Investigation of Microbiological Methods to Estimating Individual Caries Risk: Evaluation of Sampling Methods and Materials.

Neta, Tomotake; Inokuchi, Ritsuko; Shinozaki-Kuwahara, Noriko; Kouno, Yoshiharu; Ikemi, Takuji; Fukushima, Kazuo

doi:10.5466/ijoms.1.29

Cited by 7 publications

(15 citation statements)

References 21 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, MS species are mainly found in plaque on the tooth surface [1]. Therefore, a bacterial count of plaque is required to clarify the caries-related activity of MS. A previous study that examined plaque collection methods to enable quantitation of bacteria revealed that plaque suspension after brushing teeth (brushing-plaque sample) is the best option [20]. In the present study, bacteriological test results were analyzed and evaluated using a culture method to quantitate S. sobrinus and S. mutans in brushing-plaque samples.…”

Section: Citationmentioning

confidence: 99%

“…Subjects with any systemic disease, using medications affecting salivary secretions, taking antibiotics were excluded from the study. The selected individuals were instructed not to eat/drink, use a mouth wash, or smoke 3 h prior to their appointment.Each subject was instructed to rinse the mouth, and then a large portion of plaque from all teeth was scraped off by vigorous brushing for 1 min using a sterile toothbrush, and was collected into a sterile bottle through a mouth rinse for 30s with 5ml phosphate-buffered saline and used as brushing-plaque sample [20].…”

Section: Subjects and Preparation Of Plaque Simplementioning

confidence: 99%

“…A stereomicroscope was used to differentiate between S. mutans and S. sobrinus on the basis of the morphology of colonies formed with sucrose, the differences arising from how glucan is synthesized by the GTF enzymes formed outside or on the outer layer of the bacterial body about ratio of cariogenic bacteria. The Enzyme Linked Immunosorbent Assay (ELISA) was also used in cases where differentiation proved difficult [20,21].…”

Section: Bacterial Analysismentioning

confidence: 99%

“…However, as it is difficult to quantify plaque samples, paraffin-or chewing gum-stimulated saliva is more commonly used for the assessment of dental caries risk [2,4,5]. Among such sampling methods, the processing of plaque samples collected by brushing (plaque suspension) allows for quantitation and simple adjustments [20]. Therefore, plaque may be a clinically useful sample.…”

Section: Introductionmentioning

confidence: 99%

“…Assessment of cariogenic bacteria levels is often used to determine the number of cariogenic bacteria [1][2][3][4][5]. However, few studies have reported the utility of the ratio of MS to total streptococci (TS) (MS/TS ratio) or S. mutans to TS (Sm/TS ratio) as an alternative approach to evaluate cariogenic bacteria [20,21]. Although the validity and reproducibility of these methods have been evaluated, an index must have an association with dental caries to be a useful standard to assess the risk of dental caries [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Evaluation of Dental Caries and Concomitant Infection Based on the Ratio of Cariogenic Bacteria using Plaque Samples in Adults

Gotouda¹,

Shinozaki-Kuwahara²,

Taguchi³

et al. 2017

Dentistry

View full text Add to dashboard Cite

show abstract

Section: Citationmentioning

confidence: 99%

Section: Subjects and Preparation Of Plaque Simplementioning

confidence: 99%

Section: Bacterial Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Dental Caries and Concomitant Infection Based on the Ratio of Cariogenic Bacteria using Plaque Samples in Adults

Gotouda¹,

Shinozaki-Kuwahara²,

Taguchi³

et al. 2017

Dentistry

View full text Add to dashboard Cite

show abstract

Bacteriological evaluation of mutans streptococci using modified mitis-salivarius-bacitracin (MSB) agar medium in primary dentition period

