Investigation of novel mediators for a glucose biosensor based on metal picolinate complexes

Warren, Susan; McCormac, Timothy; Dempsey, Eithne

doi:10.1016/j.bioelechem.2004.07.005

Cited by 24 publications

(15 citation statements)

References 27 publications

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“…There are several methods by which biosensors can be constructed using a mediator. Commonly used mediators are organic dyes [5][6][7], ferrocene/ferrocene derivatives [8][9][10], metal complexes [11,12], and similar compounds. Mediators are efficient at promoting a good response from an enzyme reaction under low overpotential.…”

Section: Introductionmentioning

confidence: 99%

Amperometric determination of glucose, based on the direct electron transfer between glucose oxidase and tin oxide

Park

Kim

Seong³

et al. 2008

Biotechnol Bioproc E

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An amperometric glucose biosensor was designed for the detection of glucose in blood, urine, beverages, and fermentation systems. In typical glucose biosensors that employ enzymes, mediators are used for efficient electron transfer between the enzymes and the electrode. However, some of these mediators are known to be toxic to the enzymes and also must be immobilized on the surface of the electrode. We propose a mediator-free glucose biosensor that uses a glucose oxidase immobilized on a tin oxide electrode. Direct electron transfer is possible in this system because the tin oxide has redox properties similar to those of mediators. The method for immobilization of the glucose oxidase onto the tin oxide is also very simple. Tin oxide was prepared by the anodization and annealing of pure tin, and this provides a large surface area for the immobilization step because of its porosity. Glucose oxidase was immobilized onto the tin oxide using the membrane entrapment method. The proposed method provides a simple process for fabricating the enzyme electrode. Glucose oxidase immobilized onto the tin oxide, prepared in accordance with this method, has a relatively large current response when compared to those of other glucose biosensors. The sensitivity of the biosensor was 19.55 µA/mM, and a linear response was observed between 0~3 mM glucose. This biosensor demonstrated good reproducibility and stability. © KSBB hÉóïçêÇëW=ÄáçëÉåëçêI=ÇáêÉÅí=ÉäÉÅíêçå=íê~åëÑÉêI=ÖäìÅçëÉI=ÖäìÅçëÉ=çñáÇ~ëÉI=íáå=çñáÇÉ= = = = =

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Section: Introductionmentioning

confidence: 99%

Amperometric determination of glucose, based on the direct electron transfer between glucose oxidase and tin oxide

Park

Kim

Seong³

et al. 2008

Biotechnol Bioproc E

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“…Mediated electrocatalysis is a powerful tool for electron transfer measurements of redox-active biomolecules. Such biosensors exhibit fast response, amplify the analytical signal and largely diminish interference from other reduction species [94,95]. However, the rate at which the mediator exchanges electrons with an enzyme is a critical factor in designing biosensors.…”

Section: Glucose Nanobiosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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Section: Nn′)(dmso-s)2]mentioning

confidence: 99%

“…One of the popular and typical Ru(II)N5O type complexes that includes a pic ligand is [Ru(pic)(bpy)2] + (bpy = 2,2′-bipyridine), which has been synthesized. [3][4][5] The crystal structure and 1 H NMR spectrum of [Ru(pic)(bpy)2](PF6) have been repotrted. 3,4 In the crystal structure, the dihedral angles between two pyridine rings within each 2,2′-bipyridyl ligand (10.3(3) and 14.4(2) ), are significantly larger than that in [Ru(bpy)3](PF6)2 (2.2(1) ).…”

mentioning

confidence: 99%

“…Warren et al isolated the Cl salt by converting it from PF6 salt using the ion-exchange resin. 5 We directly isolated a Ru(II) complex as Cl salt, [Ru(pic)(bpy)2]Cl, from the reaction mixture, and determined the crystal structure, as shown in Fig. 1 To the homogeneous dark-purple hydrolysis solution, a solution of Hpic (1.1 mmol) in ethanol (20 mL) was added, and the mixture was refluxed for 3 h. While refluxing, the solution turned from dark-purple to wine-red.…”

