Investigation of Microencapsulated BSH ActiveLactobacillusin the Simulated Human GI Tract

Abstract

This study investigated the use of microencapsulated bile salt hydrolase (BSH) overproducing Lactobacillus plantarum 80 cells for oral delivery applications using a dynamic computer-controlled model simulating the human gastrointestinal (GI) tract. Bile salt deconjugation rates for microencapsulated BSH overproducing cells were 4.87 ± 0.28 μmol/g microcapsule/h towards glycoconjugates and 0.79 ± 0.15 μmol/g microcapsule/h towards tauroconjugates in the simulated intestine, a significant (P < .05) increase over microencapsulated wild-type cells. Microcapsules protected the encased cells in the simulated stomach prior to intestinal release, maintaining cell viability above 10 9 cfu/mL at pH 2.5 and 3.0 and above 10 6 cfu/mL at pH 2.0 after 2-hour residence times. In the simulated intestine, encased cell viability was maintained above 10 10 cfu/mL after 3, 6, and 12-hour residence times in bile concentrations up to 1.0%. Results show that microencapsulation has potential in the oral delivery of live BSH active bacterial cells. However, in vivo testing is required.

Probiotic Bile Salt Hydrolase: Current Developments and Perspectives

“…Lactobacillus reuteri microcapsules also exhibited an improved deconjugation with 49.4±6.21% of glycoconjugates per hour in a simulated human gastrointestinal (GI) model and complete deconjugation after 4 h [56]. Bile salt deconjugation rates of microencapsulated BSH overproducing cells of L. plantarum 80 were 4.87±0.28 μmol/g microcapsule per hour towards glycoconjugates and 0.79± 0.15 μmol/g microcapsule per hour towards tauroconjugates in the simulated gastro-intestine [91].…”

Biotransformation of anthocyanins from two purple-fleshed sweet potato accessions in a dynamic gastrointestinal system

“…In order of sequence (reactors 1-5): the stomach (V1), small intestine (V2; duodenum, jejunum and ileum), the ascending (V3), the transverse (V4) and the descending colon (V5). The system is fully computer-controlled and has been validated using enumeration procedures, short chain fatty acid production patterns, enzymatic activities, gas production, and by microorganism-associated activities (Blanquet-Diot et al, 2009;Martoni et al, 2007;Molly et al, 1994).…”

“…The dynamic in vitro digestion system mimics the in vivo dynamics of transit during digestion and considers the varying microbial or digestive conditions in different segments of the GI tract including the ascending colon, transverse colon and descending colon. Adjustment of the pertinent pH for each colonic region leads to differences in growth of corresponding microbial communities that results in alterations in the metabolic activity of the colonic bioreactors (Blanquet-Diot et al, 2009;Martoni et al, 2007;Molly, Vande Woestyne, De Smet, & Verstraete, 1994).…”

Growth of Lactobacillus reuteri NCIMB 30242 during yogurt fermentation and bile salt hydrolysis activity in the product

“…Therefore, this test is technically a "bile salt hydrolysis" analysis from multiple bile salt hydrolase sources rather than a "bile salt hydrolase" test of a cell-free enzyme extract. The bile salt hydrolysis activity (BSHA) was carried out as described by Martoni et al (2007) with modifications. Activity tests were carried out on samples having approximately 10 8 CFU.mL…”

“…This was followed by filtration of the supernatant (0.45 μ) and analysis by HPLC. The HPLC analysis was carried out as described in Martoni et al (2007).…”