Investigation of normal and malignant laryngeal tissue by autofluorescence imaging technique

Palasz, Zbigniew; Grobelny, Andrzej; Pawlik, E.M.; Frączek, Marcin; Zalesska−Kręcicka, Maria; Klimczak, Aleksandra; Kręcicki, Tomasz

doi:10.1016/j.anl.2003.07.012

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…Studies comparing neoplastic tissues from the cervix [41], larynx [42] and GI tract [43] with the relative controls, have shown a decrease in AF emission in the pathologic samples, due to the alteration of the connective stroma, in particular of collagen and elastic fibres.…”

Section: Discussionmentioning

confidence: 99%

Colonic and gastric cancer metastatic lymph nodes: applications of autofluorescence-based techniques

et al. 2009

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Lymphadenectomy and extended lymphadenectomy are still controversial issues in surgical oncology. The detection methods for metastatic lymph nodes include lymphoscintigraphy and radiolabelled antibody, but immunohistochemical sentinel lymph node (SLN) identification is commonly used. The potential diagnostic use of cell and tissue autofluorescence (AF) is well known.Here we review our studies on the application of AF-based techniques for diagnosing metastatic lymph nodes. We had previously demonstrated that AF imaging allows discrimination between hyperplastic and primary neoplastic lymph nodes. Our more recent studies show that the combination of autofluorescence microspectroscopy and multispectral imaging autofluorescence microscopy can be applied to the diagnosis of secondary neoplastic lymph nodes in gastric and colorectal cancer. These techniques have been validated by histochemical and immunohistochemical methods. Studies are in progress to select the best dye tracer for SLN identification, to improve image quality and to develop a software for automatic analysis of tissue AF to help the decision-making process during surgery.

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Section: Discussionmentioning

confidence: 99%

Colonic and gastric cancer metastatic lymph nodes: applications of autofluorescence-based techniques

et al. 2009

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“…Kraft et al documented that the autofluorescent endoscopy has higher sensitivity, specificity and accuracy than white-light endoscopy in the detection of premalignant and malignant lesions of the larynx [69]. Winiarski et al showed in their study of forty patients that cancerous laryngeal mucosa showed lesser autofluorescent illumination [42], whereas Palasz et al and Zargi et al conducted a study in laryngeal cancer patients, and it showed a similar pattern of autofluorescent loss at the green spectra [70,71]. Additionally, Fostiropoulos et al showed in their study of 152 patients with laryngeal lesions that autofluorescent imaging has a sensitivity of 98% vs. 88% and an accuracy of 97% vs. 90% compared with white-light endoscopy [72].…”

Section: Advantages Of Autofluorescence In Upper Aerodigestive Tract ...mentioning

confidence: 99%

Autofluorescence Image-Guided Endoscopy in the Management of Upper Aerodigestive Tract Tumors

Lazim

Kandhro

Menegaldo³

et al. 2022

IJERPH

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At this juncture, autofluorescence and narrow-band imaging have resurfaced in the medicine arena in parallel with current technology advancement. The emergence of newly developed optical instrumentation in addition to the discovery of new fluorescence biomolecules have contributed to a refined management of diseases and tumors, especially in the management of upper aerodigestive tract tumors. The advancement in multispectral imaging and micro-endoscopy has also escalated the trends further in the setting of the management of this tumor, in order to gain not only the best treatment outcomes but also facilitate early tumor diagnosis. This includes the usage of autofluorescence endoscopy for screening, diagnosis and treatment of this tumor. This is crucial, as microtumoral deposit at the periphery of the gross tumor can be only assessed via an enhanced endoscopy and even more precisely with autofluorescence endoscopic techniques. Overall, with this new technique, optimum management can be achieved for these patients. Hence, the treatment outcomes can be improved and patients are able to attain better prognosis and survival.

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“…magnetic resonance imaging (MRI) [1], computed tomography (CT) [2], ultra sonography (US) [3], bioluminescence [4], fluorescence [5], positron emission tomography (PET) [6], multi-dimensional power-doppler imaging [7], etc …). Each of these imaging techniques shows distinct advantages and limitations to discriminate healthy from tumor tissue areas [8].…”

Section: Introductionmentioning

confidence: 99%

Advanced Vibrational Spectroscopic Imaging of Human Tissue in Life Science

Pallua¹,

Pezzei

Schaefer³

et al. 2012

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Vibrational spectroscopic imaging has become an essential tool for tissue analyses in life science and represents a modern analytical technique enabling the detection and characterization of molecular components of biological samples. It is based on the absorption of IR radiation by vibrational transitions in covalent bonds and enables global analysis of samples, with resolution close to the cellular level. Advantage of vibrational spectroscopic imaging is the acquisition of local molecular expression profiles, while maintaining the topographic integrity of the tissue by avoiding time-consuming extraction, purification and/or separation steps, respectively. With this nondestructive analytical method it is possible to get both qualitative and quantitative information of heterogeneous samples and unique chemimorphological information about the tissue status, which represents an important benefit for future analytical interpretation of pathological changes of a tissue.

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Investigation of normal and malignant laryngeal tissue by autofluorescence imaging technique

Cited by 11 publications

References 12 publications

Colonic and gastric cancer metastatic lymph nodes: applications of autofluorescence-based techniques

Colonic and gastric cancer metastatic lymph nodes: applications of autofluorescence-based techniques

Autofluorescence Image-Guided Endoscopy in the Management of Upper Aerodigestive Tract Tumors

Advanced Vibrational Spectroscopic Imaging of Human Tissue in Life Science

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