Investigation of Flavonoid Aglycones in Propolis Collected by Two Different Varieties of Bees in the Same Region

Koo, Michel Hyun; Park, Yong Kun

doi:10.1271/bbb.61.367

Cited by 49 publications

(23 citation statements)

References 1 publication

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“…However, unlike sodium hypochlorite and 4% chlorhexidine gluconate solutions, it was unable to completely eliminate them from the acrylic surfaces. Several studies have reported the antifungal activity of propolis (26,27), and it has also been stated that its mechanism may be related to the presence of flavonoids, phenolic acids, and their esters (24). TakaisiKikuni and Schilcher claimed that propolis brought about inhibition of DNA replication and, indirectly, cell division (44).…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of disinfectants on the adherence of Candida albicans to denture base resins with different surface textures

Karakış¹,

Akay

Oncul³

et al. 2016

J Oral Sci

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Abstract:The aim of the present study was to compare the effects of some disinfectants, including ethanol extract of propolis (EEP), on the adhesion of Candida albicans to denture base resins. Seventy-two acrylic resin samples were prepared, half of which was polished and the other half was roughened. C. albicans strain ATCC 10231 was incubated on Sabouraud dextrose agar (SDA) at 37°C for 48 h. The adhesion period was completed by keeping the cells in this suspension for 90 min at 37°C. Specimens were then immersed in the following solutions: 1%, 2%, and 5% sodium hypochlorite; 4% chlorhexidine gluconate; and 10% EEP. Quantification of the antifungal activity of the chemical solutions was performed using the colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay test. One-way ANOVA and post-hoc Tukey tests were performed to evaluate the effectiveness of chemical agents. Polished and roughened surfaces were compared using independent sample t-test. The mean surface roughness value was 0.35 (±0.04) µm for the polished group and 1.2 (±0.2) µm for the roughened group. The contact angles of both surfaces showed statistically significant difference, and 10% EEP solution exhibited significantly less removal of adherent viable C. albicans cells in both groups. All forms of sodium hypochlorite solutions yielded higher efficiency than 4% chlorhexidine gluconate and EEP solutions (P < 0.05). (J Oral Sci 58, 431-437, 2016)

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Section: Discussionmentioning

confidence: 99%

Effectiveness of disinfectants on the adherence of Candida albicans to denture base resins with different surface textures

Karakış¹,

Akay

Oncul³

et al. 2016

J Oral Sci

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“…Deionized water was used as a control for both AAs. PRO was prepared from an ethanolic extract of propolis (EEP) obtained from crude samples of Apis mellifera bees in the south of Brazil, classified as type SNB-RS (12,14).…”

Section: Antifungal Agentsmentioning

confidence: 99%

Effects of nystatin, fluconazole and propolis on poly(methyl methacrylate) resin surface

et al. 2008

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The prevalence of candidosis in denture wearers is as well established as its treatment with antifungal agents (AAs). However, little research has been done regarding the effects of AAs on denture base surfaces. Therefore, the aim of this study was to evaluate the effects of fluconazole (FLU), nystatin (NYS) and propolis orabase gel (PRO) on poly (methyl-methacrylate) (PMMA) surfaces. Deionized water and orabase gel without any active component were used as control groups. Conventional heat-polymerized (Clássico) and microwave-polymerized (Onda Cryl) acrylic resins were used. After polymerization, the specimens were polished and had their surfaces evaluated for roughness, free energy and Knoop hardness. Subsequently, specimens were immersed in AAs and controls for 14 days at 35±2 ºC and all variables were measured again. Data were analyzed statistically by 2-way ANOVA followed by Tukey's test ( α=0.05). Roughness results showed similar behavior for both PMMA resins, with PRO reaching the highest values and differing significantly from the other AAs (p<0.05). No statistically significant differences (p>0.05) were found between the two PMMA resins or between NYS and FLU as regards surface free energy. In conclusion, PRO was able to induce changes in PMMA surface properties, such as roughness, which could be related to microbial adhesion.

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“…Samples were chosen based on their effects on the growth of S. mutans and on a crude preparation of Gtf ]. The samples of propolis were dehydrated using a low-vacuum pump, and the EEP (10% w/v, in 80% aqueous ethanol, v/v) was prepared as described by Koo and Park [1997]. The extraction of propolis samples yielded 60 mg of resinous material/ml of EEP (ethanol-soluble fraction of propolis).…”

Section: Propolis Samplesmentioning

confidence: 99%

Effects of Apis mellifera Propolis on the Activities of Streptococcal Glucosyltransferases in Solution and Adsorbed onto Saliva–Coated Hydroxyapatite

et al. 2000

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Propolis, a resinous hive product collected by Apis mellifera bees, has been used for thousands of years in folk medicine. Ethanolic extracts of propolis (EEP) have been shown to inhibit the activity of a mixture of crude glucosyltransferase (Gtf) enzymes in solution. These enzymes synthesize glucans from sucrose, which are important for the formation of pathogenic dental plaque. In the present study, the effects of propolis from two different regions of Brazil on the activity of separate, purified Gtf enzymes in solution and on the surface of saliva–coated hydroxyapatite (sHA) beads were evaluated. The EEP from Minas Gerais (MG; Southeastern Brazil) and Rio Grande do Sul (RS; Southern Brazil) were tested for their ability to inhibit the enzymes GtfB (synthesis of insoluble glucan), GtfC (insoluble/soluble glucan) and GtfD (soluble glucan). The effects of propolis on Gtf from Streptococcus sanguis (soluble glucan synthesis) was also explored. The EEP from both regions effectively inhibited the activity of all Gtfs in solution (75–95%) and on the surface of sHA beads (45–95%) at concentrations between 0.75 and 3.0 mg of propolis/ml. However, the two samples of propolis showed different levels of inhibition on each of the enzymes tested. In general, EEP RS demonstrated a significantly higher inhibitory activity on GtfB and C activities (both solution and surface assays) than EEP MG at concentrations between 0.047 and 0.187 mg/ml (p<0.05). EEP MG, on the other hand, exhibited a greater inhibitory effect on the activities of surface GtfD (at 0.375, 0.75 and 1.5 mg/ml) and S. sanguis Gtf (at 1.5 and 3.0 mg/ml; p<0.05). These data indicate that EEP is a potent inhibitor of Gtf enzymes in solution and adsorbed on an experimental pellicle; however, its effect on Gtf activity is variable depending on the geographical origin of the propolis samples. There is a need to identify the active compounds of propolis.

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Investigation of Flavonoid Aglycones in Propolis Collected by Two Different Varieties of Bees in the Same Region

Cited by 49 publications

References 1 publication

Effectiveness of disinfectants on the adherence of <i>Candida albicans</i> to denture base resins with different surface textures

Effectiveness of disinfectants on the adherence of <i>Candida albicans</i> to denture base resins with different surface textures

Effects of nystatin, fluconazole and propolis on poly(methyl methacrylate) resin surface

Effects of <i>Apis mellifera</i> Propolis on the Activities of Streptococcal Glucosyltransferases in Solution and Adsorbed onto Saliva–Coated Hydroxyapatite

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