Investigation of plant latices of Asteraceae and Campanulaceae regarding proteolytic activity

Sytwala, S; Domsalla, A; Melzig, Matthias F.

doi:10.1016/j.plaphy.2015.09.017

Cited by 3 publications

(3 citation statements)

References 49 publications

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“…Interestingly, majority of latices screened in the present study contains either cysteine or serine proteases except for few which showed inhibition for more than one type of protease inhibitors suggesting the presence of a mixture of protease types or involvement of more than one amino acid in the active site (Table ). The presence of the mixture of proteases in the same latex has been reported in the Asteraceae and Campanulaceae latices …”

Section: Resultssupporting

confidence: 66%

“…The presence of the mixture of proteases in the same latex has been reported in the Asteraceae and Campanulaceae latices. 47 Latex protease fractions (LPFs) were screened for procoagulant activity as latices are extensively used by folk healers for treating bleeding wounds. All the LPFs reduced platelet poor plasma clotting time suggesting their procoagulant nature (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

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Plant latex thrombin‐like cysteine proteases alleviates bleeding by bypassing factor VIII in murine model

Urs

Rudresha

Manjuprasanna

et al. 2019

J of Cellular Biochemistry

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Hemostasis is a tightly regulated process which maintains a fluid state of blood within the vasculature and provides thrombotic response upon tissue injury. Various scientific studies have implicated the role of plant latex proteases in hemostasis using in vitro experiments. However, in vivo models substantiate their role in hemostasis. Therefore, in the present study, the effect of plant latex thrombin-like proteases (PTLPs) on hemostasis was investigated systematically using mice tail bleeding as a preclinical model. In this direction, latex protease fractions (LPFs), which showed potent thrombin-like activity, were selected as they act directly on fibrinogen to form clot and quickly stop bleeding.Thrombin-like activity was exhibited mainly by cysteine proteases. Calotropis gigantea, Carica papaya, Jatropha curcas, Oxystelma esculentum, Tabernaemontana divaricata, and Vallaris solanacea LPFs and papain from C. papaya latex significantly reduced bleeding on a topical application in normal and aspirin administered mice. In addition, PTLPs accelerated the clotting of factor VIII deficient plasma, while, papain brought back the clotting time to normal levels acting like a bypassing agent. Further, papain failed to show activity in the presence of specific cysteine protease inhibitor iodoacetic acid; confirming protease role in all the activities exhibited. At the tested dose, PTLPs except C. gigantea did not show toxicity. Further, structural and sequence comparison between PTLPs and human thrombin revealed structural and sequence dissimilarity indicating their unique nature. The findings of the present study may open up a new avenue for considering PTLPs including papain in the treatment of bleeding wounds. K E Y W O R D S bypassing agent, hemophilia, hemostasis, intrinsic pathway, papain, procoagulant protease

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Plant latex thrombin‐like cysteine proteases alleviates bleeding by bypassing factor VIII in murine model

Urs

Rudresha

Manjuprasanna

et al. 2019

J of Cellular Biochemistry

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“…88,90,93 Among higher plants Apocyanaceae, Euphorbiaceae, Moraceae, Papaveraceae, Caricaceae are major families containing a high amount of these proteases. 94 It is interesting that a lot of plant-based folk medicine dwells on these proteases. Food allergens cause sensitisation towards different proteins.…”

Section: Plantsmentioning

confidence: 99%

A critical review on serine protease: Key immune manipulator and pathology mediator

Patel

2017

Allergologia et Immunopathologia

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Proteolytic activity is fundamental to survival, so it is not surprising that all living organisms have proteases, especially seine protease. This enzyme in its numerous isoforms and homologues, constitutes the quintessential offence and defence factors, in the form of surface proteins, secreted molecules, gut digestive enzymes, venom in specialised glands or plant latex, among other manifestations. Occurring as trypsin, chymotrypsin, elastase, collagenase, thrombin, subtilisin etc., it mediates a diverse array of functions, including pathological roles as inflammatory, coagulatory to haemorrhagic. This review emphasizes that despite the superficial differences in mechanisms, most health issues, be they infectious, allergic, metabolic, or neural have a common conduit. This enzyme, in its various glycosylated forms leads to signal misinterpretations, wreaking havoc. However, organisms are endowed with serine protease inhibitors which might restrain this ubiquitous yet deleterious enzyme. Hence, serine proteases-driven pathogenesis and antagonising role of inhibitors is the focal point of this critical review.

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Antibacterial cysteine protease from Cissus quadrangularis L.

Muthu

Gopal

Karthik

et al. 2017

International Journal of Biological Macromolecules

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Investigation of plant latices of Asteraceae and Campanulaceae regarding proteolytic activity

Cited by 3 publications

References 49 publications

Plant latex thrombin‐like cysteine proteases alleviates bleeding by bypassing factor VIII in murine model

Plant latex thrombin‐like cysteine proteases alleviates bleeding by bypassing factor VIII in murine model

A critical review on serine protease: Key immune manipulator and pathology mediator

Antibacterial cysteine protease from Cissus quadrangularis L.

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