Investigation of 3D silvernanodendrite@glass as surface-enhanced Raman scattering substrate for the detection of Sildenafil and GSH

Lv, Meng; Gu, Hui; Yuan, Xiaocong; Gao, Jin-Ying; Cai, T.

doi:10.1016/j.molstruc.2012.07.002

Cited by 9 publications

(9 citation statements)

References 49 publications

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“…Meanwhile, the bands originating from the N-terminal group are only present at picomolar concentrations for the 532 nm Raman laser irradiation in the spheroidal-cuboidal nanonetwork. This weak band at 1055 cm -1 is first assigned to the amino group, suggesting that GSH interacts with the nickel nanonetwork and not only through the carboxylate group, as suggested earlier by Meng Lv et al 177 , but other functional groups may also interact with Ni/NiO. The band appearing at 1633 cm -1 can be assigned to the first amide vibration.…”

Section: A) B) C)supporting

confidence: 65%

Hybridizing nickel platform using ultra-short pulsed laser: a new paradigm towards biomedical sensing and theranostics

Vijayakumar¹

2021

Preprint

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A self-assembled 3D nanonetwork of Nickel and Nickel Oxide is synthesized by ultrashort pulsed laser through multiphoton ionization. The synthesized nanonetwork with tunable physiochemical property was investigated for cancer therapeutic and biomolecular sensing applications. In this thesis, the developed 3D nickel nanomatrix effectively regulated HeLa cancer cell adhesion and proliferation mimicking Extracellular Matrix (ECM). This behaviour explicitly demonstrated that the initial incubation period was devoted to baiting fibroblast and HeLa cells to proliferate upon the nanomatrix and subsequently the same nanomatrix exhibited cell trapping behaviour upon HeLa cells after an increased incubation period thereby controlling proliferation. The results brought new insight as to how HeLa cells behaved differently when compared to NIH3T3 fibroblast cells opening pioneering application in drug-free cancer therapy. To delve deeper into nickel nanonetwork for cancer therapy the laser ionization was manipulated to induce two distinct quantum theranosomes. Presently, quantum materials are limited due to 0D & 1D materials lacking biocompatibility resulting in coated materials with labelled tags for fluorescence excitation. The theranosomes mimicked tumor microenvironment by selectively accelerating the proliferation of mammalian fibroblasts cells while inducing cancer therapy. Furthermore, the theranosomes opened up label-free bioimaging probe for differentiating (HeLa & MDAMB-231) from mammalian fibroblast cells for cancer diagnostics. In-addition to label-free bioimaging, the development of an ultrasensitive biosensor for targeted biomolecule sensing was developed addressing the drawback faced with fluorescence imaging using Surface Enhanced Raman Scattering (SERS). We developed a SERS active nano-biosensor to detect chemical dye Crystal Violet (CV) and biomolecule glutathione(GSH). The Raman detection of crystal violet (CV) and glutathione (GSH) molecules was noted with 1 pM (1×10-12M) concentrations at (532 & 785nm) excitation wavelengths with an enhancement factor of 109, not been observed even in plasmonic materials. This extends the limit of detection (LOD), confirming suitability for chemical and biomolecular sensing. Additionally, the quantum confinement effect will result in an ultrasensitive sensor diagnosing and differentiating cancer cells from fibroblast cells. Based on the results in this thesis, the multifunctional feasibility of nano and quantum scale nickel structures arranged in 3D assembly for its direct application in cancer therapeutics, encompassing cancer bioimaging and diagnostics.

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Section: A) B) C)supporting

confidence: 65%

Hybridizing nickel platform using ultra-short pulsed laser: a new paradigm towards biomedical sensing and theranostics

Vijayakumar¹

2021

Preprint

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“…3 and the assignments are shown in Table 1. 33 Fortunately, this C-S vibration has almost the identical Raman intensity for both GSH and GSSG per unit molar (Fig. S4 †) and noticed that Raman spectra could be inuenced by many other factors under different under different conditions.…”

Section: Sers Identication Of Gsh and Gssgmentioning

confidence: 88%

“…For the spectra of GSH and GSSG, the most prominent band occurs at 657 cm À1 and 659 cm À1 , respectively, which is attributed to the C-S stretching vibration. 33 Fortunately, this C-S vibration has almost the identical Raman intensity for both GSH and GSSG per unit molar (Fig. S8 †), so we can use this band as an internal reference for normalization of the spectra, and as will be explained in the following section, this property can also facilitate us to give the quantitative evaluation of the GSH/GSSG mixture samples.…”

Section: Sers Identication Of Gsh and Gssgmentioning

confidence: 98%

A SERS study of oxidation of glutathione under plasma irradiation

Huang

2015

RSC Adv.

