Investigation of Electromagnetic Resonance Rewarming Enhanced by Magnetic Nanoparticles for Cryopreservation

Pan, Jiaji; Ren, Shen; Sekar, Praveen K.; Ji, Peng; Shu, Zhiquan; Zhao, Gang; Ding, Weiping; Chen, Ming; Gao, Dayong

doi:10.1021/acs.langmuir.8b03060

Cited by 18 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gao and coworkers describe an EM rewarming method using a resonant cavity and use of both electric and magnetic rewarming to get high rates (>200 °C min −1 ) in tens of milliliters of cryopreserved samples, however, scale up to organs was not demonstrated. [ 36 ]…”

Section: Discussionmentioning

confidence: 99%

Vitrification and Nanowarming of Kidneys

et al. 2021

View full text Add to dashboard Cite

Vitrification can dramatically increase the storage of viable biomaterials in the cryogenic state for years. Unfortunately, vitrified systems ≥3 mL like large tissues and organs, cannot currently be rewarmed sufficiently rapidly or uniformly by convective approaches to avoid ice crystallization or cracking failures. A new volumetric rewarming technology entitled "nanowarming" addresses this problem by using radiofrequency excited iron oxide nanoparticles to rewarm vitrified systems rapidly and uniformly. Here, for the first time, successful recovery of a rat kidney from the vitrified state using nanowarming, is shown. First, kidneys are perfused via the renal artery with a cryoprotective cocktail (CPA) and silica-coated iron oxide nanoparticles (sIONPs). After cooling at −40°C min −1 in a controlled rate freezer, microcomputed tomography (μCT) imaging is used to verify the distribution of the sIONPs and the vitrified state of the kidneys. By applying a radiofrequency field to excite the distributed sIONPs, the vitrified kidneys are nanowarmed at a mean rate of 63.7°C min −1 . Experiments and modeling show the avoidance of both ice crystallization and cracking during these processes. Histology and confocal imaging show that nanowarmed kidneys are dramatically better than convective rewarming controls. This work suggests that kidney nanowarming holds tremendous promise for transplantation.

show abstract

Section: Discussionmentioning

confidence: 99%

Vitrification and Nanowarming of Kidneys

et al. 2021

View full text Add to dashboard Cite

show abstract

“…With IONPs distributed throughout the sample, rapid and uniform warming occurs, which eliminated cracking and ice formation and results in improved recovery and tissue viability . We note that hybrid heating methods, such as assisting electromagnetic heating with conduction heating and magnetic nanoparticles are also being investigated as potential solutions for large‐volume rewarming (Table S1, Supporting Information). However, the success was only limited to large‐volume (20 mL) cells suspensions so far, whereas nanowarming is theoretically fully scalable to L size organ systems 16b…”

Section: Introductionmentioning

confidence: 99%

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

et al. 2020

View full text Add to dashboard Cite

Cryopreservation technology allows long‐term banking of biological systems. However, a major challenge to cryopreserving organs remains in the rewarming of large volumes (>3 mL), where mechanical stress and ice formation during convective warming cause severe damage. Nanowarming technology presents a promising solution to rewarm organs rapidly and uniformly via inductive heating of magnetic nanoparticles (IONPs) preloaded by perfusion into the organ vasculature. This use requires the IONPs to be produced at scale, heat quickly, be nontoxic, remain stable in cryoprotective agents (CPAs), and be washed out easily after nanowarming. Nanowarming of cells and blood vessels using a mesoporous silica‐coated iron oxide nanoparticle (msIONP) in VS55, a common CPA, has been previously demonstrated. However, production of msIONPs is a lengthy, multistep process and provides only mg Fe per batch. Here, a new microporous silica‐coated iron oxide nanoparticle (sIONP) that can be produced in as little as 1 d while scaling up to 1.4 g Fe per batch is presented. sIONP high heating, biocompatibility, and stability in VS55 is also verified, and the ability to perfusion load and washout sIONPs from a rat kidney as evidenced by advanced imaging and ICP‐OES is demonstrated.

show abstract

“…After cooling and warming back, the viabilities of the sample with nanowarming ($90 C/min) were found to be much higher than that with conventional water-bath heating ($7 C/min). To achieve a high energy conversion while reducing the SPION dosages, Pan et al designed an electromagnetic warming system in which low concentrations of SPIONs were added into the CPA solution [129]. They reported the addition of SPIONs generated rapid warming of the bulk cryopreserved sample at >200 C min À1 .…”

Section: Nanowarmingmentioning

confidence: 99%

Magnetic Nanoparticle-Mediated Heating for Biomedical Applications

Kwizera

Stewart

Mahmud

et al. 2022

Journal of Heat Transfer

View full text Add to dashboard Cite

Magnetic nanoparticles, especially superparamagnetic nanoparticles (SPIONs), have attracted tremendous attention for various biomedical applications. Facile synthesis and functionalization together with easy control of the size and shape of SPIONS to customize their unique properties, have made it possible to develop different types of SPIONs tailored for diverse functions/applications. More recently, considerable attention has been paid to the thermal effect of SPIONs for the treatment of diseases like cancer and for nanowarming of cryopreserved/banked cells, tissues, and organs. In this mini-review, recent advances on the magnetic heating effect of SPIONs for magnetothermal therapy and enhancement of cryopreservation of cells, tissues, and organs, are discussed, together with the non-magnetic heating effect (i.e., high Intensity focused ultrasound or HIFU-activated heating) of SPIONs for cancer therapy. Furthermore, challenges facing the use of magnetic nanoparticles in these biomedical applications are presented.

show abstract

Investigation of Electromagnetic Resonance Rewarming Enhanced by Magnetic Nanoparticles for Cryopreservation

Cited by 18 publications

References 61 publications

Vitrification and Nanowarming of Kidneys

Vitrification and Nanowarming of Kidneys

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

Magnetic Nanoparticle-Mediated Heating for Biomedical Applications

Contact Info

Product

Resources

About