Investigation of novel oligoelectrolyte polymer carriers for their capacity of DNA delivery into plant cells

Finiuk, Nataliya; Buziashvili, Anastasiia; Burlaka, O. M.; Zaichenko, Alexander; Mitina, Nataliya; Miagkota, Olesia; Lobachevska, О.V.; Stoika, Rostyslav; Blume, Ya. B.; Yemets, А. І.

doi:10.1007/s11240-017-1259-7

Cited by 32 publications

(17 citation statements)

References 54 publications

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“…Due to smaller size and surface effect of starch nanoparticles, the DNA-nanoparticle complex (pEGAD plasmid DNA-PLL-StNP) was protected from DNase I cleavage (Jun et al, 2008). Similarly, dimethylaminoethyl metacrylate (DMAEM) based cationic polymers protect DNA against cleavage by DNAse I due to repulsion of Mg 2+ ions by amino groups and hindrance of accessibility of enzymes to DNA (Finiuk et al, 2017). Further, endosomal escape and cytosolic stability were reported by MSNP (Hussain et al, 2013) in which MSNP functionalized with TEG remained stable in endocytotic vesicles in cytoplasm and CNT (Burlaka et al, 2015).…”

Section: Merits Of Nanoparticles Meditaed Transformation Over Conventional Methodsmentioning

confidence: 99%

“…Hussain et al (2013) witnessed the uptake of MSN by wheat root during post germination in MS liquid medium solution and lupin roots in hydroponic system and did not cause any toxic effect and nanoparticles were found to be internalized near and within the cell wall of wheat root and xylem of lupin roots. Finiuk et al (2017) studied genotoxic and cytotoxic properties of polymer PDMAEM based nanoparticles in meristem cells of Allium cepa roots and protoplasts of tobacco (Nicotiana tabacum) based on miotic index and number of viable protoplasts. It was observed that cationic polymers TN 84/5 was least cytotoxic, even at extreme concentration of polymer (5×10 -2 ).…”

Section: Merits Of Nanoparticles Meditaed Transformation Over Conventional Methodsmentioning

confidence: 99%

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A Review of Nanotechnology as a Novel Method of Gene Transfer in Plants

Begum

Jayawardana

2021

J Agric Sciences

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Purpose: Nanotechnology has evolved as an effective tool in numerous fields including agriculture, medicine and engineering. Recently it's potential as an alternative genetic transformation method has been identified. However, a comprehensive understanding over nanoparticles and their behavior in living cells is important to realize the full potential of this technology in biotechnological applications. Therefore, we review the application potential of widely employed nanoparticles in plant transformation here. Literature/Background: Development of new crop varieties with desirable traits via biotechnological applications is a solution for challenges associated with climate change and higher population growth.In such aspects, transformation of plant cells which is known as the process of changing one's genome by integration exogenous DNA, is an absolute necessity and results far better and improved stable characteristics in original. Rigid and multi layered cell wall impedes penetration of exterior biomolecules and hence causes the transformation process complicated. Even though, numerous conventional methods have been established for plant transformation, lower transformation efficiency, tissue damage and random integration of transgenes warrants the need for novel approaches. In this context, novel techniques have been explored and as a result nanoparticles have been found effective in transformation of protoplasts as well as intact plant cells. Nanoparticles internalized either via endocytosis or direct penetration release transgenes from nanoparticle-DNA complexes and result in transient or stable expression. Nanoparticles ensure higher transformation efficiency, no transgenic silencing and protection of biogenic molecules from degradation by intracellular nucleases.

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Section: Merits Of Nanoparticles Meditaed Transformation Over Conventional Methodsmentioning

confidence: 99%

Section: Merits Of Nanoparticles Meditaed Transformation Over Conventional Methodsmentioning

confidence: 99%

A Review of Nanotechnology as a Novel Method of Gene Transfer in Plants

Begum

Jayawardana

2021

J Agric Sciences

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“…The dsRNA and dendrimer polyplexes were able to penetrate through the root wall into the plant cells, leading to a significant reduction in shoot apical meristem size and increased lateral roots. In another report, 111 a number of dimethylaminoethyl methacrylate (DMAEMA)-based cationic polymers (Figure 7a) were evaluated for plasmid DNA binding capacity, protection against nuclease degradation, and transfection efficiency to Ceratodon purpureus moss as well as Nicotiana tabacum protoplasts. However, in general, the exploration of functional polymers in this area is at a very preliminary stage and requires significantly more research focus.…”

Section: Other Applications: Inside Plants Andmentioning

confidence: 99%

Recent Trends in Advanced Polymer Materials in Agriculture Related Applications

Sikder

Pearce

Parkinson

et al. 2021

ACS Appl. Polym. Mater.

118

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Over the past few decades, advanced polymeric materials have gained popularity in the development of sustainable agricultural applications. Smart polymeric systems have extensively contributed to the agricultural industry by increasing the efficiency of pesticides, herbicides, and fertilizers by facilitating controlled release systems and, therefore, enabling lower doses to be used. Superabsorbent polymeric materials have been used as soil conditioners to control the impact of drought, whereas polycationic polymers have been utilized for plant bioengineering. These functions in the environment are complemented by applications within plants as part of the developing range of tools for genetically transforming plants in order to increase productivity and disease resistance. This Review will summarize and discuss the recent developments in the design and application of advanced polymeric systems for precision agriculture related applications. The design criteria of the polymers employed to date, such as polymer structure, as well as the properties of polymer nanoparticles including shape and size will be discussed, and the key findings in the related area will be highlighted. Finally, we will identify future directions for the exploration of functional polymers with the ultimate aim of advancing sustainable agriculture.

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“…Nanotechnology-based methods have been used to deliver biomolecules and chemicals into cells in both plant and mammalian cell systems (Chang et al, 2013;Wang et al, 2014;Mahakham et al, 2017;Fortuni et al, 2019); however, compared with the mammalian cell delivery process, NP-mediated plant biomolecule delivery has proven to be more challenging because of the presence of the natural barrier provided by the cell wall (Mao et al, 2019). It has been suggested that the use of NPs enables an efficient plant transformation because NPs protect the genetic cargo from cellular enzymatic degradation (e.g., nucleases; Finiuk et al, 2017;Joldersma and Liu, 2018 (Bates and Kostarelos, 2013;Karimi et al, 2015), but metallic NPs can only deliver DNA as genetic cargo (Zhao et al, 2017). In addition, silicon-based NPs can carry DNA and proteins, whereas polymeric NPs (e.g., PEG and polyethyleneimine) can transfer encapsulated RNA, DNA, and proteins into cells (Silva et al, 2010;Moon et al, 2011;Su et al, 2011;Hasanzadeh Kafshgari et al, 2015;Zhou et al, 2018).…”

Section: Advanced Plant Biomolecule Delivery Approaches Via the Application Of Nanobiotechnologymentioning

confidence: 99%

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

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Investigation of novel oligoelectrolyte polymer carriers for their capacity of DNA delivery into plant cells

Cited by 32 publications

References 54 publications

A Review of Nanotechnology as a Novel Method of Gene Transfer in Plants

A Review of Nanotechnology as a Novel Method of Gene Transfer in Plants

Recent Trends in Advanced Polymer Materials in Agriculture Related Applications

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

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