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This paper reports a new application of surface enhanced Raman scattering (SERS) in analysis of oxidation of glutathione (GSH) to oxidized glutathione (GSSG), an important biochemical redox reaction in biological systems, under oxidative stress imposed by dielectric barrier discharge (DBD). Using the silver nanoparticles (NPs) prepared through the reduction of AgNO 3 by beta-cyclodextrin (b-CD), the transformation of GSH to GSSG under DBD irradiation can be probed with only a small quantity of sample at low concentration. Based on the intensity ratio of two characteristic Raman bands, i.e., the band at 1051 cm À1 (C-N stretching) and the band at 509 cm À1 (S-S stretching), which stem respectively from GSH and GSSG, the conversion between the reduced and oxidized glutathione can be determined quantitatively. This work demonstrates another useful extension of the SERS technique applied to bioscience research, i.e., rapid probing and quantitative assessing of chemical reactions of biomolecules under oxidative stress conditions.

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“…29 Interestingly, the recorded SERS spectra of GSH in aqueous standards and SERS spectra of WB samples were highly similar and in good agreement with previous results. Lv et al 24 reported that the Raman spectrum of GSH adsorbed to a 3D silver nanodendrite@glass film with bands at 791, 722 and 656 cm −1 . Based on the Raman spectra reported for the component amino acids of GSH, the bands at 722 and 656 cm −1 were associated with the vibrations of the sulfur atom of the amino acid, Cys.…”

Section: Raman and Sers Spectra Of Gsh And Blood Samplesmentioning

confidence: 99%

“…On the one hand side SERS detection of GSH using reversed reporting agents has been proposed for aqueous GSH solutions 19 as well as intracellular GSH 20,21 and GSH in WB. 21 The direct determination of GSH in aqueous solutions using SERS has been proposed repeatedly [22][23][24] and very recently SERS using Ag NPs has been proposed for the monitoring of GSH oxidation in plasma samples under non-thermal irradiation. 25 However, to the best of our knowledge, direct measurements in WB samples have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Surface enhanced Raman spectroscopic direct determination of low molecular weight biothiols in umbilical cord whole blood

Kuligowski

El-Zahry

Sánchez-Illana

et al. 2016

Analyst

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Biothiols play an essential role in a number of biological processes in living organisms including detoxification and metabolism. Fetal to neonatal transition poses a pro-oxidant threat for newborn infants, especially those born prematurely. A reliable and rapid tool for the direct determination of thiols in small volume whole blood (WB) samples would be desirable for its application in clinical practice. This study shows the feasibility of Surface Enhanced Raman Spectroscopy (SERS) using a silver colloid prepared by reduction of silver nitrate using hydroxylamine, as the SERS substrate for the quantification of thiols in WB samples after a simple precipitation step for protein removal. Bands originating from biothiols (790, 714 and 642 cm(-1)) were enhanced by the employed SERS substrate and the specificity of the detected SERS signal was tested for molecules presenting -SH functional groups. A statistically significant correlation between the obtained SERS signals and the thiol concentration measured using a chromatographic reference method in umbilical cord WB samples could be demonstrated. Using WB GSH concentrations obtained from the chromatographic reference procedure, a Partial Least Squares (PLS) regression model covering GSH concentrations from 13 to 2200 μM was calculated obtaining a root mean square error of prediction (RMSEP) of 381 μM when applied to an external test set. The developed approach uses small blood sample volumes (50 μL), which is important for clinical applications, especially in the field of neonatology. This feasibility study shows that the present approach combines all the necessary characteristics for its potential application in clinical practice.

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Investigation of 3D silvernanodendrite@glass as surface-enhanced Raman scattering substrate for the detection of Sildenafil and GSH

Cited by 9 publications

References 49 publications

Hybridizing nickel platform using ultra-short pulsed laser: a new paradigm towards biomedical sensing and theranostics

Hybridizing nickel platform using ultra-short pulsed laser: a new paradigm towards biomedical sensing and theranostics

A SERS study of oxidation of glutathione under plasma irradiation

Surface enhanced Raman spectroscopic direct determination of low molecular weight biothiols in umbilical cord whole blood

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