Investigation of Drug Delivery by Iontophoresis in a Surgical Wound Utilizing Microdialysis

Holovics, Heidi J.; Anderson, Carter; Levine, Barry; Hui, Ho-Wah; Lunte, Craig E.

doi:10.1007/s11095-007-9490-2

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…In the present study microdialysis samples were collected for 5 h after removal of occlusion where as the decrease in IL-6 release was not possible to determine for longer time points. This can be overcome using return awake animal containment system (Holovics et al, 2008) where the microdialysis sampling can be carried out in conscious rats for extended period of time. These experiments are presently being pursued in our laboratory.…”

Section: Discussionmentioning

confidence: 99%

Dermal microdialysis of inflammatory markers induced by aliphatic hydrocarbons in rats

et al. 2009

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In the present study we made an attempt to understand the skin irritation cascade of selected aliphatic hydrocarbons using microdialysis technique. Microdialysis probes were inserted into dermis in the dorsal skin of hairless rats. After 2 h of probes insertion, occlusive dermal exposure (2 h) was carried out with 230 μl of nonane, dodecane and tetradecane, using Hill top chambers ® . Inflammatory biomarkers such as substance P (SP), α-melanocyte stimulating hormone (α-MSH) Interleukin 6 (IL-6) and prostaglandin E2 (PGE 2 ) were analyzed in the dialysis samples by enzyme immunoassay (EIA). SP, α-MSH and IL6 were released in significant amounts following the dermal exposure of nonane and dodecane, whereas tetradecane did not induce any of these markers in significant amounts compared to control. Nonane increased the PGE 2 levels in significant amounts within 2 h of chemical exposure compared to dodecane and tetradecane. IL-6 response was found to be slow and 2-3-fold increase in IL-6 levels was observed after 5 h following nonane and dodecane application. The magnitude of skin irritation exerted by all three chemicals was in the order of nonane ≥ dodecane ≥ tetradecane. The results demonstrate that microdialysis can be used to measure the inflammatory biomarkers in the skin irritation studies and irritation response of chemicals was quantifiable by this method. In conclusion, microdialysis was found to be an excellent tool to measure several inflammatory biomarkers as a function of time after dermal exposures with irritant chemicals.

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Section: Discussionmentioning

confidence: 99%

Dermal microdialysis of inflammatory markers induced by aliphatic hydrocarbons in rats

et al. 2009

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“…Although the primary applications of microdialysis sampling have been in the area of neuroscience research [1], it has also been extensively employed for other pharmaceutical applications. These include the investigation of the transdermal delivery of drugs [2], tissue pharmacokinetics [3], and regional metabolism of drugs in tissues [4,5]. …”

Section: Introductionmentioning

confidence: 99%

Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: A review

Nandi

Lunte

2009

Analytica Chimica Acta

198

150

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Microdialysis (MD) is a sampling technique that can be employed to monitor biological events both in vivo and in vitro. When it is coupled to an analytical system, microdialysis can provide near realtime information on the time-dependent concentration changes of analytes in the extracellular space or other aqueous environments. Online systems for the analysis of microdialysis samples enable fast, selective and sensitive analysis while preserving the temporal information. Analytical methods employed for online analysis include liquid chromatography (LC), capillary (CE) and microchip electrophoresis and flow-through biosensor devices. This review article provides an overview of microdialysis sampling and online analysis systems with emphasis on in vivo analysis. Factors that affect the frequency of analysis and, hence, the temporal resolution of these systems are also discussed.

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“…Using this approach, significantly different concentrations of the metabolites of a systemic administration of phenol were measured in the extracellular fluid of the anterior position compared to the other regions, even though the same amount of phenol was detected in each region. In more recent studies, linear probes have been implanted in specific layers of the skin (Holovics et al, 2008), stomach Lunte, 2008a, 2008b), and colon (Thomas, 2014).…”

Section: Linear Probementioning

confidence: 99%

Separation-Based Methods Combined With Microdialysis Sampling for Monitoring Neurotransmitters and Drug Delivery to the Brain

Saylor¹,

Thomas²,

Lunte³

2017

Compendium of in Vivo Monitoring in Real-Time Molecular Neuroscience

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Investigation of Drug Delivery by Iontophoresis in a Surgical Wound Utilizing Microdialysis

Abstract: The four microdialysis probe design was successful in the determination of localized drug penetration in a sutured incision model. Iontophoresis enhanced skin penetration and allowed for site specific delivery when applied to a sutured incision.

Cited by 11 publications

References 27 publications

Dermal microdialysis of inflammatory markers induced by aliphatic hydrocarbons in rats

Dermal microdialysis of inflammatory markers induced by aliphatic hydrocarbons in rats

Recent trends in microdialysis sampling integrated with conventional and microanalytical systems for monitoring biological events: A review

Separation-Based Methods Combined With Microdialysis Sampling for Monitoring Neurotransmitters and Drug Delivery to the Brain

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