Investigation of four 99mTc-labeled bacteriophages for infection-specific imaging

Rusckowski, Mary; Gupta, Suresh; Liu, Guozheng; Dou, Shuping; Hnatowich, Donald J.

doi:10.1016/j.nucmedbio.2008.02.011

Cited by 49 publications

(52 citation statements)

References 10 publications

(6 reference statements)

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“…The binding mechanism consists of the attachment of the phages to specific surface receptors or domains located on the surface of the bacterium and, subsequently, by transferring their genetic material into the host cell dedicated to phage replication/reproduction [113].…”

Section: Investigational/experimental Tracersmentioning

confidence: 99%

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Prosthetic joint infections: radionuclide state-of-the-art imaging

Gemmel¹,

Wyngaert²,

Love

et al. 2012

Eur J Nucl Med Mol Imaging

165

151

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Prosthetic joint replacement surgery is performed with increasing frequency. Overall the incidence of prosthetic joint infection (PJI) and subsequently prosthesis revision failure is estimated to be between 1 and 3%. Differentiating infection from aseptic mechanical loosening, which is the most common cause of prosthetic failure, is especially important because of different types of therapeutic management. Despite a thorough patient history, physical examination, multiple diagnostic tests and complex algorithms, differentiating PJI from aseptic loosening remains challenging. Among imaging modalities, radiographs are neither sensitive nor specific and cross-sectional imaging techniques, such as computed tomography and magnetic resonance imaging, are limited by hardware-induced artefacts. Radionuclide imaging reflects functional rather than anatomical changes and is not hampered by the presence of a metallic joint prosthesis. As a result scintigraphy is currently the modality of choice in the investigation of suspected PJI. Unfortunately, there is no true consensus about the gold standard technique since there are several drawbacks and limitations inherent to each modality. Bone scintigraphy (BS) is sensitive for identifying the failed joint replacement, but cannot differentiate between infection and aseptic loosening. Combined bone/gallium scintigraphy (BS/GS) offers modest improvement over BS alone for diagnosing PJI. However, due to a number of drawbacks, BS/GS has generally been superseded by other techniques but it still may have a role in neutropenic patients. Radiolabelled leucocyte scintigraphy remains the gold standard technique for diagnosing neutrophil-mediated processes. It seems to be that combined in vitro labelled leucocyte/bone marrow scintigraphy (LS/BMS), with an accuracy of about 90%, is currently the imaging modality of choice for diagnosing PJI. There are, however, significant limitations using in vitro labelled leucocytes and considerable effort has been devoted to developing alternative radiotracers, such as radiolabelled HIGs, liposomes, antigranulocyte antibodies and fragments, as well as more investigational tracers such as radiolabelled antibiotics, antimicrobial peptides, bacteriophages and thymidine kinase. On the other hand, positron emission tomography (PET) is still growing in the field of PJI imaging with radiotracers such as (18)F-fluorodeoxyglucose (FDG), (18)F-FDG white blood cells and (18)F-fluoride. But unfortunately this superb tomographic technique will only receive full acceptance when specific PET uptake patterns can be successfully developed. The emergence of hybrid modality imaging using integrated single photon emission computed tomography (SPECT) and PET with computed tomography (SPECT/CT and PET/CT) may also have a contributing role for more accurate assessment of joint replacement complications, especially combined with new radiotracers such as (68)Ga and (64)Cu. Finally, in searching for infection-specific tracers, currently there is no such diagnostic agent available.

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Section: Investigational/experimental Tracersmentioning

confidence: 99%

“…Unfortunately, only one radiolabelled bacteriophage showed any specificity for its host bacteria, indicating this approach needs major evaluation before further studies can be considered [114].…”

Section: Investigational/experimental Tracersmentioning

confidence: 99%

Prosthetic joint infections: radionuclide state-of-the-art imaging

Gemmel¹,

Wyngaert²,

Love

et al. 2012

Eur J Nucl Med Mol Imaging

165

151

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“…Bacteriophages are viruses that show no specificity for mammalian cells and infect bacteria exclusively (Rusckowski et al, 2004).…”

Section: Radiolabelled Bacteriophagesmentioning

confidence: 99%

Leukocyte and bacteria imaging in prosthetic joint infection

Glaudemans

Galli

Pacilio

et al. 2013

eCM

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There has been a significant increase in the number of joint prosthesis replacements worldwide. Although relatively uncommon, complications can occur with the most serious being an infection. Various radiological and nuclear imaging techniques are available to diagnose prosthetic joint infections (PJI). In this review article, we describe the pathophysiology of PJI, the principles of nuclear medicine imaging and the differences between Single Photon Emission Computed Tomography (SPECT) and Positron Emission Tomography (PET). The value of nuclear medicine techniques for clinical practice is also discussed.Then we provide an overview of the most often used radionuclide imaging techniques that may be helpful in diagnosing prosthetic joint infection: the 67 Ga-citrate, labelled white blood cells in vitro and in vivo (monoclonal antibodies directed against specific targets on the leukocytes), and 18 F-fluorodeoxyglucose ( 18 F-FDG). We describe their working methods, the pitfalls, and the interpretation criteria. Furthermore, we review recent advances in imaging bacteria, a molecular imaging method that holds promises for the detection of occult infections. We conclude proposing two diagnostic flow-charts, based on data in the literature, that could help the clinicians to choose the best nuclear imaging method when they have a patient with suspicion of or with proven PJI.

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“…M13 bakteriyofajı Tc-99m ile işaretlenmiş ve bakterilerde olduğu kadar fare modellerinde de hızlı tutulum gösterdiği saptanmıştır (62). Enfeksiyona özgül görüntüleme için potansiyel ajanlar geliştirmek üzere çeşitli radyoaktif bakteriyofajlar ile araştırmalar yapılmış ve sadece bir tanesi konak bakteriye özgüllük göstermiştir (63). Doğal immün sistemde önemli rolleri olan antimikrobiyal peptidler, mikrobiyal ataklara karşı korunmayı sağlarlar.…”

Section: Bakterinini̇n-vivogörüntülenmesiunclassified

Patient and Personnel Radiation Exposure in Sentinel Lymph Node Practice

Eren¹,

Bekiş²

2017

Nts

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Investigation of four 99mTc-labeled bacteriophages for infection-specific imaging

Cited by 49 publications

References 10 publications

Prosthetic joint infections: radionuclide state-of-the-art imaging

Prosthetic joint infections: radionuclide state-of-the-art imaging

Leukocyte and bacteria imaging in prosthetic joint infection

Patient and Personnel Radiation Exposure in Sentinel Lymph Node Practice

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