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confidence: 99%

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Crystal Structure of (2-Picolinato)bis(2,2′-bipyridine)ruthenium(II) Chloride

Toyama

Nakayasu

Nagao

2017

X-ray Structure Analysis Online

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11The coordination chemistry of ruthenium(II) complexes with polypyridyl ligands has been an area of great interest, because of their redox properties and photochemistry. 1 We reported on the syntheses, crystal structures, and isomerization reactions of the ruthenium(II) complexes bearing a di-2-pyridyl ketone (dpk); trans(Cl),cis(S)-[RuCl2(dpk-κ 2 N,O)(dmso-S)2] and cis(Cl),cis(S)-[RuCl2(dpk-κ2 The crystal structure of the trans(Cl),cis(S)-isomer revealed that the oxygen of the ketone group on the dpk ligand was able to act as an effective donor atom to from a N,O-coordination mode, which was kinetically more favorable than the N,N mode, but was thermodynamically unstable. In view of the hemilabile coordination ability of the N,O bidentate ligand, the 2-picolinic acid (Hpic) has great interest for us. One of the popular and typical Ru(II)N5O type complexes that includes a pic ligand is [Ru(pic)(bpy)2] + (bpy = 2,2′-bipyridine), which has been synthesized. [3][4][5] The crystal structure and 1 H NMR spectrum of [Ru(pic)(bpy)2](PF6) have been repotrted. 3,4 In the crystal structure, the dihedral angles between two pyridine rings within each 2,2′-bipyridyl ligand (10.3(3) and 14.4(2) ), are significantly larger than that in [Ru(bpy)3](PF6)2 (2.2(1) ). 6Although a greater twisting of bpy ligands has not been noticed in the literature, 3 we take notice of the greater twisting and the hydrogen-bonding interactions between two oxygen atoms of a pic in a Ru(II) complex cation and 3,3′-protons of a bpy ligand in a neighboring complex cation; Fig. S1 in which the hydrogen-bonding diagram is drawn according to the CIF file of [Ru(pic)(bpy)2](PF6). That is to say, the four O···H-C interactions between two Ru(II) complex cations allow the cations to come close to each other so that the bpy ligands are twisted. Similar O···H-C interactions between two O atoms of a ClO4 -ion and 3,3′-protons of a bpy ligand in {(μ-H2L)[Ru(bpy)2]2}(ClO4)2 (H4L = 1,5-diamino-9,10-anthraquinone) have also been observed, 7 in which the dihedral angles of the bpy ligands (9.9 and 10.4 ) are similar to those in [Ru(pic)(bpy)2](PF6). We thus feel a great interest in the structure of [Ru(pic)(bpy)2] + with another counter anion. Warren et al. isolated the Cl salt by converting it from PF6 salt using the ion-exchange resin. 5 We directly isolated a Ru(II) complex as Cl salt, [Ru(pic)(bpy)2]Cl, from the reaction mixture, and determined the crystal structure, as shown in Fig. 1 To the homogeneous dark-purple hydrolysis solution, a solution of Hpic (1.1 mmol) in ethanol (20 mL) was added, and the mixture was refluxed for 3 h. While refluxing, the solution turned from dark-purple to wine-red. The resulting wine-red solution was evaporated to dryness under a vacuum, and the residue was dissolved in 2 mL of water. An aqueous solution of HCl (1 M, 2 mL) was added to precipitate a brown-red solid.The brown-red solid, [Ru(pic)(bpy)2]Cl was collected by filtration, washed with a small amount of cold water, and dried in vacuo (0.93 mmol, 96%). The 1 H NMR spectr...

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Investigation of novel mediators for a glucose biosensor based on metal picolinate complexes

Cited by 24 publications

References 27 publications

Amperometric determination of glucose, based on the direct electron transfer between glucose oxidase and tin oxide

Amperometric determination of glucose, based on the direct electron transfer between glucose oxidase and tin oxide

Electrospinning-Based Nanobiosensors

Crystal Structure of (2-Picolinato)bis(2,2′-bipyridine)ruthenium(II) Chloride

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