Formation and structure of pachyman aggregates in dimethyl sulfoxide containing water

Ding, Qiong; Zhang, Lina; Wu, Chi

doi:10.1002/(sici)1099-0488(19991115)37:22<3201::aid-polb4>3.0.co;2-5

Cited by 14 publications

(4 citation statements)

References 26 publications

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“…We measured the M w , 〈 s 2 〉 1/2 and [η] of the renatured lentinan, and results are shown in Figures 3 and 4 (solid circle and solid triangle), indicating that the M w , 〈 s 2 〉 1/2 , and [η] come back to the magnitudes of the triple‐helix lentinan. However, the linear β‐(1→3)‐ D ‐glucan from the Poria cocos mycelia exists as aggregate30, 31 in aqueous solution by dialyzing against water in the same condition, indicating that the restructuration of disrupted single β‐(1→3)‐ D glucan is very complicated and interesting. The mechanism of renaturation is not well known and still under further investigation.…”

Section: Resultsmentioning

confidence: 99%

Morphologies and conformation transition of lentinan in aqueous NaOH solution

Zhang

2004

Biopolymers

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Molecular morphologies and conformation transition of lentinan, a beta-(1-->3)-D-glucan from Lentinus edodes, were studied in aqueous NaOH solution by atomic force microscopy (AFM), viscometry, multiangle laser light scattering, and optical rotation measurements. The results revealed that lentinan exists as triple-helical chains and as single random-coil chains at NaOH concentration lower than 0.05M and higher than 0.08M, respectively. Moreover, the dramatic changes in weight-average molecular weight Mw, radius of gyration [s2](1/2), intrinsic viscosity [eta], as well as specific optical rotation at 589 nm [alpha]589 occurred in a narrow range of NaOH concentration between 0.05 and 0.08M NaOH, indicating that the helix-coil conformation transition of lentinan was carried out more easily than that of native schizophyllan and scleroglucan, and was irreversible. For the first time, we confirmed that the denatured lentinan molecule, which was dissolved in 0.15M NaOH to be disrupted into single coil chains, could be renatured as triple helical chain by dialyzing against abundant water in the regenerated cellulose tube at ambient temperature (15 degrees C). In view of the AFM image, lentinan in aqueous solution exhibited the linear, circular, and branched species of triple helix compared with native linear schizophyllan or scleroglucan.

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Section: Resultsmentioning

confidence: 99%

Morphologies and conformation transition of lentinan in aqueous NaOH solution

Zhang

2004

Biopolymers

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“…Both glycopolymers exhibited a significant decrease in hydrodynamic size in DMSO compared to TRIS buffer. DMSO is a strong polar solvent and hydrogen-bond acceptor and has been shown to disrupt intermolecular and intramolecular hydrogen bonds of natural polysaccharides. − PGalEAm (Figure , left) at 1 mg/mL displays one size distribution in both aqueous and organic solvents (≤50 nm), attributed to the unaggregated molecule. This is assumed based on the theoretical calculation for the radius of gyration ( R g ); for a polymer coil in a good solvent, R g is approximated by eq : where N is the degree of polymerization.…”

Section: Resultsmentioning

confidence: 99%

Effects of Stereochemistry and Hydrogen Bonding on Glycopolymer–Amyloid-β Interactions

et al. 2020

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“…The polysaccharides in aqueous solution exhibit different chain conformation such as single helix 19. double helix,20, 21 triple helix,22 random coil,23 and aggregate,24, 25 even if they are glucan.…”

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confidence: 99%

Chemical structure and chain conformation of the water-insoluble glucan isolated fromPleurotus tuber-regium

Zhang

Dong

et al. 2001

Biopolymers

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A water‐insoluble polysaccharide (TM8) was isolated from sclerotium of Pleurotus tuber‐regium by extraction with 0.5M NaOH aqueous solutions at 120°C. Its chemical structure was confirmed by infrared, high performance liquid chromatography, gas chromatography, and 13C NMR in dimethylsulfoxide (DMSO) to be composed of β‐(1 → 3)‐D‐glucan backbone chain linked with a branched glucose, one out of every three glycosyl units being substituted at C6 position. The glucan TM8 in DMSO was fractionated by nonsolvent addition method into ten fractions, and the solution properties were studied by size exclusion chromatography combined with multiangle laser light scattering (SEC‐MALLS) and viscometry in DMSO at 30°C. The dependencies of intrinsic viscosity [η] and radius of gyration 〈s2〉z1/2 on weight‐average molecular mass Mw for this glucan were found to be [η] = (9.24 ± 0.2) × 10−2Mw0.51 ± 0.02 (cm3g−1) and 〈s2〉z1/2 = (3.67 ± 0.3) × 10−2Mw0.56 ± 0.02 (nm) in the range of Mw from 1.07 × 104 to 77.4 × 104. Based on current theories for a wormlike chain, the conformational parameters of the glucan TM8 were found to be 408 (nm−1) for ML, 3.1 (nm) for q, and 16.8 for C∞, suggesting that the polysaccharide exists as a dense random‐coil chain in DMSO, due to branched structure. © 2001 John Wiley & Sons, Inc. Biopolymers 59: 457–464, 2001

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Formation and structure of pachyman aggregates in dimethyl sulfoxide containing water

Cited by 14 publications

References 26 publications

Morphologies and conformation transition of lentinan in aqueous NaOH solution

Morphologies and conformation transition of lentinan in aqueous NaOH solution

Effects of Stereochemistry and Hydrogen Bonding on Glycopolymer–Amyloid-β Interactions

Chemical structure and chain conformation of the water-insoluble glucan isolated fromPleurotus tuber-regium

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