“…Clustering, also called unsupervised classification of FTIR microscopic data, can be performed such that spectra within the same cluster are as similar as possible and spectra in different clusters are as dissimilar as possible where different types of cells may be separated within biological tissue. There are many clustering techniques that have been applied for hyperspectral images, such as hierarchical clustering, KM clustering, and FCM clustering, which increase the information content of FTIR imaging data dramatically [18]. For detailed information about statistical classification, the interested reader is referred to the cited literature.…”