Investigation of molecular masses and aggregation of β-d-glucan from Poria cocos sclerotium by size-exclusion chromatography

Creation of regenerated cellulose microspheres with diameter ranging from micron to millimeter for chromatography applications

“…The chromatographic packing materials for biopolymer separation are mostly prepared from polysaccharides [23,28]. Preparative SEC columns packed with microporous regenerated cellulose gels have been used to fractionate biopolymers such as dextran [29],ˇ-dglucan from Poria cocos sclerotium [30] and enzyme [31].…”

Formation and structure of pachyman aggregates in dimethyl sulfoxide containing water

“…21 Previously, we have investigated the polysaccharide PC3, a linear ␤-(1 3 3)-D-glucan from the sclerotium of Poria cocos, and found the aggregation phenomenon in aqueous solution using dynamic laser light scattering, analytical size-exclusion chromatography (SEC), viscometry, and membrane osmometry under different conditions. [22][23][24] These results proved that the polysaccharide formed aggregates in aqueous solution or in dimethyl sulfoxide (DMSO) with LiCl absorbed moisture, and the aggregates can be broken by DMSO without water, cadoxen, or at 80°C. The purpose of this article is to study in detail the kinetics and geometry of polysaccharide aggregates by static and dynamic LLS to elucidate the feature of aggregates using different models.…”

Molecular weight and arm number of a star‐shaped styrene–butadiene block copolymer synthesized on a pilot‐vessel scale

“…We have accumulated experience in the fractionation and characterization of the molecular weights and chain conformations of polymers. [23][24][25][26][27] In this work, we attempted to prepare starshaped SBS copolymers by living anionic polymeriza-tion with divinylbenzene (DVB) as a linking agent on a pilot-vessel scale, and then we studied their molecular weights, arm numbers, and chain shapes. We hope that this work contributes toward a meaningful pathway that can help us develop the production of star-shaped SBS copolymers.…”