Saito

Ueda

Teramoto

et al. 2007

Pediatric Dental Journal

View full text Add to dashboard Cite

Mutans streptococci considered causative agents of dental caries are indigenous to the oral cavity. Modified mitis-salivarius-bacitracin (MSB) agar medium was supplied by BML. This media has characters to grow mutans streptococci very well and to inhibit of non-mutans streptococci. However, little is known about studies using this medium. In this study, we assessed the utility of modified MSB medium and discuss the relation between mutans streptococci and dental caries in primary dentition period. Modified MSB medium was found to be a suitable medium to isolate and quantify mutans streptococci since it could permit selective growth of mutans streptococci. Moreover, this medium inhibited to non-mutans streptococci (S. anginosus and S. intermedius), completely. The detection rate of Streptococcus mutans and Streptococcus sobrinus increased in proportion to the severity of dental caries in the nursery children. From these results, modified MSB agar medium is useful in the judgment to detect the mutans streptococci. mitis-salivarius-bacitracin (MSB) agar 9) (MS agar containing 200 U of bacitracin and 15% sucrose per liter) have been developed. However, non-mutans streptococci were found to form the colonies as well as mutans streptococci in these selective media 10) . Then modified MSB medium containing three kinds of antibiotics (2 mg of gramicidin D, 10 mg of colistin, and 10 mg of nalidixic acid per liter) in addition to bacitracin was supplied by BML (Tokyo, Japan) 11) . In this medium mutans streptococci can grow well, but only poor growth of non-mutans streptococci is permitted. Furthermore, S. mutans and S. sobrinus are distinguishable (by naked eyes) based on the different properties of colonies on modified MSB agar. The aim of this study is to assess the utility of this medium and discuss the relation between mutans streptococci and dental caries in primary dentition period.

show abstract

Microbial screening of Cariostat ® -inoculated plaque samples from low and high caries risk children

Ibrahim

Nishimura

Matsumura

et al. 2009

Pediatric Dental Journal

View full text Add to dashboard Cite

The aim of the present study was to investigate caries activity and it's association with the presence of S. mutans and S. sobrinus. The subjects were 499 children aged 2.5 years old, who participated in dental health examinations held from May 2006 to January 2007 at a health center in Katano City, Osaka, Japan. A dental examination was performed based on caries diagnostic criteria of the Health Policy Bureau, Ministry of Welfare, Japan. Plaque samples were collected using a sterile cotton swab from the maxillary buccocervical surfaces by the same dental hygienist. Two hundred seventy two (54.5%) children who had Cariostat score below 1.5 were determined as low risk and 227 (45.5%) who had a score above 2.0 as high risk. For microbial screening, 100 children were randomly selected from the high and low risk groups. The presence of S. mutans and S. sobrinus were detected using PCR technique. Thirty seven (18.5%) children had S. mutans alone, 20 (10.0%) had S. sobrinus alone, 16 (8.0%) had both S. mutans and S. sobrinus, and 127 (63.5%) had neither S. mutans nor S. sobrinus. Cariogenic bacteria were detected more from high caries risk than low caries risk children. A larger number of S. sobrinus was found in children with high df numbers than low df numbers. In this study, it was shown that S. sobrinus was more closely related with high caries risk activity and high df numbers. oral microorganisms that have virulence factors related to cariogenicity 7,8). These bacteria are acidogenic, aciduric and produce specific intra-and extrapolysaccharides that facilitate microbial adherence on teeth 8,9-12). Their amount and virulence, together with the patient's age at which these bacteria were contracted, are important factors in determining the course of this disease 3,13-15). Therefore, the levels of Mutans streptococci are considered to be important indicators for assessment of caries risk or management of caries prevention 7,16). The genetic diversity of these cariogenic bacteria is still controversial 18) , some suggesting that S. mutans has been shown to be more prevalent 1,19,20) and others found that S. sobrinus is more closely related with high caries activity and seldom found without S. mutans 18,21,22). Furthermore, Kozai et al. 23) and Klien

show abstract

Investigation of Microbiological Methods to Estimating Individual Caries Risk: Evaluation of Sampling Methods and Materials.

Cited by 7 publications

References 21 publications

Evaluation of Dental Caries and Concomitant Infection Based on the Ratio of Cariogenic Bacteria using Plaque Samples in Adults

Evaluation of Dental Caries and Concomitant Infection Based on the Ratio of Cariogenic Bacteria using Plaque Samples in Adults

Bacteriological evaluation of mutans streptococci using modified mitis-salivarius-bacitracin (MSB) agar medium in primary dentition period

Microbial screening of Cariostat ® -inoculated plaque samples from low and high caries risk children

Contact Info

Product

Resources

About