Investigation of Class 1 Integrons With Antibiotic Resistance Genes in Multidrug-Resistant Acinetobacter Baumannii Strains and Determination of Plant Extract Effects on Multidrug-Resistant Isolates

Saral, A.

doi:10.31925/farmacia.2021.5.13

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Detection of CTX -M ESBLs for isolate Acinetobacter baumannii 1(50%) of the isolate was positive for at least one CTX-M ESBLs gene, the frequency of CTX-M ESBLs gene from 2(100%) presence among Acinetobacter baumannii was as follows: bla CTX-M1,0(0%); bla CTX-M2, 1(50%); bla CTX-M9 0(0%) There was a significant association between the presences of ESBL genes .Our study is similar to these studies in in Saudi Arabia [36] carry this gene bla CTX-M2 (81%) and another study by [37] carry this gene bla CTX-M2 (43%).The agarose gel of PCR products was shown in Figure (3), (4). Pseudomonas aeruginosa isolate at product size.…”

Section: Molecular Detecting Antibiotic Resistance Genes In Gram Nega...supporting

confidence: 87%

Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

muslem¹,

Raheema²,

Yasir³

2022

World J. Bio. Pharm. Health Sci.

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Intensive care units (ICU) are epicenters for the emergence of antibiotic-resistant Gram negative bacteria and multi-resistant Gram positive infections, largely due to the inappropriate use of antimicrobials. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. The findings of the disc diffusion method used to test the isolates for ‎antibiotic susceptibility showed that Amikacin was the most effective ‎antibiotic against klebsiella pneumonia and pseudomonas aeruginosa. Phenotypically survey of ESBLs for all isolates were ESBL producers, of these, 13(68.4%) isolates ‎were determined as ESBLs-‎producers following confirmatory testing ‎to be ESBLs producers. Whereas, genotypically no isolate had ESBLs ‎producers CTX-M-type ESBL. The prevalence of TEM, SHV, and ‎OXA-1 were as follow: blaSHV 3(23 %). While blaTEM and blaOXA-‎‎1genes were absent among all isolates. Phenotypically survey of ‎ESBLs, for (20) isolates pseudomonas aeruginosa 20(100%) isolates were ESBLs ‎producers of these, 12(60%) isolates were determined as ESBLs-‎‎producers following confirmatory testing to be ESBLs producers. ‎While genotypically isolates were ESBL producers‏ ‏‎1(8.3%) of the ‎isolates were ESBLs producers CTX-M-type ESBL was the most ‎prevalent CTX-M2 was 1(8.3%) , CTX-M1 and CTXM9 were absent ‎among all isolates. The prevalence of TEM, SHV, and OXA-type1 were ‎as follow: blaSHV 4(33%). While blaTEM and blaOXA-1 genes were absent ‎among all isolates. ‎ Phenotypically survey of ESBLs, for (17) isolates E. coli ‎‎17(100%) isolates were ESBLs producers of these, 10(58.8%) isolates ‎were determined as ESBLs-‎producers following confirmatory testing ‎to be ESBLs producers. Whereas, Genotypically no isolate ESBLs ‎producers CTX-M-type ESBL, prevalence of TEM, SHV, and OXA-1 ‎were as follow: blaSHV 1(10%),blaOXA-1 1(10%) . While blaTEM gene was ‎absent among all isolates.

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Section: Molecular Detecting Antibiotic Resistance Genes In Gram Nega...supporting

confidence: 87%

Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

muslem¹,

Raheema²,

Yasir³

2022

World J. Bio. Pharm. Health Sci.

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“…The role and the most commonly found as drugs in curing various diseases are flavonoids, saponins and tannins. While in mobe leaves, based on research by Bhattacharya E et al in 2019, that the results of phytochemical screening in mobe leaf extracts contain secondary metabolites such as flavonoids, saponins and tannins [19]. Hakim et al research showed that the most abundant metabolites found in mobe plants were flavonoids [20].…”

Section: Table Imentioning

confidence: 99%

The Effects of the Combination of Ethanol Binahong Leaf (Anredera Cordifolia) and Mobe Leaf (Artocarpus Lakoocha) Extract Gel on Fibroblast and Osteocyte Proliferation in Wound Healing Post Tooth Extraction Socket on Wistar Rats (Rattus Norvegicus)

