Investigation of antioxidant compounds in commercial pomegranate molasses products using matrix-solid phase dispersion extraction coupled with HPLC

Kamal, YT; Alam, Prawez; Alqasoumi, Saleh I.; Foudah, Ahmed I.; Alqarni, Mohammed H.; Yusufoğlu, Hasan Soliman

doi:10.1016/j.jsps.2018.03.015

Cited by 17 publications

(4 citation statements)

References 11 publications

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“…The results of Eyigün [34] were consistent with our results, while the results of Kamal et al [35] were found to be higher. This may be due to differences in raw material, production parameters and analysis methods.…”

Section: Resultssupporting

confidence: 92%

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Bazı nar ürünlerinin antioksidan özellikler ve in-vitro biyoerişilebilirlik açısından değerlendirilmesi

İncedayı

2021

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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In this research, pomegranate molasses, pomegranate sour sauces and pomegranate jams were evaluated in terms of some physicochemical properties including hydroxymethylfurfural, total phenolic content, antioxidant capacity and bioaccessibility. While ascorbic acid of these products ranged between 0.89-19.78 mg 100g-1 , total phenolic contents changed between 31.40-2061.10 mg gallic acid equivalent 100g-1. Antioxidant capacities of the products were determined as 34.01-2377.52 mg trolox equivalent 100g-1 with DPPH assay and 18.9-6439.0 mg trolox equivalent 100g-1 with CUPRAC assay. The bioaccessibilities regarding phenolic substance and antioxidant capacity after simulated gastrointestinal digestion ranged between 74-247% and 53-213%, respectively. High HMF levels, which were reflected on color and sensory features, have indicated the necessity of improving the production and storage conditions. On the other hand, the highest ascorbic acid, total phenolic content and antioxidant capacity of pomegranate molasses and the bioaccessibility of pomegranate jams in terms of antioxidants showed the importance of consumption of these products in the daily diet. So, this study can be regarded as a case surveillance study that can be used by producers, nutritionals and authorities to make assessments on manufacturing conditions, consumer health and nutrition.

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Section: Resultssupporting

confidence: 92%

“…The high HMF content of PJ 1 refers to inappropriate process conditions and this could cause a loss in ascorbic acid content. Eyigün [34] and Kamal et al [35] determined the ascorbic acid contents of PM samples between 0.02-0.19 g L -1 and 0.154-0.250 g 100g -1 respectively.…”

Section: Resultsmentioning

confidence: 99%

Bazı nar ürünlerinin antioksidan özellikler ve in-vitro biyoerişilebilirlik açısından değerlendirilmesi

İncedayı

2021

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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“…Components of pomegranate include punicalagin [ 41 ], corilagin [ 42 ], vitamin C, gallic acid, rutin, and ellagic acid [ 61 ]. Pomegranate is considered to be very promising for the prevention of variable malignancies [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Discovery of cancer-preventive juices reactivating RB functions

Masuda,

Horinaka,

Yasuda

et al. 2023

Environ. Health Prev. Med.

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Background Recent advances have been achieved in the genetic diagnosis and therapies against malignancies due to a better understanding of the molecular mechanisms underlying carcinogenesis. Since active preventive methods are currently insufficient, the further development of appropriate preventive strategies is desired. Methods We searched for drinks that reactivate the functions of tumor-suppressor retinoblastoma gene (RB) products and exert anti-inflammatory and antioxidant effects. We also examined whether lactic acid bacteria increased the production of the cancer-specific anti-tumor cytokine, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), in human, and examined whether the RB-reactivating drinks with lactic acid bacteria decreased azoxymethane-induced rat colon aberrant crypt foci (ACF) and aberrant crypts (ACs) in vivo . Results Kakadu plum juice and pomegranate juice reactivated RB functions, which inhibited the growth of human colon cancer LIM1215 cells by G1 phase arrest. These juices also exerted anti-inflammatory and antioxidant effects. Lactiplantibacillus ( L.) pentosus S-PT84 was administered to human volunteers and increased the production of TRAIL. In an in vivo study, Kakadu plum juice with or without pomegranate juice and S-PT84 significantly decreased azoxymethane-induced rat colon ACF and ACs. Conclusions RB is one of the most important molecules suppressing carcinogenesis, and to the best of our knowledge, this is the first study to demonstrate that natural drinks reactivated the functions of RB. As expected, Kakadu plum juice and pomegranate juice suppressed the growth of LIM1215 cells by reactivating the functions of RB, and Kakadu plum juice with or without pomegranate juice and S-PT84 inhibited rat colon ACF and ACs. Therefore, this mixed juice has potential as a novel candidate for cancer prevention. Supplementary information The online version contains supplementary material available at https://doi.org/10.1265/ehpm.23-00160 .

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“…Citric acid constitutes almost 1.19 g/100 g. In a different study, HPLC-MS was used in order to quantitatively investigate the total acidity in terms of citric acid between authentic and commercial pomegranate molasses [27]. Kamal and his team used matrix solid-phase dispersion extraction coupled with HPLC to study the composition of antioxidants in commercial pomegranate, which included vitamin C, gallic acid, rutin, and ellagic acid [28]. It was published elsewhere that sugars such as fructose, glucose, and sucrose in sugarcane molasses were determined using HPLC with a refractive index detector [29].…”

Section: Introductionmentioning

confidence: 99%

A Rapid HPLC Method for the Simultaneous Determination of Organic Acids and Furans: Food Applications

et al. 2022

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Concerns over the potential adulteration of commercially produced foods give rise to a requirement for a simple and fast analytical method capable of quantifying potential adulterants. This work demonstrates a simple HPLC method tailored to detect major organic acids and furans within ingredients in commercial food products, for example, pomegranate molasses, balsamic vinegar, and apple cider vinegar. The relative importance of this method is in its simplicity and its use of an environmentally friendly aqueous mobile phase under isocratic conditions, providing results in a less than 20 min runtime. The chromatographic separation was achieved using an Acclaim® OA, 5 µm, 120 Å (4.0 × 250 mm) column; a UV-DAD detector set at 210 nm; and a 200 mM Na2SO4 mobile phase with 0.55 mL/L methanosulfonic acid as a pH modifier. The method was then validated by quantifying the concentration of acetic acid, formic acid, citric acid, and hydroxymethyl furfural (HMF) in pomegranate molasses, balsamic vinegar, and apple cider vinegar commercial products. The concentration of acetic acid and HMF in balsamic vinegar was 80.380 mg/mL (±1.272 mg/mL) and 2.153 mg/mL (±0.021 mg/mL), respectively. The apple cider vinegar was composed only of acetic acid with a concentration of 44.139 mg/mL (±0.053 mg/mL). The concentrations of citric acid and HMF were 123.425 mg/mL (±2.502 mg/mL) and 11.382 mg/mL (±0.582 mg/mL), respectively, in pomegranate molasses. Furthermore, this method is also capable of determining various organic acids and furans in biomass: levulinic acid, formic acid, furfurals, diformylfuran, and gamma-valerolactone.

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Investigation of antioxidant compounds in commercial pomegranate molasses products using matrix-solid phase dispersion extraction coupled with HPLC

Cited by 17 publications

References 11 publications

Bazı nar ürünlerinin antioksidan özellikler ve in-vitro biyoerişilebilirlik açısından değerlendirilmesi

Bazı nar ürünlerinin antioksidan özellikler ve in-vitro biyoerişilebilirlik açısından değerlendirilmesi

Discovery of cancer-preventive juices reactivating RB functions

A Rapid HPLC Method for the Simultaneous Determination of Organic Acids and Furans: Food Applications

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