Investigation by bioassay of the efficacy of sodium hydroxide treatment on the inactivation of mouse-adapted scrapie

Ünal, Arzu; Thyer, James M.; Uren, Eric; Middleton, DJ; Braun, Maya; Maher, D.

doi:10.1016/j.biologicals.2006.08.003

Cited by 10 publications

(8 citation statements)

References 15 publications

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“…The majority of studies investigating prion inactivation by NaOH, conducted with high concentrations of prion‐infected animal brain tissue preparations (crude brain materials, slices of brain, 10% BH), obtained significant inactivation at only high alkali concentrations (≥1 mol/L) . Our results confirmed more recent studies that showed higher inactivating efficacy of low NaOH concentrations (0.025 to 0.5 mol/L NaOH) on diluted BH concentrations (spiking material, 0.125% to 1%) …”

Section: Discussionsupporting

confidence: 89%

“…Several studies suggest that low concentrations of NaOH (≤1 mol/L) may inactivate prions when low percentage (≤1%) of BH is used as infectious material . We further explored this relationship by first evaluating PrP Sc levels in various concentrations (0.2, 1, 5, and 10%) of 263K‐infected BH treated or not with increasing concentrations of NaOH (0.02‐0.5 mol/L).…”

Section: Resultsmentioning

confidence: 99%

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Decontamination of prions in a plasma product manufacturing environment

et al. 2013

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Section: Discussionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Decontamination of prions in a plasma product manufacturing environment

et al. 2013

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“…Based on the loss of detectable PrP Sc immunoreactivity after incubation at pH 11, it appears this effect may be largely pH-dependent. It is well established that prion infectivity is reduced under extremely basic conditions, such as exposure to NaOH (pH 12–14) [19-21]. While the pH generated by SPC-P is lower at 11, it appears to be a favorable characteristic of the compound with regard to PrP Sc protease sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Exposure of RML scrapie agent to a sodium percarbonate-based product and sodium dodecyl sulfate renders PrPScprotease sensitive but does not eliminate infectivity

et al. 2013

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BackgroundPrions, the causative agents of the transmissible spongiform encephalopathies, are notoriously difficult to inactivate. Current decontamination recommendations by the World Health Organization include prolonged exposure to 1 N sodium hydroxide or > 20,000 ppm sodium hypochlorite, or autoclaving. For decontamination of large stainless steel surfaces and equipment as in abattoirs, for example, these methods are harsh or unsuitable. The current study was designed to evaluate the effectiveness of a commercial product containing sodium percarbonate to inactivate prions. Samples of mouse brain infected with a mouse-adapted strain of the scrapie agent (RML) were exposed to a sodium percarbonate-based product (SPC-P). Treated samples were evaluated for abnormal prion protein (PrPSc)-immunoreactivity by western blot analysis, and residual infectivity by mouse bioassay.ResultsExposure to a 21% solution of SPC-P or a solution containing either 2.1% or 21% SPC-P in combination with sodium dodecyl sulfate (SDS) resulted in increased proteinase K sensitivity of PrPSc. Limited reductions in infectivity were observed depending on treatment condition. A marginal effect on infectivity was observed with SPC-P alone, but an approximate 2–3 log10 reduction was observed with the addition of SDS, though exposure to SDS alone resulted in an approximate 2 log10 reduction.ConclusionsThis study demonstrates that exposure of a mouse-adapted scrapie strain to SPC-P does not eliminate infectivity, but does render PrPSc protease sensitive.

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“…24 In recent studies, it could be proved that NaOH is effective to inactivate aberrantly folded cellular proteins (PrP sc , prions), causative agents for TSEs. [24][25][26] In consequence, PrP sc are eliminated and cross contaminations minimized. Furthermore, NaOH not only destroys PrP sc but also removes HIV, HAV, HBV and HCV, and DNA/RNA.…”

Section: Introductionmentioning

confidence: 97%