Antibacterial cysteine protease from  Cissus quadrangularis  L.

Muthu, Sakthivel; Gopal, Venkatesh Babu; Karthik, Sankar Narayan; Sivaji, Prabu; Sathuvan, Malairaj; Lakshmikanthan, Mythileeswari; Subramani, Nagaraj; Palani, P.

doi:10.1016/j.ijbiomac.2017.05.107

Cited by 14 publications

(6 citation statements)

References 47 publications

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“…Its use in wound healing can be attributed to its reported anti-infammatory, analgesic, and antimicrobial properties. Te plant is known to contain bioactive compounds such as favonoids, terpenoids, alkaloids, and other phytochemicals, which may contribute to its therapeutic efects [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Ethnoveterinary Study of Plant‐Based Remedies for Treating Diseases in Small Ruminants in Maputo Province, Mozambique

Barbosa,

Cala,

Sevastyanov

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Small ruminants, particularly goats and sheep, are key livestock species in Mozambique, and their production is mainly undertaken by families. However, small ruminants are often plagued by diseases that can cause considerable economic damage. In this context, traditional remedies, including various plant species, have been widely used to manage these diseases. The aim of this study was to explore the use of plant-based remedies and other treatments for managing diseases in small ruminants in Maputo Province, Mozambique. Data collection involved conducting interviews with 44 small ruminant breeders across 5 districts in Maputo Province to identify the plants and other remedies commonly used for managing diseases in their animals. We identified a total of 38 plant species belonging to 22 families. Among these plants, four were not identified by their scientific name. The most important plants reported were Cissus quadrangularis, Euphorbia kirkii, and Aloe sp., with Cissus quadrangularis being particularly noteworthy as it was frequently cited for the treatment of wounds. The most commonly cited botanical families were Fabaceae, Euphorbiaceae, and Asphodelaceae. Interestingly, in addition to plant-based remedies, we also identified other nonplant sources of treatment, such as alkaline batteries, which are commonly used to treat wounds in small ruminants. The use of plants for ethnoveterinary purposes remains prevalent in Maputo province, with older breeders serving as the primary custodians of this traditional knowledge. Efforts should be made to document and share the knowledge of these older breeders, ensuring that it is not lost over time. This preservation of ethnoveterinary knowledge can contribute to sustainable livestock management and support the wellbeing of both rural communities and their animals.

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Section: Discussionmentioning

confidence: 99%

Ethnoveterinary Study of Plant‐Based Remedies for Treating Diseases in Small Ruminants in Maputo Province, Mozambique

Barbosa,

Cala,

Sevastyanov

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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“…In general, proteases from medicinal plants are preferred for industrial application as they do not require any co-factors (Asif-Ullah et al, 2006). Cysteine proteases were purified from C. quadrangularis by Muthu et al (2017), and the safety of this plant in bone fracture treatment, including enhanced bone growth and anti-osteoporotic activities, were reported by Sawangjit et al (2017).…”

Section: Resultsmentioning

confidence: 99%

In vitro anti-inflammatory, anti-diabetic and antioxidant potential of Cissus quadrangularis along with its orexigenic activity in Drosophila melanogaster

Zaki¹,

L²,

Bansal³

et al. 2021

JANS

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Plants with diverse pharmacological activities are actively being explored for human health. Cissus quadrangularis (L) has been reported to possess numerous phytochemicals and is used to relieve various disorders. This article aims at providing evidence of the diverse pharmacological activities in terms of orexigenic, anti-inflammatory, anti-diabetic and antioxidant activities of C. quadrangularis for further application in clinical development. The results revealed that inhibition of hemolysis was within the range of 8-9-25.6% at concentrations of 12.5-200 µg/ml. Methanol extract of C. quadrangularis stems exhibited porcine pancreatic ?-amylase (PPA) inhibition (p?0.05) at concentrations of 0.25 and 0.30 mg/ml. The glucose adsorption capacity of the C. quadrangularis was observed to be inversely proportional to the molar concentration of glucose. The higher food intake by Drosophila in food medium with plant extract is presumably related to orexigenic property of C. quadrangularis. Protease activity of C. quadrangularis stem extract revealed total activity 975 U/ml and specific activity as 3768 U/mg. The absorbance of C. quadrangularis in reducing power assay were between 0.91and 1.85. Highest total antioxidant activity of 67.2 µg TE/g was observed and the hydroxyl radicals scavenging activity was observed in a dose dependent manner. The results provide supporting data that C. quadrangularis may contain active compounds useful in treating anti-inflammatory, anti-diabetic disorders.

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“…The molecular mass of the purified protease was estimated 19.38 kDa. Most of the characterized cysteine proteases are small proteins with molecular masses in the range of 20 to 40 kDa 2, [32][33][34][35][36][37][38][39][40][41][42] .…”

Section: Discussionmentioning

confidence: 99%

Cohnella 1759 cysteine protease shows significant long term half-life and impressive increased activity in presence of some chemical reagents

Saghian

Mokhtari

Aminzadeh

2021

Sci Rep

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Thermostability and substrate specificity of proteases are major factors in their industrial applications. rEla is a novel recombinant cysteine protease obtained from a thermophilic bacterium, Cohnella sp.A01 (PTCC No: 1921). Herein, we were interested in recombinant production and characterization of the enzyme and finding the novel features in comparison with other well-studied cysteine proteases. The bioinformatics analysis showed that rEla is allosteric cysteine protease from DJ-1/ThiJ/PfpI superfamily. The enzyme was heterologously expressed and characterized and the recombinant enzyme molecular mass was 19.38 kD which seems to be smaller than most of the cysteine proteases. rEla exhibited acceptable activity in broad pH and temperature ranges. The optimum activity was observed at 50℃ and pH 8 and the enzyme showed remarkable stability by keeping 50% of residual activity after 100 days storage at room temperature. The enzyme Km and Vmax values were 21.93 mM, 8 U/ml, respectively. To the best of our knowledge, in comparison with the other characterized cysteine proteases, rEla is the only reported cysteine protease with collagen specificity. The enzymes activity increases up to 1.4 times in the presence of calcium ion (2 mM) suggesting it as the enzyme’s co-factor. When exposed to surfactants including Tween20, Tween80, Triton X-100 and SDS (1% and 4% v/v) the enzyme activity surprisingly increased up to 5 times.

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Antibacterial cysteine protease from Cissus quadrangularis L.

Cited by 14 publications

References 47 publications

Ethnoveterinary Study of Plant‐Based Remedies for Treating Diseases in Small Ruminants in Maputo Province, Mozambique

Ethnoveterinary Study of Plant‐Based Remedies for Treating Diseases in Small Ruminants in Maputo Province, Mozambique

In vitro anti-inflammatory, anti-diabetic and antioxidant potential of Cissus quadrangularis along with its orexigenic activity in Drosophila melanogaster

Cohnella 1759 cysteine protease shows significant long term half-life and impressive increased activity in presence of some chemical reagents

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