Investigation of Coxiella burnetii Contamination in Commercial Milk and PCR Method for the Detection of C. burnetii in Egg

昭彦, 平井; 誠二, 金子; 晶子, 仲真; 直人, 石崎; 恵, 小田桐; 明美, 甲斐; 健志, 貞升; 敬行, 新開; 一好, 矢野; 聖, 諸角

doi:10.3358/shokueishi.46.86

Cited by 10 publications

(7 citation statements)

References 9 publications

(2 reference statements)

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“…The lowest detection limit in these studies was between 1 and 10 3 particles per milliliter in bovine milk [2,6,10,22] Campylobacter jejuni, C. coli, Escherichia coli and Listeria monocytogenes [1], Staphylococcus aureus [24], Brucella spp. [25] and Corynebacterium casei [17] have been found in dairy products by direct detection using PCR.…”

Section: Discussionmentioning

confidence: 81%

“…In Japan, 131 of 244 (53.7%) commercial milk samples tested by nested PCR were positive for C. burnetii [10]. Moreover, 14.3% of bovine milk samples tested by real-time PCR were positive for C. burnetii in Italy [19].…”

Section: Discussionmentioning

confidence: 99%

“…Extraction of DNA: The DNA extraction method described previously [10] was modified to enable efficient extraction from a cheese pellet. Each pellet was resuspended in 200 l of PBS.…”

Section: Methodsmentioning

confidence: 99%

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Development of a Method for Detecting Coxiella burnetii in Cheese Samples

et al. 2012

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ABSTRACT. Coxiella burnetii is the causative agent of Q fever, and the main route of infection in humans is inhalation of contaminated aerosols. Although oral transmission by contaminated raw milk or dairy products is also a possible route of human infection, there have been few studies investigating the presence of C. burnetii in dairy products. We developed a new method of extracting DNA from cheese and detecting C. burnetii DNA in cheese samples with a nested PCR assay. The limit of detection was 6.0  10 2 C. burnetii particles per gram. We subsequently used this method to examine the presence of C. burnetii in cheese at commercial markets in Tokyo from June 2005 to December 2008. Twenty-eight of 147 cheese samples were found to be positive for C. burnetii DNA. However, when we assessed the viability of C. burnetii by inoculating mice with DNA-positive samples, all of the samples were found to be negative. Thus, the viability of C. burnetii appears to have been lost in these cheese samples.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Development of a Method for Detecting Coxiella burnetii in Cheese Samples

et al. 2012

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“…The percentage of bovine milk samples testing positive in this study (41%; 95%CI: 20-62%), falls within the range of recent studies in Italy (27-60%) (Petruzzelli et al 2013;Vicari et al 2013), which used a touchdown PCR targeting the same IS1111 transposon-like repetitive region of the commercial kit used here. Investigations in other countries showed, however, quite variable positivity percentages, ranging from 83.8% in France (Guatteo et al 2007) to 66.7% in Hungary (Gyuranecz et al 2012), 57.1% in the United States (Loftis et al 2010), 53.7% in Japan (Hirai et al 2005) and 6.2% in Iran (Rahimi et al 2010).…”

Section: Resultsmentioning

confidence: 95%

Prevalence of Coxiella burnetii in cows’ and ewes’ bulk tank milk samples from selected dairy farms of Central Italy

Guidi

Petruzzelli

Ciarrocchi

et al. 2017

Italian Journal of Animal Science

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The prevalence of Coxiella burnetii, the causative agent of Q fever, in cattle and sheep raw milk farms was determined in Central Italy, an area in which dairy production plays an important economic role. Milk samples (n. 189), collected from 66 dairy farms in 2012-2013, were tested by a commercial real-time PCR assay. Seventeen dairy farms had at least one positive milk sample; percent positive was higher for cattle (50%) than sheep (21%) farms. Concerning milk, 15% of samples tested overall gave a positive result, with the highest percentage of positivity observed for bovine milk compared with sheep milk (41% and 12%, respectively). In the only bovine farm repeatedly sampled during the study, C. burnetii contamination was persistently found for almost a year. The prevalence calculated for the sheep farms showed a discontinuous trend with a maximum peak in February. The results obtained underline the widespread presence of the pathogen in the considered geographical area, giving new epidemiological information. Since the milk route of elimination is a potential vehicle of infection for farmers, veterinarians and for dairy stakeholders in general, BTM screening by real-time PCR can be applied as a useful surveillance tool both for the identification of infected flocks and implementation of control programmes.

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“…The results of investigation into this issue remain scarce, and there are reports of the PCR-based detection of Q-fever Coxiella DNA in dead bacterial fragments, but there are no reports demonstrating contamination with the viable bacteria. 39 However, sterilization of raw milk at 63°C for 30 min or more, which meets the sterilization condition for Q-fever Coxiella, is performed in Japan, as it is in the United States, and therefore no problem is expected regarding food contamination.…”

Section: Q Fever and Food Contaminationmentioning

confidence: 99%

Diagnosis and treatment of Q fever: attempts to clarify current problems in Japan

Watanabe

2008

Journal of Infection and Chemotherapy

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Investigation of Coxiella burnetii Contamination in Commercial Milk and PCR Method for the Detection of C. burnetii in Egg

Cited by 10 publications

References 9 publications

Development of a Method for Detecting Coxiella burnetii in Cheese Samples

Development of a Method for Detecting Coxiella burnetii in Cheese Samples

Prevalence of Coxiella burnetii in cows’ and ewes’ bulk tank milk samples from selected dairy farms of Central Italy

Diagnosis and treatment of Q fever: attempts to clarify current problems in Japan

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