Investigation of multipotent postnatal stem cells from human maxillary sinus membrane

Abstract

Maxillary sinus membrane (MSM) elevation is a common surgical technique for increasing bone height in the posterior maxilla prior to dental implant placement. However, the biological nature of bone regeneration in MSM remains largely unidentified. In this study, MSM tissue was obtained from 16 individuals during orthognathic surgery and used to isolate MSM stem cells (MSMSCs) by single-colony selection and STRO-1 cell sorting. The cell characteristics in terms of colony-forming ability, cell surface antigens, multi-differentiation potential and in vivo implantation were all evaluated. It was found that MSMSCs were of mesenchymal origin and positive for mesenchymal stem cell (MSC) markers such as STRO-1, CD146, CD29 and CD44; furthermore, under defined culture conditions, MSMSCs were able to form mineral deposits and differentiate into adipocytes and chondrocytes. When transplanted into immunocompromised rodents, MSMSCs showed the capacity to generate bone-like tissue and, importantly, maintain their MSC characteristics after in vivo implantation. These findings provide cellular and molecular evidence that MSM contains stem cells that show functional potential in bone regeneration for dental implant.

miR-1827 inhibits osteogenic differentiation by targeting IGF1 in MSMSCs

“…Normal human MSM samples were obtained as previously reported6. MSMSCs were isolated and cultured as previously reported6.…”

“…MSMSCs were isolated and cultured as previously reported6. BMSSCs were obtained from ScienCell Research Laboratory (San Diego, CA, USA).…”

“…Osteoprogenitor cells harvested from the maxillary sinus membrane have osteogenic potential both in vitro and in vivo 345. We have previously demonstrated that human maxillary sinus membrane stem cells (MSMSCs) have osteogenic differentiation potential in vitro 6. In addition, MSMSCs participate in bone formation in nude mice and can retain their stem cell-like properties after long-term in vivo transplantation6.…”

“…We have previously demonstrated that human maxillary sinus membrane stem cells (MSMSCs) have osteogenic differentiation potential in vitro 6. In addition, MSMSCs participate in bone formation in nude mice and can retain their stem cell-like properties after long-term in vivo transplantation6. Nevertheless, the molecular mechanisms of bone formation remain poorly understood.…”

Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p

“…Clinical studies have shown that maxillary sinus augmentation can be reached by elevating the MSM with or without grafting, which suggested that maxillary sinus membrane possesses osteogenic activity. Further studies have isolated and characterized maxillary sinus membrane stem cells (MSMSCs) in maxillary sinus membrane, which displayed stem cell properties similar to bone marrow mesenchymal stem cells (BMMSCs) . Our previous study demonstrated that MSMSCs could express MSC markers such as STRO‐1, CD146, CD29.…”