High-throughput screening Raman microspectroscopy for assessment of drug-induced changes in diatom cells

Abstract

16 888 Raman spectra were acquired with automated localization algorithms to investigate the effect of dithiothreitol on diatoms.

Application of High-Throughput Screening Raman Spectroscopy (HTS-RS) for Label-Free Identification and Molecular Characterization of Pollen

“…The measurements of pollen samples were performed on CaF 2 slides similar to the previous work [33,34] while the key differences are mentioned in the next paragraph. When samples were placed under the OBJ, a bright-field image also termed as field of view (FoV), was acquired.…”

“…In this work several system modifications were implemented in comparison to previously reported work [33,34], such as the development of a new image-based localization algorithm for pollen, the improvement of the autofocus function incorporating more points, as well as applying Gaussian curve fitting, the implementation of a homogenous illumination scheme for the bright field image. The improved autofocus function enables locating the best focal plane more precisely than the previous developments in [33,34] ensuring the Raman spectral quality whereas the homogenous illumination significantly improves the particle detection precision.…”

Automated Raman Micro-Spectroscopy of Epithelial Cell Nuclei for High-Throughput Classification

“…This paper builds on recent work in the development of robust Raman cytology platforms that are fully automated and high-speed. Schie et al have published several papers that describe a high-throughput automated Raman cytology platform with several applications [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. The basic platform [ 15 , 16 ] has several important features, including a defocused laser spot to excite a large cell area with high power, thereby facilitating shorter exposure times, and a LabVIEW-based automaton process, which identifies cell position using image processing and controls a translation stage to align the cells with the laser spot.…”

“…Drug testing is a key application area for automated Raman cytology systems. In this capacity, the same system was applied by the authors to (i) study the effect of dithiothreitol on the diatom Phaeodactylum tricornutum [ 17 ]; (ii) to analyse multi-well plates [ 18 ] for the purpose of developing a Raman-based cell viability assay; specifically, on the effect of doxorubicin concentration on monocytic THP-1 cells; and (iii) to investigate the effect of the targeted cancer drug panitumumab on colorectal cancer cell lines [ 20 ].…”

Automated Raman micro-spectroscopy of epithelial cells for the high-throughput classification

“…Drug testing is a key application area for automated Raman cytology systems. In this capacity, the same system has been applied by the authors to (i) study the effect of dithiothreitol on the diatom Phaedactylum tricornutum; [17] (ii) to analyse multi-well plates [18] for the purpose of developing a Raman-based cell viability assay; specifically, on the effect of doxorubicin concentration on monocytic THP-1 cells; and (iii) to investigate the effect of the targeted cancer drug panitumumab on colorectal cancer cell lines. [20]…”