Investigation of antihyperalgesic and antiedematous activities of three Hieracium species

Abstract

Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α-and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50-200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%-56.2% (ED 50 =163.0±26.5 mg/kg) and 25.3%-51.6% (ED 50 =211.6±70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.

Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones

“…The plant material and extraction procedure were given previously in Milutinović et al [2,7] . Namely, the flowering aerial parts of investigated Hieracium species were collected on Mt Durmitor (NW Montenegro): H. calophyllum and H. coloriscapum in August 2013, H. scheppigianum and H. glabratum in August 2014, and H. pseudoschenkii and H. valdepilosum in August 2015; voucher numbers H05 ( H. scheppigianum ), H06 ( H. glabratum ), H10 ( H. coloriscapum ), H14 ( H. pseudoschenkii ), H19 ( H. valdepilosum ) and H27 ( H. calophyllum ) (Herbarium of the Natural History Museum in Belgrade, BEO).…”

“…It was demonstrated recently that all five MeOH extracts exhibited strong antioxidant activity in FRAP (ferric reducing antioxidant power), DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and 2‐deoxyribose assays, [2] while H. glabratum and H. calophyllum MeOH extracts and H. scheppigianum CH 2 Cl 2 extract showed significant antihyperalgesic activity in rats [7] …”

“…GC‐FID and GC/MS method was previously applied for the analysis of triterpenes (using commercial standards and their semi‐synthetized acetates) in CH 2 Cl 2 extract of H. sheppigianum flowering aerial parts. It was shown that this extract contained notable amounts of triterpenes of ursane type, α ‐amyrin and its acetate (81.0 and 29.0 mg/g), followed by those of oleane type, β ‐amyrin and its acetate (10.4 and 3.0 mg/g), and lupane type, lupeol acetate (8.0 mg/g) [7] …”

“…Moreover, for only about 30 Hieracium s. str. species pharmacological activities were tested [2–8] . Regarding antimicrobial and cytotoxic activities, only H. umbellatum L. and H. waldsteinii Tausch extracts were investigated.…”

“…flowering aerial parts , and dried dichloromethane (CH 2 Cl 2 ) extract of H. scheppigianum FREYN flowering aerial parts. The extracts were selected based on their composition ( Table S1 ) among previously chemically characterized MeOH and CH 2 Cl 2 flowering aerial parts extracts of 28 Hieracium species from Balkan Peninsula [2,7,12] …”