Investigation of Bacterial Cellulose Biosynthesis Mechanism inGluconoacetobacter hansenii

Abstract

The present study explores the mechanism of cellulose biosynthesis in Gluconoacetobacter hansenii. The cellulose synthase enzyme was purified as membrane fraction and solubilized by treatment with 0.1% digitonin. The enzyme was separated by native-gel electrophoresis and β-D-glucan analysis was carried out using in vitro gel assay. The cellulose synthase has glycoprotein nature and composed two polypeptide subunits of 93 KDa and 85 KDa. The confirmation of β-1,4-glucan (cellulose) was performed in whole and hydrolyzed monomeric sugar form. Tinopal and Congo red were used for cellulose detection on the gel. Thus the in vitro cellulose synthesis assay with cell free enzyme fraction was attempted to improve the understanding of cellulose biosynthesis.

Production and characterization of bacterial cellulose by Leifsonia sp. CBNU-EW3 isolated from the earthworm, Eisenia fetida

“…The fraction Iα and Iβ mixture of allomorphs was attributed to the cultivation condition of the bacteria and also the ratio of cellulose, which may differ according to bacterial strain. All of these results were consistent with earlier published X-ray data for BC obtained with other bacteria [7,25,26,[29][30][31][32][33][34]. However, compared with other bacteria, the peaks of BC produced by Leifsonia sp.…”

“…In addition, the absorption bands at 1650, 1560, 1424, and 1060/cm were attributed to C-H stretching of -CH 2 , O-H bending of adsorbed water, -CH 2 symmetric bending and C-O-C pyranose ring skeletal vibrations, respectively [3,7,[24][25][26][27]. According to studies by Nelson & Connor [28] and Bhavna et al [29], the weak, broad band at 891/cm and strong band centered at 1424 cm -1 in the spectra of microbial cellulose correlates with CH 2 scissoring, which is highly indicative of cellulose. By correlating the FT-IR spectrum described above with previous literature on BC production, we confirmed that the pellicle obtained from Leifsonia sp CBNU-EW3 was indeed bacterial cellulose.…”

Peptide transporter YjiY influences the expression of the virulence gene mgtC to regulate biofilm formation in Salmonella

“…The stability of a biofilm depends on the EPS, which help in aggregation of cells, absorption of nutrients, stabilization of the architecture of the biofilm and cell to cell communication (Flemming and Wingender 2010). EPS can be stained with Congo red, which binds to cellulose and curli fimbriae, the two major components of Salmonella EPS (Romling et al 2000;Mohite and Patil 2014). We checked the distribution of EPS to measure the extent of biofilm formed on the glass coverslip.…”

Controllable Fabrication of BC Based on Time Growth

“…[18,19] Due to statistics, a number of new developments in the biological and cytological aspects of cellulose biosynthesis from Acetobacter have led to better understanding of this process. [19] Cellulose biosynthesis is an exciting area of investigation with lots of challenges and opportunities. [19,20] .…”

“…[19] Cellulose biosynthesis is an exciting area of investigation with lots of challenges and opportunities. [19,20] . (1d, 2d, 10d).…”