Investigation of Oxidative Stress and Function of Mitochondria in Mononuclear Leukocytes of Blood in Patients With Chronic Bronchitis and With Chronic Obstructive Pulmonary Disease

Belskikh, Eduard; Uryasev, Oleg; Zvyagina, V I; Faletrova, Svetlana V.

doi:10.23888/hmj201862203-210

Cited by 5 publications

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References 5 publications

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“…Other data indicate that the inflammatory process significantly reduces the activity of key enzymes of anaerobic oxidation and ATP reserves in neutrophils, leading to the formation of secondary mitochondrial dysfunction [27]. We confirmed that in pregnant women with an average volume of lung changes, cytochemical indicators of succinate dehydrogenase significantly decreased, which could indicate a breakdown in the mechanisms of rapid adaptation in response to hypoxia and the accumulation of active forms of oxygen [28]. The same changes were found in the indicators of myeloperoxidase and lysosomal cationic proteins.…”

Section: Discussionsupporting

confidence: 80%

Disruption of the Functional Activity of Neutrophil Granulocytes as a Risk Factor for the Development of Lung Damage in Pregnant Women with COVID-19

Andrievskaya,

Ustinov,

Lyazgian

et al. 2024

CIMB

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Currently, the assessment of immune status in patients with COVID-19 is limited to determining the count of polymorphonuclear leukocytes and the phagocytic function of neutrophils, which is insufficient to understand the regulatory role of innate immunity cells in the development of pneumonia. However, no such studies have been conducted in pregnant women with COVID-19. The aim of this study was to investigate the functional state of neutrophil granulocytes in order to identify predictors of pneumonia severity risk in pregnant women with COVID-19. A clinical characterization of pregnant women with COVID-19 in addition to minimal and average lung changes was provided. The composition and ratio of morphological forms of leukocyte cells were studied. Cytochemical studies of neutrophil granulocytes were carried out and calculations of the mean cytological index (MCI) for succinate dehydrogenase, myeloperoxidase, and cationic proteins were performed. The number of NETs in blood smears was counted. Independent predictors of pneumonia severity in pregnant women with COVID-19 were calculated using regression analysis. The quality of the model was assessed using ROC analysis. In pregnant women with COVID-19 and an average volume of lung changes, the number of polymorphonuclear leukocytes (p = 0.03) and band neutrophils (p = 0.002) in the blood was significantly higher than in pregnant women with minimal lung changes. The MCI indicators of succinate dehydrogenase, cationic proteins, and myeloperoxidase in pregnant women with COVID-19 were reduced in relation to the control group (p < 0.0001). In blood smears of pregnant women with COVID-19 and an average volume of lung changes, the number of NETs increased (p = 0.002). Regression analysis showed that succinate dehydrogenase and NETs are independent predictors of pneumonia severity in pregnant women with COVID-19. Our study confirms the prognostic significance of low levels of neutrophilic succinate dehydrogenase and high levels of NETs in the blood of pregnant women with COVID-19. The combination of these two biomarkers is a significant reflection of the severity of pneumonia development in pregnant women with COVID-19. However, further research is needed to identify the mechanisms underlying this association.

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Section: Discussionsupporting

confidence: 80%

Disruption of the Functional Activity of Neutrophil Granulocytes as a Risk Factor for the Development of Lung Damage in Pregnant Women with COVID-19

Andrievskaya,

Ustinov,

Lyazgian

et al. 2024

CIMB

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“…Активное изучение роли вторичной митохондриальной дисфункции при ХОБЛ позволило установить её системный характер, что позволяет фиксировать нарушения функций митохондрий не только в клетках тканей легких, но и других органов, например в мышцах, клетках крови [5][6][7][8]. Поэтому большой интерес представляет изучение показателей адаптации к гипоксии митохондрий клеток периферической крови, которые наиболее доступны для исследования в рутинной клинической практике [9][10][11].…”

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“…В текущее пилотное исследование было включено 58 пациентов с ХОБЛ и 13 условно здоровых добровольцев в возрасте от 40 до 75 лет. Минимальный размер выборки рассчитывали с учетом ранее проведенных исследований и использованием калькулятора Open Epi со статистическими допущениями альфа-ошибки 5 и 95% доверительным интервалом (ДИ), с учетом снижения концентрации сукцината лейкоцитов крови, по меньшей мере, на 25% для 98% пациентов с ХОБЛ [11]. В группу больных с ХОБЛ включались пациенты проходившие лечение в ГБУ РО ОКБ (г. Рязань) и обратившиеся к пульмонологу ГБУ РО поликлиники №6 (г. Рязань) по поводу обострения заболевания.…”

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Succinate and succinate dehydrogenase of mononuclear blood leukocytes as markers of adaptation of mitochondria to hypoxia in patients with exacerbation of chronic obstructive pulmonary disease