SATRIA¹

2022

FARMACIA

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Herbal medicines have been widely used throughout the world because of its fewer side effects compared to chemical-based medicines. The purpose of this study was to determine the effect of the combination of ethanol extract gel from binahong leaves and mobe leaves on the proliferation of fibroblasts and osteocytes in socket wounds after tooth extraction in Wistar rats. This in vivo experimental study involved 32 male Wistar rats which were randomly allocated to 8 groups of observations. Tooth extraction was carried out on the lower left incisor. A combined extract gel made of, binahong leaves and mobe leaves in the ratio of 1:1, 1:3, 3:1 was applied to group I to II, III to IV and V to VI respectively, meanwhile, group VII and VIII were given Aloclair ® gel and serve as the control group. On the 7 th and 21 st days after tooth extraction, the rats were sacrified and the proliferation of fibroblasts and osteocytes were observed with Hematoxylin-Eosin staining. Research data were analyzed using one-way ANOVA test. The results showed that the combination group of ethanol extract gel of binahong leaves and mobe leaves with a ratio of 3:1 had the highest number of fibroblast on 7 th day (p < 0.05) and the highest number of osteocyte on 21 st day compared to the control group (p < 0.05). The experiments showed that combination of ethanol binahong leaves and mobe leaves extract gel had an effect on increasing the proliferation of fibroblasts and osteocytes on the healing of socket wounds after tooth extraction in Wistar rats on the 7 th and 21 st days. RezumatMedicamentele pe bază de plante au fost utilizate pe scară largă în întreaga lume datorită efectelor secundare mai puține în comparație cu medicamentele pe bază de substanțe chimice. Scopul acestui studiu a fost de a determina efectul combinației de gel cu extract etanolic din frunze de Binahong și frunze de Mobe asupra proliferării fibroblastelor și osteocitelor în rănile de la nivelul soclului după extracția dinților la șobolanii Wistar. Acest studiu experimental in vivo a implicat 32 de șobolani Wistar masculi care au fost repartizați aleatoriu în 8 grupuri de observații. Extracția dentară a fost efectuată pe incisivul inferior stâng. Un gel de extract combinat format din frunze de binahong și frunze de mobe în proporție de 1:1, 1:3, 3:1 a fost aplicat la grupurile I -II, III -IV și, respectiv, V -VI, între timp, la grupurile VII și VIII s-a administrat gel Aloclair ® și a servit drept grup de control. În zilele 7 și 21 după extracția dinților, șobolanii au fost sacrificați și proliferarea fibroblastelor și a osteocitelor a fost observată cu colorarea cu Hematoxilină-Eozină. Rezultatele au arătat că grupul de combinație tratat cu gel de extract etanolic din frunze de binahong și frunze de mobe în raport de 3:1 a avut cel mai mare număr de fibroblaste în ziua a 7-a (p < 0,05) și cel mai mare număr de osteocite în ziua a 21-a în comparație cu grupul de control (p < 0,05). Experimentele au arătat că gelul cu extract etanolic din frunze de binahong și extract de fru...

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Antibiotics profile map of clinical A. baumannii strains isolated from health institutions in Turkey: a database search study and analysis of publications from 2011 to 2022

2023

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Background Acinetobacter baumannii is recognized as a major threat that causes healthcare-associated infections and causes a huge challenge to the health system worldwide. This research study was designed to detect the types and profiles of antibiotics tested against A. baumannii clinical strains in Turkey to evaluate their effectiveness and reevaluate their usage. Main body of the abstract The study depended on data search strategy using the online electronic database. We carried out a detailed analysis to all original research articles from 2011 to 2022 all conducted in Turkey. The study involved 91 articles and revealed about 40 antibiotics tested from 2006 to 2021 against A. baumannii with a different frequency. The more frequency antibiotics tested by health institutions in Turkey during this period included 15 antibiotics which are (Amikacin, Gentamicin, Imipenem, Meropenem, Cefoperazone–sulbactam, Ceftazidime, Cefepime, Ampicillin/sulbactam, Piperacillin, Piperacillin/tazobactam, Ciprofloxacin, Levofloxacin, Trimethoprim–Sulfamethoxazole, Colistin and Tigecycline). The frequency of resistance rate with percentage of (80–100%) shown by A. baumannii against these antibiotics was as follows (40.96%, 50.64%, 77.77%, 78.31%, 46.15%, 94.11%, 88.23%, 80.85%, 95.46%, 91.93%, 93.42%, 82.85%, 53.57%, 2.66%, 3.70%), respectively. From 2016 to 2021, an increase in resistance rates by A. baumannii against Colistin and Tigecycline was indicated noticeably. The 0% resistance rates during this period against Colistin were reported in a percentage of 16.6%, while the appearance of highly noticeable resistance (from 80 to 100 = 3.70%) against Tigecycline and the continuous elevation of resistance rates against this drug was worrisome. Short conclusion Stability in high resistance rates against some antibiotics for the last 10 years and the increase in resistance rates against effective antibiotics by A. baumannii should undergo for more studies and re-evaluation.

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Investigation of Class 1 Integrons With Antibiotic Resistance Genes in Multidrug-Resistant Acinetobacter Baumannii Strains and Determination of Plant Extract Effects on Multidrug-Resistant Isolates

Cited by 3 publications

References 19 publications

Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

The Effects of the Combination of Ethanol Binahong Leaf (Anredera Cordifolia) and Mobe Leaf (Artocarpus Lakoocha) Extract Gel on Fibroblast and Osteocyte Proliferation in Wound Healing Post Tooth Extraction Socket on Wistar Rats (Rattus Norvegicus)

Antibiotics profile map of clinical A. baumannii strains isolated from health institutions in Turkey: a database search study and analysis of publications from 2011 to 2022

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