Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone

Özkunt, Okan; Sariyilmaz, Kerim; Gemalmaz, Halil Can; Gürgen, Seren Gülşen; Yener, Ulaş; Dikici, Fatih

doi:10.1177/2309499017739481

Cited by 9 publications

(9 citation statements)

References 26 publications

(39 reference statements)

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“…When compared with methylprednisolone, EPO seems to better increase the expression of PDGF-b and thus produce better function outcomes than methylprednisolone in SCI. 14 On the other hand, EPO could facilitate the recruitment of BMSCs to sites of SCI. 15 BDNF initiates several signal transduction pathways after binding with the BDNF receptor, suppressing the apoptosis of neurons.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Human Brain–Derived Neurotrophic Factor Gene-Modified Bone Marrow Mesenchymal Stem Cells Combined With Erythropoietin Can Improve Acute Spinal Cord Injury

Li¹,

Wang

Ding³

et al. 2020

Dose-Response

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Objective: To assess the effect as well as mechanism of bone marrow mesenchymal stem cells (BMSCs) modified by the human brain–derived neurotrophic factor gene combined with erythropoietin (EPO) in the treatment of acute spinal cord injury (SCI) in rats. Methods: The Brain-derived neurotrophic factor (BDNF) gene was transected by a virus vector. Rats with SCI were randomly split into following groups: The normal saline (NS) group, the EPO group, The Basso, Beattie, and Bresnahan scores, messenger RNA BDNF expression, and apoptosis rates were compared between the 4 groups at 1, 3, 7, 14, and 21 days after SCI. Results: At 7, 14, and 21 days after operation, the expression of the BDNF gene in the other 3 groups was higher than that of the NS group, and the difference was statistically significant ( P < .05). The apoptosis rate in the combined group was less than that of NS, EPO, and BDNF/BMSC groups, and the differences were statistically significant ( P < .05). Conclusion: Brain-derived neurotrophic factor gene-modified BMSC transplantation combined with EPO can promote the repair of nerve function after SCI in rats.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Human Brain–Derived Neurotrophic Factor Gene-Modified Bone Marrow Mesenchymal Stem Cells Combined With Erythropoietin Can Improve Acute Spinal Cord Injury

Li¹,

Wang

Ding³

et al. 2020

Dose-Response

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“…Thus, the single and immediate use of the catheter exempts it from being considered a confusion factor in the interpretation of the results. The experimental results found in this study reinforce previous studies 0 findings (22)(23)(24), which show the safety and reproducibility of the IR for research. However, these previous studies did not individually evaluate the IR in its entirety, considering motor, functional and histological aspects in the same assay.…”

Section: ' Resultssupporting

confidence: 90%

“…Furthermore, regarding the experiment 0 s time interval, the current work did not perform as in previous studies (19,20) that performed intradural punctures in different time phases after experimental SCI; it is possible that intradural puncture has different histological and functional repercussions depending on the temporal inflammatory phase in which the SCI is found. It is also this study 0 s limitation, the performance of intradural puncture only in the area of the SCI 0 s epicenter (24), differently from other authors (18) that published better functional recovery with intrathecal stem cells given distally to the anatomical site of SCI.…”

mentioning

confidence: 59%

“…The experimental rat model of SCI is well established (21), but the administration of drugs through the IR in this model is not. The standardization, evaluation, safety (22) and reproducibility of the IR for research have not yet been studied in full, considering the motor, functional and histological aspects (23,24). The present study aims to standardize the use of the IR in rats subjected to controlled experimental SCI.…”

Section: ' Introductionmentioning

confidence: 99%

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Standardization of an experimental model of intradural injection after spinal cord injury in rats

