sensing of bacteria or their pathogen-associated molecules, such as lipopolysaccharide, by the innate immune system generates inflammatory responses. Endometrial inflammation includes increased expression of complement, calgranulins, interleukins and acute phase proteins, as well as the chemotaxis of neutrophils and macrophages to the site of infection. Uterine disease is also characterised by tissue damage, including endometrial cytolysis caused by the cholesteroldependent cytolysin, pyolysin. The responses to pathogens are energetically expensive, and depletion of the key cellular nutrients, glucose or glutamine, impairs inflammatory responses by endometrial tissues. For sustainable intensification of the dairy industry over the next 50 years, it is vital to understand why high-milkyield cows are so susceptible to uterine pathology and develop new ways to prevent uterine disease.

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Interaction of Nutrition and Reproduction in the Dairy Cow

Cargile¹,

Tracy

2014

Bovine Reproduction

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Investigation of postpartum dairy cows’ uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene

Cited by 133 publications

References 25 publications

Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows

Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows

Postpartum uterine infection and endometritis in dairy cattle

Interaction of Nutrition and Reproduction in the Dairy Cow

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