Belskikh

Uryasiev

Zvyagina

et al. 2020

I.P. Pavlov Russian Medical Biological Herald

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Aim. To study the concentration of succinate and the activity of succinate dehydrogenase (SDH) of mononuclear blood leukocytes as markers of rapid adaptation of mitochondria to hypoxia in patients with exacerbation of chronic obstructive pulmonary disease (COPD). Materials and Methods. The study involved 58 patients with COPD and 13 conventionally healthy volunteers of 40-75 years of age. In accordance with GOLD 2018 principles of complex assessment, the patients were divided to groups B (n=18), C (n=20), D (n=20) comparable in age, FEV1 and in pack-of-cigarettes/year index. Patients of D group were characterized by more pronounced hypoxemia. Activity of SDH and concentration of succinate were determined in mononuclear leukocytes isolated from blood. Results. Patients with exacerbation of COPD divided to groups on the basis of the frequency of exacerbations and evidence of symptoms, were characterized by different severity of disorders of mitochondrial functions of mononuclear leukocytes. Patients of C group had the highest succinate concentration (428 [357;545] nmol/106 cells in I ml of suspension) and SDH activity (64[56;73] nmol of succinate/min * 106 cells of 1 ml of suspension) in mononuclear leukocytes as compared to groups B (1.43-times reduction of succinate, p0.002; 1.88-times reduction of SDH, p=0.0015) and D (2.06-times reduction of succinate, p0.0001; 4.26-times reduction of SDH, p0.0001). Patients of D group demonstrated the most pronounced reduction of markers of adaptation to hypoxia. Conclusions. A small amount of symptoms in exacerbation of COPD is associated with the highest parameters of the mechanism of rapid adaptation of mitochondria of mononuclear leukocytes to hypoxia. Existence of evident symptoms and frequent exacerbations in patents is associated with a severe frustration of mechanisms of adaptation of mitochondria to hypoxia.

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Analysis of oxidative modification of rat epididymal mitochondrial proteins under normobaric chronic hypoxia and modulation of nitric oxide (II) synthesis

Marsyanova

Zvyagina²,

Petrov³

2022

Kazan Med J

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Background. The molecular mechanism of NO effects in adaptation to hypoxia is of interest as a potential point of application in the therapy of fertility disorders. Aim. To assess the degree of oxidative modification of epididymal mitochondrial proteins during hypoxia under conditions of experimentally modified NO synthesis. Material and methods. Sexually mature rats were divided into four groups of 8 individuals: (1) control (animals without hypoxia); (2) chronic normobaric hypoxia, the animals were kept in a sealed chamber with oxygen reduced to 10% once a day for 14 days; (3) animals with hypoxia were injected with an inhibitor of the synthesis of nitric oxide (II) L-nitroarginine methyl ester at a dose of 25 mg/kg intraperitoneally once a day for 7 days; (4) animals with hypoxia were injected with the substrate for NO synthesis L-arginine at a dose of 500 mg/kg intraperitoneally once a day for 10 days. In the mitochondrial fraction of the head and tail of the epididymis, the activity of superoxide dismutase, the amount of NO metabolites, and the degree of oxidative modification of proteins were evaluated. Statistical analysis was performed using the ShapiroWilk, MannWhitney, and Spearman tests; differences were considered significant at p 0.05. Results. Hypoxia led to an increase in the oxidative modification of proteins in the mitochondria of the head of the epididymis 319.12 [240.98; 363.63] c.u./mg protein relative to control 17.89 [15.31; 27.62] c.u./mg protein, p=0.0009. In the mitochondria of the tail of the epididymis, the oxidation of proteins under the studied conditions was less pronounced. The use of L-nitroarginine methyl ester, as well as L-arginine, led to a decrease in the level of oxidative modification of proteins in the head of the epididymis relative to the hypoxia model 39.89 [29.25; 43.17] and 37.25 [34.91; 40.96] c.u./mg of protein, respectively, p=0.0009. Conclusion. Mitochondrial proteins in the head of the epididymis are more susceptible to oxidative damage during hypoxia; a decrease in the level of NO metabolites under conditions of oxygen deficiency is associated with a decrease in the oxidative modification of mitochondrial proteins.

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Investigation of Oxidative Stress and Function of Mitochondria in Mononuclear Leukocytes of Blood in Patients With Chronic Bronchitis and With Chronic Obstructive Pulmonary Disease

Cited by 5 publications

References 5 publications

Disruption of the Functional Activity of Neutrophil Granulocytes as a Risk Factor for the Development of Lung Damage in Pregnant Women with COVID-19

Disruption of the Functional Activity of Neutrophil Granulocytes as a Risk Factor for the Development of Lung Damage in Pregnant Women with COVID-19

Succinate and succinate dehydrogenase of mononuclear blood leukocytes as markers of adaptation of mitochondria to hypoxia in patients with exacerbation of chronic obstructive pulmonary disease

Analysis of oxidative modification of rat epididymal mitochondrial proteins under normobaric chronic hypoxia and modulation of nitric oxide (II) synthesis

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