Investigation of class-d beta-lactamases causing carbapenem resistance in clinical Acinetobacter baumannii isolates*

Davandeh, Iskandar; Eraç, Bayrı; Aydemir, Sabire

doi:10.3906/sag-1607-91

“…The primer ERIC-2 5′-AAGTAAGTGACTGGGGTGAGCG-3′ was used for amplification. PCR conditions were described previously [14]. The ERIC-PCR fingerprint of A. baumannii was analyzed using Tocan Gel analysis software (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Outbreak of carbapenem-resistant Acinetobacter baumannii carrying the carbapenemase OXA-23 in ICU of the eastern Heilongjiang Province, China

Zhang

¹

,

Hu

²

,

Zhang

³

et al. 2019

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Background To investigate the carbapenem resistance mechanisms and clonal relationship of carbapenem-resistant Acinetobacter baumannii (CRAB) strains isolated in the intensive care unit (ICU) of the First Affiliated Hospital of Jiamusi University, management approaches to ICU clonal CRAB outbreaks were described. Methods The sensitivity of the antibiotic was determined using the VITEK-2 automated system. Carbapenemase genes ( bla TEM , bla SHV , bla KPC , bla NDM , bla IMP-4 , bla VIM , bla OXA-23 , bla OXA-24 , bla OXA-51 , and bla OXA-58 ), AmpC enzyme genes ( bla ACC , bla DHA , bla ADC ), and ISAba1 were assessed for all collected isolates using polymerase chain reaction (PCR). The transfer of resistance genes was investigated via conjugation experiments. The clonal relationship of isolates was determined via enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus sequence typing (MLST). When the detection rate of CRAB increased from 25% in 2010 to 92% in 2014, a number of actions were initiated, including enhanced infection control, staff education, and the cleaning of the hospital environment. Results Clinical isolates were positive for the following genes: bla OXA23 , bla OXA51 , bla OXA24 , bla ADC , bla TEM , ISAba1 , ISA-23 , and ISA-ADC ; however, bla OXA58 , ISA-51 , bla NDM , bla IMP , bla KPC , bla TEM , bla SHV , bla VIM , and bla ACC were not detected. Four carbapenem-resistant isolates successfully transferred plasmids from A. baumannii isolates to E. coli J53. MLST showed that all strains belonged to ST2 except for one isolate, which belonged to the new genotype ST1199. The ERIC-PCR method fou...

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“…The genetic relationships among the 27 clinical A. baumannii isolates were determined by ERIC-PCR [ 24 ]. Primers' details are listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Dissemination and Genetic Relatedness of Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Isolates from a Burn Hospital in Iraq

Shali

¹

,

Jalal

²

,

Arif

³

2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

6

1

0

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Acinetobacter baumannii is an aggressive opportunistic bacterial pathogen that causes severe nosocomial infections, especially among burn patients. An increasing number of hospitals-acquired infections have been reported all over the world. However, little attention has been paid to the relatedness between A. baumannii isolates from different hospital environments and patients. In this study, 27 isolates were collected from the Burn and Plastic Surgery Hospital of Al Sulaymaniyah City, Iraq, from January through December 2019 (11 from patients and 16 from the wards environment), identified to species level as A. baumannii using Vitek 2 system and molecular detection of 16S rRNA gene, and then confirmed by targeting the blaOXA-51 gene. Moreover, the isolates were characterized by means of automated antimicrobial susceptibility assay, antimicrobial-resistant patterns, a phenotypic method using a combined disk test, and molecular methods for the detection of class A and C β -lactamase genes, and finally, the genetic relatedness was classified. Antimicrobial susceptibility testing showed that 63% (17/27) of the retrieved A. baumannii isolates were extensively drug-resistant to 8/9 antimicrobial classes. Furthermore, 37% (10/27) of the isolates were classified as multidrug-resistant; 8 isolates exhibited similar resistant patterns and the other two isolates showed 2 different patterns, while resistance was greater in isolates from patients than from the ward environment. Combined disk test showed that two isolates contained extended-spectrum β -lactamase. All isolates carried blaTEM-1, and two copies of the blaCTX-1 gene were indicated in one isolate, while blaSHV was absent in all isolates. Twenty-four isolates carried the blaAmpC gene; among them, 3 isolates harbored the insertion sequence ISAba-1 upstream to the gene. Using Enterobacterial Repetitive Intergenic Consensus PCR, the isolates were clustered into 6 distinct types; among them, two clusters, each of four strains, were classified to contain isolates from both patients and environments. The clusters of similar genotypes were found in inpatients as well as the environments of different wards during time periods, suggesting transmission within the hospital. Identification of possible infection sources and controlling the transmission of these aggressive resistance strains should be strictly conducted.

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“…OXA enzymes such as OXA-23, OXA-24/40, OXA-58, OXA-143 and OXA-235 are among the most prevalent in A. baumannii strains ( Table 2 ). OXA-23 was identified in Scotland [ 79 ], later disseminated globally, now reaching a high frequency in A. baumannii isolates [ 38 , 80 , 81 , 82 ]. Genes encoding OXA-type β -lactamases have been identified mainly chromosomally or plasmid located in A. baumannii strains [ 83 , 84 ].…”

Section: Short Characterization Of the Molecular Mechanisms Of Armentioning

confidence: 99%

Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii

Vrâncianu

¹

,

Gheorghe

²

,

Czobor

³

et al. 2020

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Antibiotic resistance is one of the biggest challenges for the clinical sector and industry, environment and societal development. One of the most important pathogens responsible for severe nosocomial infections is Acinetobacter baumannii, a Gram-negative bacterium from the Moraxellaceae family, due to its various resistance mechanisms, such as the β-lactamases production, efflux pumps, decreased membrane permeability and altered target site of the antibiotic. The enormous adaptive capacity of A. baumannii and the acquisition and transfer of antibiotic resistance determinants contribute to the ineffectiveness of most current therapeutic strategies, including last-line or combined antibiotic therapy. In this review, we will present an update of the antibiotic resistance profiles and underlying mechanisms in A. baumannii and the current progress in developing innovative strategies for combating multidrug-resistant A. baumannii (MDRAB) infections.

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Investigation of class-d beta-lactamases causing carbapenem resistance in clinical Acinetobacter baumannii isolates*

Cited by 11 publications

References 13 publications

Outbreak of carbapenem-resistant Acinetobacter baumannii carrying the carbapenemase OXA-23 in ICU of the eastern Heilongjiang Province, China

Outbreak of carbapenem-resistant Acinetobacter baumannii carrying the carbapenemase OXA-23 in ICU of the eastern Heilongjiang Province, China

Dissemination and Genetic Relatedness of Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Isolates from a Burn Hospital in Iraq

Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii

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