Investigation of Microalgal Carotenoid Content Using Coherent Anti‐Stokes Raman Scattering (CARS) Microscopy and Spontaneous Raman Spectroscopy

Legesse, Fisseha Bekele; Rüger, Jan; Meyer, Tobias; Krafft, Christoph; Schmitt, Michael; Popp, Jürgen

doi:10.1002/cphc.201701298

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…Previous reviews provide a good overview of applications and developments in CARS within a biological context (33)(34)(35). CARS images are constructed from spectral signatures of a particular molecule or macromolecule of interest such as pigments (36,37), fats (38,39), or water (40). The collected spectra can be used directly to study the specific molecular species or indirectly to study cellular-or tissue-level processes.…”

Section: Raman-based Methodsmentioning

confidence: 99%

“…The collected spectra can be used directly to study the specific molecular species or indirectly to study cellular-or tissue-level processes. Recent direct studies have investigated, for example, fat storage in Caenorhabditis elegans (41) and pigmentation in microalgae (36). Indirect studies have been important for understanding cancer and tumor proliferation (Figure 2) (37)(38)(39).…”

Section: Raman-based Methodsmentioning

confidence: 99%

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Microscopy and Cell Biology: New Methods and New Questions

Morris

Payne

2019

Annu. Rev. Phys. Chem.

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Understanding the cellular basis of human health and disease requires the spatial resolution of microscopy and the molecular-level details provided by spectroscopy. This review highlights imaging methods at the intersection of microscopy and spectroscopy with applications in cell biology. Imaging methods are divided into three broad categories: fluorescence microscopy, label-free approaches, and imaging tools that can be applied to multiple imaging modalities. Just as these imaging methods allow researchers to address new biological questions, progress in biological sciences will drive the development of new imaging methods. We highlight four topics in cell biology that illustrate the need for new imaging tools: nanoparticle-cell interactions, intracellular redox chemistry, neuroscience, and the increasing use of spheroids and organoids. Overall, our goal is to provide a brief overview of individual imaging methods and highlight recent advances in the use of microscopy for cell biology.

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Section: Raman-based Methodsmentioning

confidence: 99%

Section: Raman-based Methodsmentioning

confidence: 99%

Microscopy and Cell Biology: New Methods and New Questions

Morris

Payne

2019

Annu. Rev. Phys. Chem.

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“…Nowadays, the analysis of carotenoids in algae is performed using high-performance liquid chromatography (HPLC). However, it is important to emphasise that RS can be used for a more detailed analysis of carotenoids in algal cells [17,20,24]. In addition, the development of RS, which will be applied to algae in the future, can be used for real-time research of algal combination in nature.…”

Section: Application Of Raman Spectroscopy On Pigment Investigation I...mentioning

confidence: 99%

Raman Spectroscopy and Its Modifications Applied to Biological and Medical Research

Allakhverdiev

Khabatova

Kossalbayev

et al. 2022

Cells

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Nowadays, there is an interest in biomedical and nanobiotechnological studies, such as studies on carotenoids as antioxidants and studies on molecular markers for cardiovascular, endocrine, and oncological diseases. Moreover, interest in industrial production of microalgal biomass for biofuels and bioproducts has stimulated studies on microalgal physiology and mechanisms of synthesis and accumulation of valuable biomolecules in algal cells. Biomolecules such as neutral lipids and carotenoids are being actively explored by the biotechnology community. Raman spectroscopy (RS) has become an important tool for researchers to understand biological processes at the cellular level in medicine and biotechnology. This review provides a brief analysis of existing studies on the application of RS for investigation of biological, medical, analytical, photosynthetic, and algal research, particularly to understand how the technique can be used for lipids, carotenoids, and cellular research. First, the review article shows the main applications of the modified Raman spectroscopy in medicine and biotechnology. Research works in the field of medicine and biotechnology are analysed in terms of showing the common connections of some studies as caretenoids and lipids. Second, this article summarises some of the recent advances in Raman microspectroscopy applications in areas related to microalgal detection. Strategies based on Raman spectroscopy provide potential for biochemical-composition analysis and imaging of living microalgal cells, in situ and in vivo. Finally, current approaches used in the papers presented show the advantages, perspectives, and other essential specifics of the method applied to plants and other species/objects.

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“…The change in carotenoid content in diatoms under different light cycles was analyzed by tuning the CARS emission to 1528 cm −1 and Chl distribution was visualized by TPEF at 850 nm. 11 Recently, a HTS-Raman spectroscopy (HTS-RS) platform was developed to identify more than 100 000 single eukaryotic cells. 12 The current work adapted this platform to detect drug-and light-induced changes in pigment composition of individual living Phaedactylum tricornutum cells.…”

Section: Introductionmentioning

confidence: 99%