Investigation of Photoacoustic imaging for monitoring of Wound Healing under a Layer of Blood with Different Coagulation

Song, Chul-Gyu; Kim, Keo-Sik; Kim, Minho; Ryu, Sang-Hun

doi:10.1109/icawst.2011.6163166

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…PAI has shown great potential for biomedical imaging applications including wound and burn healing monitoring, 22 , 23 functional brain imaging, 24 and cancer detection and therapy monitoring. 6 , 25 While these results are promising, depth restrictions have limited these studies to mostly superficial, accessible tissues and small animal pre-clinical models.…”

Section: Discussionmentioning

confidence: 99%

Performance Characteristics of Photoacoustic Imaging Probes with Varying Frequencies and Light-delivery Schemes

et al. 2019

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Photoacoustic imaging (PAI) is an emerging biomedical imaging technique that utilizes a combination of light and ultrasound to detect photoabsorbers embedded within tissues. While the clinical utility of PAI has been widely explored for several applications, limitations in light penetration and detector sensitivity have restricted these studies to mostly superficial sites. Given the importance of PA signal generation and detection on light delivery and ultrasound detector frequency, there is an ongoing effort to optimize these parameters to enhance photoabsorber detection at increased depths. With this in mind, in this study we examined performance benchmarks of a commercially available PAI/ultrasound linear array system when using different imaging frequencies and light delivery schemes. A modified light fiber jacket providing focused light delivery (FLD) at the center of the probe was compared with the built-in fiber optics lining the length of the probe. Studies were performed in vitro to compare performance characteristics such as imaging resolution, maximum imaging depth, and sensitivity to varying hematocrit concentration for each frequency and light delivery method. Monte Carlo simulations of each light delivery method revealed increased light penetration with FLD. In tissue-mimicking phantoms, vascular channels used to simulate blood vessels could be visualized at a depth of 2.4 cm when lowering imaging frequency and utilizing FLD. Imaging at lower frequencies with FLD also enabled enhanced detection of varying hematocrit concentration levels at increased depths, although lateral imaging resolution was reduced. Finally, a proof of concept in vivo probe comparison study in a mouse tumor model provided supportive evidence of our in vitro results. Collectively, our findings show that adjusting imaging frequency and applying FLD can be a straightforward approach for improving PAI performance.

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Section: Discussionmentioning

confidence: 99%

Performance Characteristics of Photoacoustic Imaging Probes with Varying Frequencies and Light-delivery Schemes

et al. 2019

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“…Future work will also include the 3D capability of commercial systems like the Vevo LAZR-X to obtain 3D PA and PE volume data with the HWG retaining contact with the tissue sample or animal. Reflection-mode PA systems with increased capabilities also have promise in clinical environments to aid in the diagnosis of superficial skin lesions relating to skin cancer or tracking the progression of wound healing 7 , 53 , 54 . Efforts are underway to incorporate HWG into a novel closed-loop reflection-mode PA system tailored for imaging skin lesions and monitoring wound healing.…”

Section: Discussionmentioning

confidence: 99%

“…Reflection-mode PA systems with increased capabilities also have promise in clinical environments to aid in the diagnosis of superficial skin lesions relating to skin cancer or tracking the progression of wound healing. 7,53,54 Efforts are underway to incorporate HWG into a novel closed-loop reflection-mode PA system tailored for imaging skin lesions and monitoring wound healing. A patent application has been issued for the HWG interface mechanism as described in this article.…”

Section: Discussionmentioning

confidence: 99%

Heavy water coupling gel for short-wave infrared photoacoustic imaging

Salinas,

Reichel,

Gupta

et al. 2023

J. Biomed. Opt.

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Significance: Changes in lipid, water, and collagen (LWC) content in tissue are associated with numerous medical abnormalities (cancer, atherosclerosis, and Alzheimer's disease). Standard imaging modalities are limited in resolution, specificity, and/or penetration for quantifying these changes. Short-wave infrared (SWIR) photoacoustic imaging (PAI) has the potential to overcome these challenges by exploiting the unique optical absorption properties of LWC > 1000 nm.Aim: This study's aim is to harness SWIR PAI for mapping LWC changes in tissue. The focus lies in devising a reflection-mode PAI technique that surmounts current limitations related to SWIR light delivery.Approach: To enhance light delivery for reflection-mode SWIR PAI, we designed a deuterium oxide (D 2 O, "heavy water") gelatin (HWG) interface for opto-acoustic coupling, intended to significantly improve light transmission above 1200 nm.Results: HWG permits light delivery >1 mJ up to 1850 nm, which was not possible with water-based coupling (>1 mJ light delivery up to 1350 nm). PAI using the HWG interface and the Visualsonics Vevo LAZR-X reveals a signal increase up to 24 dB at 1720 nm in lipid-rich regions.Conclusions: By overcoming barriers related to light penetration, the HWG coupling interface enables accurate quantification/monitoring of biomarkers like LWC using reflection-mode PAI. This technological stride offers potential for tracking changes in chronic diseases (in vivo) and evaluating their responses to therapeutic interventions.

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“…In this work, we present the development of a chitosan/graphene oxide (Chi-GO)based hemostatic composite and multi-scale photoacoustic evaluation of its hemostatic performance, as shown in Figure 1. Photoacoustic (PA) imaging can provide optical contrast, deep penetration, high spatial resolution, and molecular information [34][35][36][37][38][39][40], and it has been used to monitor wound healing under a blood layer with a different degree of coagulation [41]. Hence, the hemostatic material and the wound-healing process can be visualized in vivo.…”

Section: Introductionmentioning

confidence: 99%

Multi-Scale Photoacoustic Assessment of Wound Healing Using Chitosan–Graphene Oxide Hemostatic Sponge

2021

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Hemostasis is vital to save lives, reducing risks of organ failure and hemorrhagic shock. Exploring novel hemostatic materials and precise monitoring of the hemostatic status is of great importance for efficient hemostasis. We present the development of chitosan–graphene oxide-based hemostatic composite and multi-scale photoacoustic evaluation of the hemostatic performance. The hemostatic sponge can quickly and efficiently absorb the blood with its porous cavity and specific surficial property. We inspect the hemostatic performance via an in vitro blood absorption test and in vivo mouse bleeding injury experiments. Results show that the synthesized hemostatic sponge can not only absorb plasma in blood fast with its interior porous structure but also stimulate the interfacial reaction with erythrocytes and platelets. The superiority of multi-scale photoacoustic imaging for guiding, monitoring, and evaluating the hemostatic stages of sponges is demonstrated with high spatial resolution and great sensitivity at depths. Photoacoustic evaluation of a chitosan–graphene oxide-based hemostatic sponge has the potential to be transferred toward the clinical assessment of wound healing.

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Investigation of Photoacoustic imaging for monitoring of Wound Healing under a Layer of Blood with Different Coagulation

Cited by 3 publications

References 7 publications

Performance Characteristics of Photoacoustic Imaging Probes with Varying Frequencies and Light-delivery Schemes

Performance Characteristics of Photoacoustic Imaging Probes with Varying Frequencies and Light-delivery Schemes

Heavy water coupling gel for short-wave infrared photoacoustic imaging

Multi-Scale Photoacoustic Assessment of Wound Healing Using Chitosan–Graphene Oxide Hemostatic Sponge

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