miR-1827 inhibits osteogenic differentiation by targeting IGF1 in MSMSCs

Abstract

We recently reported that maxillary sinus membrane stem cells (MSMSCs) have osteogenic potential. However, the biological mechanisms of bone formation remain unclear. In this study, we investigated the role and mechanisms of microRNAs (miRNAs) in the osteogenic differentiation of MSMSCs. The expression of miRNAs was determined in differentiated MSMSCs by comprehensive miRNA microarray analysis and quantitative RT-PCR (qRT-PCR). We selected miR-1827 for functional follow-up studies to explore its significance in MSMSCs. Here, miR-1827 was found to be up-regulated during osteogenic differentiation of MSMSCs. Over expression of miR-1827 inhibited osteogenic differentiation of MSMSCs in vitro, whereas the repression of miR-1827 greatly promoted cell differentiation. Further experiments confirmed that insulin-like growth factor 1 (IGF1) is a direct target of miR-1827. miR-1827 inhibited osteogenic differentiation partially via IGF1, which in turn is a positive regulator of osteogenic differentiation. Moreover, miR-1827 suppressed ectopic bone formation and silencing of miR-1827 led to increased bone formation in vivo. In summary, this study is the first to demonstrate that miR-1827 can regulate osteogenic differentiation. The increase in miR-1827 expression observed during osteogenesis is likely a negative feedback mechanism, thus offering a potential therapeutic target to address inadequate bone volume for dental implantation through inhibiting miR-1827.

Serum microRNA expression profiling in patients with multiple system atrophy

“…Since IGF-1 signaling has been revealed to be regulated by several miRNAs (10,11), it may be hypothesized that miRNAs and IGF-1 contribute to the pathogenesis of MSA.…”

Lnc‐NTF3‐5 promotes osteogenic differentiation of maxillary sinus membrane stem cells via sponging miR‐93‐3p

“…After the indicated number of days of culture, cells were stained with Alizarin Red S (Sigma, St. Louis, Missouri) according to the instruction from the manufacturer and imaged using an inverted Zeiss AxioObserver Z1 microscope (Carl Zeiss Meditec, Inc, Dublin, California). Alizarin Red staining quantification was performed according to our previously published methods . One milliliter of 10% cetylpyridinium chloride (Sigma, St. Louis, Missouri) was added to each well for 45 min.…”

“…Then the four groups of constructs were transplanted subcutaneously. The mice were sacrificed, and the transplants were harvested after 8 wk for further studies as described in our studies . The transplants were fixed with 10% buffered formalin and decalcified with 10% ethylenediaminetetraacetic acid (pH 8.0) for 2 wk.…”

“…Alizarin Red staining quantification was performed according to our previously published methods. 9 One milliliter of 10% cetylpyridinium chloride (Sigma, St. Louis, Missouri) was added to each well for 45 min. The absorbance was read at 562 nm.…”

“…6 . Evidence further showed that MSMSCs can differentiate into osteoblasts both in vitro and in vivo, [7][8][9][10] which represented a useful cell source of MSCs for bone tissue engineering.…”