Letaif

Tavares-Júnior

Santos

et al. 2021

Clinics

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The intrathecal route has not yet been thoroughly standardized and evaluated in an experimental model of spinal cord injury (SCI) in Wistar rats. The objective of this study was to standardize and evaluate the effect of intradural injection in this animal model. METHOD: The animals were divided into 6 groups: 1) laminectomy and intradural catheter; 2) laminectomy, intradural catheter and infusion; 3) only SCI; 4) SCI and intradural catheter; 5) SCI, intradural catheter and infusion; and 6) control (laminectomy only). Motor evaluations were performed using the Basso, Beattie and Bresnahan (BBB) scale and the horizontal ladder test; motor evoked potentials were measured for functional evaluation, and histological evaluation was performed as well. All experimental data underwent statistical analysis. RESULTS: Regarding motor evoked potentials, the groups with experimental SCI had worse results than those without, but neither dural puncture nor the injection of intrathecal solution aggravated the effects of isolated SCI. Regarding histology, adverse tissue effects were observed in animals with SCI. On average, the BBB scores had the same statistical behaviour as the horizontal ladder results, and at every evaluated timepoint, the groups without SCI presented scored significantly better than those with SCI (po0.05). The difference in performance on motor tests between rats with and without experimental SCI persisted from the first to the last test. CONCLUSIONS: The present work standardizes the model of intradural injection in experimental SCI in rats. Intrathecal puncture and injection did not independently cause significant functional or histological changes.

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“…The experimental rat model of SCI is well established ( 21 ), but the administration of drugs through the IR in this model is not. The standardization, evaluation, safety ( 22 ) and reproducibility of the IR for research have not yet been studied in full, considering the motor, functional and histological aspects ( 23 , 24 ). The present study aims to standardize the use of the IR in rats subjected to controlled experimental SCI.…”

Section: Introductionmentioning

confidence: 99%

Standardization of an experimental model of intradural injection after spinal cord injury in rats

Letaif

Tavares-Júnior

Santos

et al. 2021

Clinics

View full text Add to dashboard Cite

OBJECTIVES: The intrathecal route has not yet been thoroughly standardized and evaluated in an experimental model of spinal cord injury (SCI) in Wistar rats. The objective of this study was to standardize and evaluate the effect of intradural injection in this animal model. METHOD: The animals were divided into 6 groups: 1) laminectomy and intradural catheter; 2) laminectomy, intradural catheter and infusion; 3) only SCI; 4) SCI and intradural catheter; 5) SCI, intradural catheter and infusion; and 6) control (laminectomy only). Motor evaluations were performed using the Basso, Beattie and Bresnahan (BBB) scale and the horizontal ladder test; motor evoked potentials were measured for functional evaluation, and histological evaluation was performed as well. All experimental data underwent statistical analysis. RESULTS: Regarding motor evoked potentials, the groups with experimental SCI had worse results than those without, but neither dural puncture nor the injection of intrathecal solution aggravated the effects of isolated SCI. Regarding histology, adverse tissue effects were observed in animals with SCI. On average, the BBB scores had the same statistical behaviour as the horizontal ladder results, and at every evaluated timepoint, the groups without SCI presented scored significantly better than those with SCI ( p <0.05). The difference in performance on motor tests between rats with and without experimental SCI persisted from the first to the last test. CONCLUSIONS: The present work standardizes the model of intradural injection in experimental SCI in rats. Intrathecal puncture and injection did not independently cause significant functional or histological changes.

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Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone

Abstract: EPO seems to better increase the expression of PDGF-β, thus produce better results in locomotor functions when compared to MP.

Cited by 9 publications

References 26 publications

RETRACTED: Human Brain–Derived Neurotrophic Factor Gene-Modified Bone Marrow Mesenchymal Stem Cells Combined With Erythropoietin Can Improve Acute Spinal Cord Injury

RETRACTED: Human Brain–Derived Neurotrophic Factor Gene-Modified Bone Marrow Mesenchymal Stem Cells Combined With Erythropoietin Can Improve Acute Spinal Cord Injury

Standardization of an experimental model of intradural injection after spinal cord injury in rats

Standardization of an experimental model of intradural injection after spinal cord injury in rats

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