Investigation of Anti-oxidative and Anti-genotoxic Effects of<i>Origanum vulgare</i>L. Essential Oil on Human Lymphocytes<i>In vitro</i>

Çeker, Selçuk; Ağar, Güleray; Nardemir, Gokce; Anar, Mustafa; Kızıl, Hamit Emre; Alpsoy, Lokman

doi:10.1080/0972060x.2012.10662604

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…Drugs of herbal origin have been used in traditional systems of medicine since ancient times, and many researchers have benefited from medicinal plants to prevent oxidative stress. [ 13 14 15 16 17 ]…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

et al. 2015

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Background:Mushrooms have been valued for their nutritive content and as traditional medicines; several important medicinal properties of mushrooms have been recognized worldwide.Objective:The purpose of this study was to elucidate the cell growth inhibitory potential of four edible mushrooms; Coprinus comatus (O.F. Mull.) Pers. (Agaricaceae), Tricholoma fracticum (Britzelm.) Kreisel (Tricholomataceae), Rhizopogon luteolus Fr. and Nordholm (Rhizopogonaceae), Lentinus tigrinus (Bull.) Fr. (Polyporaceae) on hepatocellular carcinoma (HepG2) cells in conjunction with their antioxidant and antibacterial capacities.Materials and Methods:Five different extracts of edible mushrooms were obtained using water, methanol, acetone, n-hexane and chloroform as solvent systems for cytotoxic, antioxidant and antibacterial properties.Results:C. comatus showed substantial in vitro cytotoxic activity against HepG2 cell lines with all extracts especially with chloroform 50% inhibition (IC50 value of 0.086 mg/ml) and acetone (IC50 value of 0.420 mg/ml). Chloroform extract of C. comatus had maximum amount of β-carotene (25.94 μg/mg), total phenolic content (76.32 μg/mg) and lycopene (12.00 μg/mg), and n-hexane extract of L. tigrinus had maximum amount of flavonoid (3.67 μg/mg). While chloroform extract of C. comatus showed the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) capturing activity (1.579 mg/ml), the best result for metal chelating activity was obtained from methanolic extract (0.842 mg/ml). Moreover, all tested mushrooms demonstrated antibacterial activity and n-hexane extract of L. tigrinus and acetone extracts of T. fracticum were the most active against tested microorganism.Conclusion:These results indicate that different extracts of investigated mushroom have considerable cytotoxic, antioxidant and antibacterial properties and may be utilized as a promising source of therapeutics.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

et al. 2015

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“…Moreover, essential oils isolated from Origanum vulgare L. ssp. vulgare (Lamiaceae) showed significant antimutagenic activities against genotoxic effects of aflatoxin B 1 19 …”

Section: Resultsmentioning

confidence: 99%

“…Many compounds and complex mixtures such as food additives, beverages and insecticides have been tested by SMART 15 . Thus, there are many studies in the literature about the interactions of mutagens with various plants deciphered by micronucleus 16‐19 and SMART assays 14,20 …”

Section: Introductionmentioning

confidence: 99%

“…14 Many compounds and complex mixtures such as food additives, beverages and insecticides have been tested by SMART. 15 Thus, there are many studies in the literature about the interactions of mutagens with various plants deciphered by micronucleus [16][17][18][19] and SMART assays. 14,20 Hydrogen peroxide (H 2 O 2 ) is a well-known inducer of cancer in animals because of its mutagenic potential and it is a natural source of oxidative damage in cells, causing a spectrum of DNA lesions, including single-and double-strand breaks.…”

Section: Introductionmentioning

confidence: 99%

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Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

et al. 2022

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BACKGROUND Plants have important chemical compounds to protect organisms from many mutagens but they may also have dangerous toxic substances that must be tested before use. Lallemantia iberica has been used by local people for treatment of some diseases but it was never tested for its cell protective and antimutagenic/antirecombinogenic properties. In this study, it was aimed to determine the cytoprotective, antimutagenic/antirecombinogenic, antibacterial, antioxidant and phenolic profile of L. iberica by MTS assay, micronucleus test/somatic mutation recombination test (SMART), disc diffusion method, ABTS/DPPH tests and liquid chromatography (LC), respectively. RESULTS MTS assay results showed that both of the extracts supported the normal growth of healthy human lymphocytes and there was no significant difference between extracts. Although there was no significant increase in micronucleus concentration (‰) of the cultures treated with ethanol or methanol extracts, methanol extract caused slightly lower micronucleus concentration (12.64 ± 3.65‰) than the ethanol extract (24.46 ± 4.50‰). SMART results showed that ethanol and methanol extracts prevented harmful mitotic recombination. Lallemantia iberica also showed antibacterial activity against all of the strains tested, and the largest inhibition zones were observed with Pseudomonas aeruginosa (17.9 mm) and Enterobacter aerogenes (13.8 mm). Methanol extracts showed better antioxidant activities (ABTS IC50: 104.07 μg mL−1, DPPH IC50: 58.8 μg mL−1) than ethanol extracts (ABTS IC50: 344.03 μg mL−1, DPPH IC50: 233.7 μg mL−1). According to the LC results, the most abundant phenolic compounds were rutin hydrate (1796.4 ± 62.9 mg kg−1) and p‐coumaric acid (228.1 ± 7.60 mg kg−1). CONCLUSION Lallemantia iberica extracts had cell‐protective, antimutagenic/antirecombinogenic, antibacterial and strong antioxidant characteristics in relation to their rich phenolic content. © 2022 Society of Chemical Industry.

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Protective effects of three luteolin derivatives on aflatoxin B1-induced genotoxicity on human blood cells

et al. 2016

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Investigation of Anti-oxidative and Anti-genotoxic Effects ofOriganum vulgareL. Essential Oil on Human LymphocytesIn vitro

Cited by 7 publications

References 37 publications

Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties

Cytoprotective, antimutagenic/antirecombinogenic and antibacterial properties of Lallemantia iberica extracts

Protective effects of three luteolin derivatives on aflatoxin B1-induced genotoxicity on human blood cells

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