Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study

Abstract

Aloe vera is one of the medicinal plants whose importance has been better understood recently with its antibacterial, antimicrobial, antioxidant and anticancer properties. Although it is known that the aloe vera family has protective effects on neurons, the neuroprotective effect of the aloe barbadensis miller plant has not yet been fully explained. Glufosinate is structurally similar to glutamate and is an herbicide that blocks glutamine synthesis. Glutamate has been shown to induce cyclooxygenase-2 (COX-2), which causes toxicity. In the present study, neuron culture was exposed to Aloe vera barbadensis Miller plant extracts plant (25, 50, 100, 200, 400, 800, and 1600 µgr/ml doses) for 24 hours to protect against glufosinate (200 mM) and glutamate (10 -5 mM) toxicity. After 24 hours, MTT, TAC, and TOS analyzes were performed and the results were revealed. In our study, it was seen that the aqueous extract of aloe barbadensis miller plant, glufosinate, and glutamate, could preserve the vitality of neurons (89% protection in AVB 400 µgr/ml group). At the same time, it was seen that while increasing the antioxidant level in neurons, it decreased the oxidant level. The group that increases the antioxidant value best is AVB 400 µgr/ml (the group that increases it 1.4 times). When the findings were evaluated, it was concluded that aloe vera and its components may have a neuroprotective effect.

Cancer Suppression by Lymphocytes Activated by Cancer-Mediated Exosomes: an In Vitro Study

“…For the second absorbance measurement, 75 μL Reagent 2 solution was added to the same wells, and measurement was made at 660nm after 10 minutes. Absorbance values were applied according to the formula below and antioxidant values were calculated as Trolox Equiv mmol/L -1 [14].…”